Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

In vivo mallard experiments indicate that zanamivir has less potential for environmental influenza a virus resistance development than oseltamivir

Nykvist, Marie ; Gillman, Anna ; Lindström, Hanna Söderström ; Tang, Chaojun ; Fedorova, Ganna ; Lundkvist, Åke ; Latorre-Margalef, Neus LU ; Wille, Michelle and Järhult, Josef D. (2017) In Journal of General Virology 98(12). p.2937-2949
Abstract

Neuraminidase inhibitors are a cornerstone of influenza pandemic preparedness before vaccines can be mass-produced and thus a neuraminidase inhibitor-resistant pandemic is a serious threat to public health. Earlier work has demonstrated the potential for development and persistence of oseltamivir resistance in influenza A viruses exposed to environmentally relevant water concentrations of the drug when infecting mallards, the natural influenza reservoir that serves as the genetic base for human pandemics. As zanamivir is the major second-line neuraminidase inhibitor treatment, this study aimed to assess the potential for development and persistence of zanamivir resistance in an in vivo mallard model; especially important as zanamivir... (More)

Neuraminidase inhibitors are a cornerstone of influenza pandemic preparedness before vaccines can be mass-produced and thus a neuraminidase inhibitor-resistant pandemic is a serious threat to public health. Earlier work has demonstrated the potential for development and persistence of oseltamivir resistance in influenza A viruses exposed to environmentally relevant water concentrations of the drug when infecting mallards, the natural influenza reservoir that serves as the genetic base for human pandemics. As zanamivir is the major second-line neuraminidase inhibitor treatment, this study aimed to assess the potential for development and persistence of zanamivir resistance in an in vivo mallard model; especially important as zanamivir will probably be increasingly used. Our results indicate less potential for development and persistence of resistance due to zanamivir than oseltamivir in an environmental setting. This conclusion is based on: (1) the lower increase in zanamivir IC50 conferred by the mutations caused by zanamivir exposure (2–17-fold); (2) the higher zanamivir water concentration needed to induce resistance (at least 10 μg l-1); (3) the lack of zanamivir resistance persistence without drug pressure; and (4) the multiple resistance-related substitutions seen during zanamivir exposure (V116A, A138V, R152K, T157I and D199G) suggesting lack of one straight-forward evolutionary path to resistance. Our study also adds further evidence regarding the stability of the oseltamivir-induced substitution H275Y without drug pressure, and demonstrates the ability of a H275Y-carrying virus to acquire secondary mutations, further boosting oseltamivir resistance when exposed to zanamivir. Similar studies using influenza A viruses of the N2-phylogenetic group of neuraminidases are recommended.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antiviral resistance, Avian influenza, Drug residues, Neuraminidase inhibitor, Pandemic preparedness, Relenza
in
Journal of General Virology
volume
98
issue
12
pages
13 pages
publisher
Microbiology Society
external identifiers
  • scopus:85037721670
  • pmid:29139346
ISSN
0022-1317
DOI
10.1099/jgv.0.000977
language
English
LU publication?
no
id
2fa81e0d-1447-45ba-8986-28b928b6ee91
date added to LUP
2018-03-06 16:29:56
date last changed
2024-01-14 16:21:58
@article{2fa81e0d-1447-45ba-8986-28b928b6ee91,
  abstract     = {{<p>Neuraminidase inhibitors are a cornerstone of influenza pandemic preparedness before vaccines can be mass-produced and thus a neuraminidase inhibitor-resistant pandemic is a serious threat to public health. Earlier work has demonstrated the potential for development and persistence of oseltamivir resistance in influenza A viruses exposed to environmentally relevant water concentrations of the drug when infecting mallards, the natural influenza reservoir that serves as the genetic base for human pandemics. As zanamivir is the major second-line neuraminidase inhibitor treatment, this study aimed to assess the potential for development and persistence of zanamivir resistance in an in vivo mallard model; especially important as zanamivir will probably be increasingly used. Our results indicate less potential for development and persistence of resistance due to zanamivir than oseltamivir in an environmental setting. This conclusion is based on: (1) the lower increase in zanamivir IC50 conferred by the mutations caused by zanamivir exposure (2–17-fold); (2) the higher zanamivir water concentration needed to induce resistance (at least 10 μg l<sup>-1</sup>); (3) the lack of zanamivir resistance persistence without drug pressure; and (4) the multiple resistance-related substitutions seen during zanamivir exposure (V116A, A138V, R152K, T157I and D199G) suggesting lack of one straight-forward evolutionary path to resistance. Our study also adds further evidence regarding the stability of the oseltamivir-induced substitution H275Y without drug pressure, and demonstrates the ability of a H275Y-carrying virus to acquire secondary mutations, further boosting oseltamivir resistance when exposed to zanamivir. Similar studies using influenza A viruses of the N2-phylogenetic group of neuraminidases are recommended.</p>}},
  author       = {{Nykvist, Marie and Gillman, Anna and Lindström, Hanna Söderström and Tang, Chaojun and Fedorova, Ganna and Lundkvist, Åke and Latorre-Margalef, Neus and Wille, Michelle and Järhult, Josef D.}},
  issn         = {{0022-1317}},
  keywords     = {{Antiviral resistance; Avian influenza; Drug residues; Neuraminidase inhibitor; Pandemic preparedness; Relenza}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  pages        = {{2937--2949}},
  publisher    = {{Microbiology Society}},
  series       = {{Journal of General Virology}},
  title        = {{In vivo mallard experiments indicate that zanamivir has less potential for environmental influenza a virus resistance development than oseltamivir}},
  url          = {{http://dx.doi.org/10.1099/jgv.0.000977}},
  doi          = {{10.1099/jgv.0.000977}},
  volume       = {{98}},
  year         = {{2017}},
}