Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus
(2023) In Methods in Molecular Biology 2674. p.261-282- Abstract
Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS,... (More)
Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS, including instructions for generating BMDMs and growing the bacterium. This protocol can easily be modified to other bacterial pathogens, or human macrophages.
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- author
- Valfridsson, Christine LU ; Westerlund, Elsa LU ; Hancz, Dóra LU and Persson, Jenny J. LU
- organization
- publishing date
- 2023
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Group A Streptococcus, Inflammasome, Murine bone marrow-derived macrophages
- host publication
- Methods in Molecular Biology
- series title
- Methods in Molecular Biology
- volume
- 2674
- pages
- 22 pages
- publisher
- Humana Press
- external identifiers
-
- pmid:37258974
- scopus:85160721274
- ISSN
- 1940-6029
- 1064-3745
- DOI
- 10.1007/978-1-0716-3243-7_18
- language
- English
- LU publication?
- yes
- id
- 2f37d44b-597a-4b5f-95ab-31d10ed57d4e
- date added to LUP
- 2023-08-31 15:34:39
- date last changed
- 2024-04-20 02:19:32
@inbook{2f37d44b-597a-4b5f-95ab-31d10ed57d4e, abstract = {{<p>Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS, including instructions for generating BMDMs and growing the bacterium. This protocol can easily be modified to other bacterial pathogens, or human macrophages.</p>}}, author = {{Valfridsson, Christine and Westerlund, Elsa and Hancz, Dóra and Persson, Jenny J.}}, booktitle = {{Methods in Molecular Biology}}, issn = {{1940-6029}}, keywords = {{Group A Streptococcus; Inflammasome; Murine bone marrow-derived macrophages}}, language = {{eng}}, pages = {{261--282}}, publisher = {{Humana Press}}, series = {{Methods in Molecular Biology}}, title = {{Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus}}, url = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_18}}, doi = {{10.1007/978-1-0716-3243-7_18}}, volume = {{2674}}, year = {{2023}}, }