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Characterization of calcifications in posterior horn of human meniscus using micro-computed tomography

Karjalainen, Ville Pauli ; Hellberg, Iida ; Turkiewicz, Aleksandra LU ; Shakya, Bijay ; Khoshimova, Nodira ; Nevanranta, Eeva ; Gupta, Shuvashis Das LU orcid ; Elkhouly, Khaled ; Sjögren, Amanda LU orcid and Finnilä, Mikko A.J. , et al. (2026) In Osteoarthritis and Cartilage Open 8(3).
Abstract

Objective: Meniscal calcifications are associated with meniscal degeneration and osteoarthritis (OA). We investigated micro-computed tomography (μCT) imaging for identification of calcification patterns caused by basic calcium phosphate (BCP) and calcium pyrophosphate (CPP). Design: Posterior horns of human medial and lateral menisci from 19 individuals with medial compartment knee OA and 21 deceased donors were imaged with high-resolution μCT. Raman spectroscopy characterized the calcification types from histological sections, adjacent to μCT piece. Qualitative and quantitative analysis, visualization, and grading were performed using 3D μCT images of meniscal calcifications. Results: Different calcification patterns were observed with... (More)

Objective: Meniscal calcifications are associated with meniscal degeneration and osteoarthritis (OA). We investigated micro-computed tomography (μCT) imaging for identification of calcification patterns caused by basic calcium phosphate (BCP) and calcium pyrophosphate (CPP). Design: Posterior horns of human medial and lateral menisci from 19 individuals with medial compartment knee OA and 21 deceased donors were imaged with high-resolution μCT. Raman spectroscopy characterized the calcification types from histological sections, adjacent to μCT piece. Qualitative and quantitative analysis, visualization, and grading were performed using 3D μCT images of meniscal calcifications. Results: Different calcification patterns were observed with BCP and CPP. BCP was found at the borders of meniscal tissue and inside complex tears or fibrillation. In contrast, CPP accumulated as circumferential rod-like structures between collagen bundles, mainly inside the meniscal tissue, and lacked the porous 3D structure observed in BCP calcifications. Quantitatively, CPP samples had a higher calcification volume compared to BCP with a geometric mean ratio of 14 (95%CI; 3,73), and larger particle sizes with a ratio of 7 (95%CI; 2,8). Additionally, BCP calcifications had an organized porous structure with a closed porosity range of 6–19%, while a similar structure was not seen in CPPs (range 1–4.5%). Conclusions: We qualitatively and quantitatively identified volumetric and morphological differences in the calcification deposition patterns between BCP and CPP calcifications in human meniscus. Ultimately, this μCT imaging method supports more detailed characterization of calcifications in OA and helps to pave the way for identifying potential biomarkers and understanding the mechanistic models of disease progression related to pathological calcifications better.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Basic calcium phosphate, Calcification, Calcium pyrophosphate, Meniscus, Micro-computed tomography, Osteoarthritis
in
Osteoarthritis and Cartilage Open
volume
8
issue
3
article number
100820
publisher
Elsevier
external identifiers
  • scopus:105039963207
  • pmid:42254336
ISSN
2665-9131
DOI
10.1016/j.ocarto.2026.100820
language
English
LU publication?
yes
additional info
Publisher Copyright: © 2026 The Authors
id
2fe1f93c-340a-45cf-9feb-8b9103ee9f36
date added to LUP
2026-06-12 10:54:44
date last changed
2026-08-08 20:40:12
@article{2fe1f93c-340a-45cf-9feb-8b9103ee9f36,
  abstract     = {{<p>Objective: Meniscal calcifications are associated with meniscal degeneration and osteoarthritis (OA). We investigated micro-computed tomography (μCT) imaging for identification of calcification patterns caused by basic calcium phosphate (BCP) and calcium pyrophosphate (CPP). Design: Posterior horns of human medial and lateral menisci from 19 individuals with medial compartment knee OA and 21 deceased donors were imaged with high-resolution μCT. Raman spectroscopy characterized the calcification types from histological sections, adjacent to μCT piece. Qualitative and quantitative analysis, visualization, and grading were performed using 3D μCT images of meniscal calcifications. Results: Different calcification patterns were observed with BCP and CPP. BCP was found at the borders of meniscal tissue and inside complex tears or fibrillation. In contrast, CPP accumulated as circumferential rod-like structures between collagen bundles, mainly inside the meniscal tissue, and lacked the porous 3D structure observed in BCP calcifications. Quantitatively, CPP samples had a higher calcification volume compared to BCP with a geometric mean ratio of 14 (95%CI; 3,73), and larger particle sizes with a ratio of 7 (95%CI; 2,8). Additionally, BCP calcifications had an organized porous structure with a closed porosity range of 6–19%, while a similar structure was not seen in CPPs (range 1–4.5%). Conclusions: We qualitatively and quantitatively identified volumetric and morphological differences in the calcification deposition patterns between BCP and CPP calcifications in human meniscus. Ultimately, this μCT imaging method supports more detailed characterization of calcifications in OA and helps to pave the way for identifying potential biomarkers and understanding the mechanistic models of disease progression related to pathological calcifications better.</p>}},
  author       = {{Karjalainen, Ville Pauli and Hellberg, Iida and Turkiewicz, Aleksandra and Shakya, Bijay and Khoshimova, Nodira and Nevanranta, Eeva and Gupta, Shuvashis Das and Elkhouly, Khaled and Sjögren, Amanda and Finnilä, Mikko A.J. and Önnerfjord, Patrik and Hughes, Velocity and Tjörnstrand, Jon and Englund, Martin and Saarakkala, Simo}},
  issn         = {{2665-9131}},
  keywords     = {{Basic calcium phosphate; Calcification; Calcium pyrophosphate; Meniscus; Micro-computed tomography; Osteoarthritis}},
  language     = {{eng}},
  number       = {{3}},
  publisher    = {{Elsevier}},
  series       = {{Osteoarthritis and Cartilage Open}},
  title        = {{Characterization of calcifications in posterior horn of human meniscus using micro-computed tomography}},
  url          = {{http://dx.doi.org/10.1016/j.ocarto.2026.100820}},
  doi          = {{10.1016/j.ocarto.2026.100820}},
  volume       = {{8}},
  year         = {{2026}},
}