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Stimulation of insulin release by thiols

Hellman, Bo ; Idahl, Lars Åke ; Lernmark, Åke LU ; Sehlin, Janove and Täljedal, Inge Bert (1975) In BBA - General Subjects 392(1). p.101-109
Abstract

The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to... (More)

The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mM D-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the β-cells.

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author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
in
BBA - General Subjects
volume
392
issue
1
pages
101 - 109
publisher
Elsevier
external identifiers
  • pmid:1092370
  • scopus:0016738060
ISSN
0304-4165
DOI
10.1016/0304-4165(75)90170-1
language
English
LU publication?
no
id
2f00b47c-5e25-4f89-b7e6-c75b94641fdc
date added to LUP
2019-09-18 12:14:25
date last changed
2020-09-27 07:18:31
@article{2f00b47c-5e25-4f89-b7e6-c75b94641fdc,
  abstract     = {<p>The effects of thiol compounds on insulin release were studied in microdissected pancreatic islets of non-inbred ob/ob mice. In control experiments the reactivity of thiols against 6,6′-dithiodinicotinic acid and the degradation of mouse insulin were measured. At a concentration of 0.1 mM, 1-thio-D-glucose or reduced glutathione potentiated the insulin-releasing action of 10 mM D-glucose without affecting glucose oxidation. When tested at a concentration equivalent to about 0.1 mM reactive thiol, dextran-linked L-cysteine also potentiated the glucose-induced insulin secretion. In microperifusion experiments the insulin-releasing action of 1-thio-D-glucose was found to exhibit a rapid onset followed by a decline of the secretory rate to values lower than those observed with 10 mM D-glucose alone. No thiol stimulated insulin release in the absence of glucose. It is suggested that thiol compounds stimulate insulin release by splitting membrane disulphides in the β-cells.</p>},
  author       = {Hellman, Bo and Idahl, Lars Åke and Lernmark, Åke and Sehlin, Janove and Täljedal, Inge Bert},
  issn         = {0304-4165},
  language     = {eng},
  month        = {05},
  number       = {1},
  pages        = {101--109},
  publisher    = {Elsevier},
  series       = {BBA - General Subjects},
  title        = {Stimulation of insulin release by thiols},
  url          = {http://dx.doi.org/10.1016/0304-4165(75)90170-1},
  doi          = {10.1016/0304-4165(75)90170-1},
  volume       = {392},
  year         = {1975},
}