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Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus

Valfridsson, Christine LU ; Westerlund, Elsa LU ; Hancz, Dóra LU and Persson, Jenny J. LU (2023) In Methods in Molecular Biology 2674. p.261-282
Abstract

Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS,... (More)

Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS, including instructions for generating BMDMs and growing the bacterium. This protocol can easily be modified to other bacterial pathogens, or human macrophages.

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Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Group A Streptococcus, Inflammasome, Murine bone marrow-derived macrophages
host publication
Methods in Molecular Biology
series title
Methods in Molecular Biology
volume
2674
pages
22 pages
publisher
Humana Press
external identifiers
  • pmid:37258974
  • scopus:85160721274
ISSN
1940-6029
1064-3745
DOI
10.1007/978-1-0716-3243-7_18
language
English
LU publication?
yes
id
2f37d44b-597a-4b5f-95ab-31d10ed57d4e
date added to LUP
2023-08-31 15:34:39
date last changed
2024-04-20 02:19:32
@inbook{2f37d44b-597a-4b5f-95ab-31d10ed57d4e,
  abstract     = {{<p>Inflammasomes are large multiprotein complexes that assemble mainly in innate immune cells after detection of microbial or sterile insults. Activation of inflammasomes is a key proinflammatory event during infection, and many pathogens have evolved specific evasion mechanisms to evade or inhibit inflammasome activation. One such pathogen is the common bacterium group A Streptococcus (GAS), which causes a wide range of diseases of varying severity. GAS secretes a multitude of virulence factors whereof the pore-forming protein streptolysin O (SLO) is the main inflammasome activation determinant. Here we provide a protocol for reliable evaluation of inflammasome activation in murine bone marrow-derived macrophages (BMDM) infected with GAS, including instructions for generating BMDMs and growing the bacterium. This protocol can easily be modified to other bacterial pathogens, or human macrophages.</p>}},
  author       = {{Valfridsson, Christine and Westerlund, Elsa and Hancz, Dóra and Persson, Jenny J.}},
  booktitle    = {{Methods in Molecular Biology}},
  issn         = {{1940-6029}},
  keywords     = {{Group A Streptococcus; Inflammasome; Murine bone marrow-derived macrophages}},
  language     = {{eng}},
  pages        = {{261--282}},
  publisher    = {{Humana Press}},
  series       = {{Methods in Molecular Biology}},
  title        = {{Detection of Inflammasome Activation in Murine Bone Marrow-Derived Macrophages Infected with Group A Streptococcus}},
  url          = {{http://dx.doi.org/10.1007/978-1-0716-3243-7_18}},
  doi          = {{10.1007/978-1-0716-3243-7_18}},
  volume       = {{2674}},
  year         = {{2023}},
}