Advanced

Continuous single sensillum recording as a detection method for moth pheromone components in the effluent of a gas chromatograph

Van der Pers, Jan N.C. and Löfstedt, Christer LU (1983) In Physiological Entomology 8(2). p.203-211
Abstract

ABSTRACT. Single sensillum recordings were made from male antennal pheromone receptors of Agrotis segetum (Schiff.) (Noctuidae) and Adoxophyes orana (F.v.R.) (Tortricidae). A gas chromatograph (GC) was used to prepare and to deliver the odour stimuli. Samples of female abdominal gland extracts were injected into the column of the GC and the responses of the receptor cells to the effluent were recorded continuously. The receptor cells responded with an increase of their action potential frequency during elution of the major pheromone components. The pheromone receptors of Agrotis showed a much higher sensitivity than those of Adoxophyes. In the extract of female glands from Agrotis, compounds were detected which have not been identified... (More)

ABSTRACT. Single sensillum recordings were made from male antennal pheromone receptors of Agrotis segetum (Schiff.) (Noctuidae) and Adoxophyes orana (F.v.R.) (Tortricidae). A gas chromatograph (GC) was used to prepare and to deliver the odour stimuli. Samples of female abdominal gland extracts were injected into the column of the GC and the responses of the receptor cells to the effluent were recorded continuously. The receptor cells responded with an increase of their action potential frequency during elution of the major pheromone components. The pheromone receptors of Agrotis showed a much higher sensitivity than those of Adoxophyes. In the extract of female glands from Agrotis, compounds were detected which have not been identified in previous studies of the sex pheromone of this species. It is suggested that the combination of GC techniques with direct single sensillum recording may serve as a valuable supplement to electro‐antennographic techniques.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adoxophyes orana, Agrotis segetum, antennae, gas chromatography, Lepidoptera, pheromone detection, single‐sensillum recording
in
Physiological Entomology
volume
8
issue
2
pages
203 - 211
publisher
Wiley-Blackwell
external identifiers
  • scopus:84981580053
ISSN
0307-6962
DOI
10.1111/j.1365-3032.1983.tb00350.x
project
Evolutionary mechanisms of pheromone divergence in Lepidoptera
language
English
LU publication?
yes
id
2f560f88-6801-4a01-b9ee-a1835cd4844f
date added to LUP
2020-07-23 15:57:19
date last changed
2020-09-23 15:07:21
@article{2f560f88-6801-4a01-b9ee-a1835cd4844f,
  abstract     = {<p>ABSTRACT.  Single sensillum recordings were made from male antennal pheromone receptors of Agrotis segetum (Schiff.) (Noctuidae) and Adoxophyes orana (F.v.R.) (Tortricidae). A gas chromatograph (GC) was used to prepare and to deliver the odour stimuli. Samples of female abdominal gland extracts were injected into the column of the GC and the responses of the receptor cells to the effluent were recorded continuously. The receptor cells responded with an increase of their action potential frequency during elution of the major pheromone components. The pheromone receptors of Agrotis showed a much higher sensitivity than those of Adoxophyes. In the extract of female glands from Agrotis, compounds were detected which have not been identified in previous studies of the sex pheromone of this species. It is suggested that the combination of GC techniques with direct single sensillum recording may serve as a valuable supplement to electro‐antennographic techniques.</p>},
  author       = {Van der Pers, Jan N.C. and Löfstedt, Christer},
  issn         = {0307-6962},
  language     = {eng},
  month        = {01},
  number       = {2},
  pages        = {203--211},
  publisher    = {Wiley-Blackwell},
  series       = {Physiological Entomology},
  title        = {Continuous single sensillum recording as a detection method for moth pheromone components in the effluent of a gas chromatograph},
  url          = {http://dx.doi.org/10.1111/j.1365-3032.1983.tb00350.x},
  doi          = {10.1111/j.1365-3032.1983.tb00350.x},
  volume       = {8},
  year         = {1983},
}