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A β-cell glycoprotein of Mr 40 000 is the major rat islet cell immunogen following xenogenic immunisation

Bækkeskov, S. and Lernmark, Å LU orcid (1984) In Diabetologia 27(1 Supplement). p.70-73
Abstract

An antiserum (R2) was raised in a rabbit against dispersed Sprague Dawley rat islet cells. The R2 antiserum contains islet cell surface antibodies, which mediate complement-dependent cytotoxicity against islet cells resulting in a block of glucose induced insulin release. Immunoprecipitation and gel electrophoretic analysis showed that R2 specifically recognizes an Mr 40 000 glycoprotein present in both rat islet and rat insulinoma cells. This glycoprotein is amphiphilic in character and probably represents a pancreatic β cell specific plasma membrane component. The results support previous observations in mouse β cells that a plasma membrane glycoprotein of Mr 40K constitutes a major islet cell immunogen.

Please use this url to cite or link to this publication:
author
and
publishing date
type
Contribution to journal
publication status
published
keywords
antiserum R2, Glycoprotein M 40 000, islet cell immunogen, rat, xenogenic immunisation
in
Diabetologia
volume
27
issue
1 Supplement
pages
4 pages
publisher
Springer
external identifiers
  • pmid:6383923
  • scopus:0021247311
ISSN
0012-186X
DOI
10.1007/BF00275650
language
English
LU publication?
no
id
2ea66a05-2b5b-4afc-8201-5e7ed3a6e574
date added to LUP
2019-09-16 12:42:48
date last changed
2024-03-13 08:18:58
@article{2ea66a05-2b5b-4afc-8201-5e7ed3a6e574,
  abstract     = {{<p>An antiserum (R2) was raised in a rabbit against dispersed Sprague Dawley rat islet cells. The R2 antiserum contains islet cell surface antibodies, which mediate complement-dependent cytotoxicity against islet cells resulting in a block of glucose induced insulin release. Immunoprecipitation and gel electrophoretic analysis showed that R2 specifically recognizes an M<sub>r</sub> 40 000 glycoprotein present in both rat islet and rat insulinoma cells. This glycoprotein is amphiphilic in character and probably represents a pancreatic β cell specific plasma membrane component. The results support previous observations in mouse β cells that a plasma membrane glycoprotein of M<sub>r</sub> 40K constitutes a major islet cell immunogen.</p>}},
  author       = {{Bækkeskov, S. and Lernmark, Å}},
  issn         = {{0012-186X}},
  keywords     = {{antiserum R2; Glycoprotein M 40 000; islet cell immunogen; rat; xenogenic immunisation}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{1 Supplement}},
  pages        = {{70--73}},
  publisher    = {{Springer}},
  series       = {{Diabetologia}},
  title        = {{A β-cell glycoprotein of M<sub>r</sub> 40 000 is the major rat islet cell immunogen following xenogenic immunisation}},
  url          = {{http://dx.doi.org/10.1007/BF00275650}},
  doi          = {{10.1007/BF00275650}},
  volume       = {{27}},
  year         = {{1984}},
}