Advanced

Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops

Pucetaite, Milda LU ; Velicka, Martynas; Pilipavicius, Jurgis; Beganskiene, Aldona; Ceponkus, Justinas and Sablinskas, Valdas (2016) In Journal of Raman Spectroscopy 47(6). p.681-686
Abstract
Uric acid concentration in human bodily fluids is an important marker for disorders such as gout, pre‐eclamsia or cardiovascular disease. However, currently used methods for its detection either lack sensitivity or require sophisticated, bulky and expensive equipment. In this work, we show that by using surface‐enhanced Raman scattering spectroscopy (SERS) on dried Ag colloidal drops and with 1064 nm excitation, concentrations of uric acid in aqueous solutions down to 10−6 M can be detected. Such sensitivity is sufficient for medical applications as concentration of uric acid in various bodily fluids are in the range of 10−3–10−4 M. Drying of the colloidal drops is known to result in the formation of ‘coffee‐ring’ structures that allow... (More)
Uric acid concentration in human bodily fluids is an important marker for disorders such as gout, pre‐eclamsia or cardiovascular disease. However, currently used methods for its detection either lack sensitivity or require sophisticated, bulky and expensive equipment. In this work, we show that by using surface‐enhanced Raman scattering spectroscopy (SERS) on dried Ag colloidal drops and with 1064 nm excitation, concentrations of uric acid in aqueous solutions down to 10−6 M can be detected. Such sensitivity is sufficient for medical applications as concentration of uric acid in various bodily fluids are in the range of 10−3–10−4 M. Drying of the colloidal drops is known to result in the formation of ‘coffee‐ring’ structures that allow obtaining high enhancements but poor reproducibility. Here, the formation of the structures was avoided by choosing aluminum oxide as a base substrate and by controlling environment conditions. Despite the fact that variations of signal enhancement from sample to sample prevent quantitative analysis from being performed, the results of this work imply that strict control of sample preparation conditions could lead to obtaining reproducible SERS enhancements. Results of density functional theory calculations of uric acid tautomer – five‐atom silver cluster complexes performed for the first time show that the differences between Raman and SERS spectra of uric acid can be mainly explained by tautomerization of the molecule and its bonding to the silver surface. Assignment of spectral bands is important for correct SERS signal interpretation and detection of uric acid in biological fluids in the future studies. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
in
Journal of Raman Spectroscopy
volume
47
issue
6
pages
681 - 686
publisher
John Wiley & Sons
external identifiers
  • scopus:84954271361
ISSN
1097-4555
DOI
10.1002/jrs.4875|
language
English
LU publication?
no
id
2fb9434d-a58a-448b-a5d1-6c4b7631d908
date added to LUP
2019-03-25 16:50:48
date last changed
2019-08-14 04:34:15
@article{2fb9434d-a58a-448b-a5d1-6c4b7631d908,
  abstract     = {Uric acid concentration in human bodily fluids is an important marker for disorders such as gout, pre‐eclamsia or cardiovascular disease. However, currently used methods for its detection either lack sensitivity or require sophisticated, bulky and expensive equipment. In this work, we show that by using surface‐enhanced Raman scattering spectroscopy (SERS) on dried Ag colloidal drops and with 1064 nm excitation, concentrations of uric acid in aqueous solutions down to 10−6 M can be detected. Such sensitivity is sufficient for medical applications as concentration of uric acid in various bodily fluids are in the range of 10−3–10−4 M. Drying of the colloidal drops is known to result in the formation of ‘coffee‐ring’ structures that allow obtaining high enhancements but poor reproducibility. Here, the formation of the structures was avoided by choosing aluminum oxide as a base substrate and by controlling environment conditions. Despite the fact that variations of signal enhancement from sample to sample prevent quantitative analysis from being performed, the results of this work imply that strict control of sample preparation conditions could lead to obtaining reproducible SERS enhancements. Results of density functional theory calculations of uric acid tautomer – five‐atom silver cluster complexes performed for the first time show that the differences between Raman and SERS spectra of uric acid can be mainly explained by tautomerization of the molecule and its bonding to the silver surface. Assignment of spectral bands is important for correct SERS signal interpretation and detection of uric acid in biological fluids in the future studies. },
  author       = {Pucetaite, Milda and Velicka, Martynas and Pilipavicius, Jurgis and Beganskiene, Aldona and Ceponkus, Justinas and Sablinskas, Valdas},
  issn         = {1097-4555},
  language     = {eng},
  number       = {6},
  pages        = {681--686},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Raman Spectroscopy},
  title        = {Uric acid detection by means of SERS spectroscopy on dried Ag colloidal drops},
  url          = {http://dx.doi.org/10.1002/jrs.4875|},
  volume       = {47},
  year         = {2016},
}