The effect of leucyl-leucine methyl ester on proliferation and Ig secretion of EBV-transformed B lymphocytes.
(1989) In Immunology 66(4). p.485-490- Abstract
- The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin... (More)
- The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin (Ig) production (three to 11 times) compared to non-treated but similarly infected lymphocytes. Since the effect of leucyl-leucine methyl ester treatment was also detectable in low-density (100 B cells/well) cultures, the suppression was unlikely to be exerted by EBV-specific T-cell clones, but pointed rather to the natural killer (NK) cells as effectors. (Less)
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- author
- Ohlin, Mats LU ; Danielsson, Lena LU ; Carlsson, Roland LU and Borrebaeck, Carl LU
- organization
- publishing date
- 1989
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Immunology
- volume
- 66
- issue
- 4
- pages
- 485 - 490
- publisher
- Wiley-Blackwell
- ISSN
- 0019-2805
- language
- English
- LU publication?
- yes
- id
- 2f9064e0-024b-49dc-9bec-e566a8b2f2e4
- alternative location
- https://ludwig.lub.lu.se/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,uid&db=a9h&AN=16019690&site=eds-live&scope=site
- date added to LUP
- 2022-11-30 08:48:30
- date last changed
- 2022-11-30 15:36:57
@article{2f9064e0-024b-49dc-9bec-e566a8b2f2e4, abstract = {{The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin (Ig) production (three to 11 times) compared to non-treated but similarly infected lymphocytes. Since the effect of leucyl-leucine methyl ester treatment was also detectable in low-density (100 B cells/well) cultures, the suppression was unlikely to be exerted by EBV-specific T-cell clones, but pointed rather to the natural killer (NK) cells as effectors.}}, author = {{Ohlin, Mats and Danielsson, Lena and Carlsson, Roland and Borrebaeck, Carl}}, issn = {{0019-2805}}, language = {{eng}}, number = {{4}}, pages = {{485--490}}, publisher = {{Wiley-Blackwell}}, series = {{Immunology}}, title = {{The effect of leucyl-leucine methyl ester on proliferation and Ig secretion of EBV-transformed B lymphocytes.}}, url = {{https://ludwig.lub.lu.se/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,uid&db=a9h&AN=16019690&site=eds-live&scope=site}}, volume = {{66}}, year = {{1989}}, }