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Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing

Drissen, Roy ; Buza-Vidas, Natalija LU ; Woll, Petter ; Thongjuea, Supat ; Gambardella, Adriana ; Giustacchini, Alice ; Mancini, Elena ; Zriwil, Alya LU ; Lutteropp, Michael and Grover, Amit , et al. (2016) In Nature Immunology 17(6). p.666-676
Abstract

According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcγRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor... (More)

According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcγRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor GATA-1 generated mast cells, eosinophils, megakaryocytes and erythroid cells, and a pathway lacking expression of that gene generated monocytes, neutrophils and lymphocytes. These results identify an early hematopoietic-lineage bifurcation that separates the myeloid lineages before their segregation from other hematopoietic-lineage potential.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Nature Immunology
volume
17
issue
6
pages
666 - 676
publisher
Nature Publishing Group
external identifiers
  • scopus:84962050673
  • wos:000376162200011
  • pmid:27043410
ISSN
1529-2908
DOI
10.1038/ni.3412
language
English
LU publication?
yes
id
2f9395d9-f6c6-4e2f-9471-fbc29faba019
date added to LUP
2016-04-27 13:37:21
date last changed
2025-03-23 11:13:35
@article{2f9395d9-f6c6-4e2f-9471-fbc29faba019,
  abstract     = {{<p>According to current models of hematopoiesis, lymphoid-primed multi-potent progenitors (LMPPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)Flt3(hi)) and common myeloid progenitors (CMPs) (Lin(-)Sca-1(+)c-Kit(+)CD34(+)CD41(hi)) establish an early branch point for separate lineage-commitment pathways from hematopoietic stem cells, with the notable exception that both pathways are proposed to generate all myeloid innate immune cell types through the same myeloid-restricted pre-granulocyte-macrophage progenitor (pre-GM) (Lin(-)Sca-1(-)c-Kit(+)CD41(-)FcγRII/III(-)CD150(-)CD105(-)). By single-cell transcriptome profiling of pre-GMs, we identified distinct myeloid differentiation pathways: a pathway expressing the gene encoding the transcription factor GATA-1 generated mast cells, eosinophils, megakaryocytes and erythroid cells, and a pathway lacking expression of that gene generated monocytes, neutrophils and lymphocytes. These results identify an early hematopoietic-lineage bifurcation that separates the myeloid lineages before their segregation from other hematopoietic-lineage potential.</p>}},
  author       = {{Drissen, Roy and Buza-Vidas, Natalija and Woll, Petter and Thongjuea, Supat and Gambardella, Adriana and Giustacchini, Alice and Mancini, Elena and Zriwil, Alya and Lutteropp, Michael and Grover, Amit and Mead, Adam and Sitnicka, Ewa and Jacobsen, Sten Eirik W and Nerlov, Claus}},
  issn         = {{1529-2908}},
  language     = {{eng}},
  number       = {{6}},
  pages        = {{666--676}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Immunology}},
  title        = {{Distinct myeloid progenitor-differentiation pathways identified through single-cell RNA sequencing}},
  url          = {{http://dx.doi.org/10.1038/ni.3412}},
  doi          = {{10.1038/ni.3412}},
  volume       = {{17}},
  year         = {{2016}},
}