Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

The effect of leucyl-leucine methyl ester on proliferation and Ig secretion of EBV-transformed B lymphocytes.

Ohlin, Mats LU orcid ; Danielsson, Lena LU ; Carlsson, Roland LU and Borrebaeck, Carl LU (1989) In Immunology 66(4). p.485-490
Abstract
The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin... (More)
The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin (Ig) production (three to 11 times) compared to non-treated but similarly infected lymphocytes. Since the effect of leucyl-leucine methyl ester treatment was also detectable in low-density (100 B cells/well) cultures, the suppression was unlikely to be exerted by EBV-specific T-cell clones, but pointed rather to the natural killer (NK) cells as effectors. (Less)
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Immunology
volume
66
issue
4
pages
485 - 490
publisher
Wiley-Blackwell
ISSN
0019-2805
language
English
LU publication?
yes
id
2f9064e0-024b-49dc-9bec-e566a8b2f2e4
alternative location
https://ludwig.lub.lu.se/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,uid&db=a9h&AN=16019690&site=eds-live&scope=site
date added to LUP
2022-11-30 08:48:30
date last changed
2022-11-30 15:36:57
@article{2f9064e0-024b-49dc-9bec-e566a8b2f2e4,
  abstract     = {{The selective cytotoxicity of the lysosomotropic methyl esters of leucine or its lysosomal condensation product leucyl-leucine has been used to investigate the effect of cytolytic cells on the clonal outgrowth, cellular proliferation and antibody secretion of Epstein-Barr virus (EBV)-transformed human B cells. Large granular lymphocytes (LGL), monocytes, and a subset of T cells (CD8/CD11+) were permanently eliminated by the ester treatment. These lysosome-rich cells severely inhibit the clonal outgrowth of EBV-infected B cells, as determined by Poisson distribution calculations. Furthermore, leucyl-leucine methyl ester-treated and EBV-infected lymphocytes showed a significant increase in proliferative capability as well as immunoglobulin (Ig) production (three to 11 times) compared to non-treated but similarly infected lymphocytes. Since the effect of leucyl-leucine methyl ester treatment was also detectable in low-density (100 B cells/well) cultures, the suppression was unlikely to be exerted by EBV-specific T-cell clones, but pointed rather to the natural killer (NK) cells as effectors.}},
  author       = {{Ohlin, Mats and Danielsson, Lena and Carlsson, Roland and Borrebaeck, Carl}},
  issn         = {{0019-2805}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{485--490}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Immunology}},
  title        = {{The effect of leucyl-leucine methyl ester on proliferation and Ig secretion of EBV-transformed B lymphocytes.}},
  url          = {{https://ludwig.lub.lu.se/login?url=https://search.ebscohost.com/login.aspx?direct=true&AuthType=ip,uid&db=a9h&AN=16019690&site=eds-live&scope=site}},
  volume       = {{66}},
  year         = {{1989}},
}