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Physiological requirements for growth and competitiveness of Dekkera bruxellensis under oxygen-limited or anaerobic conditions

Blomqvist, Johanna; Sanchez Nogue, Violeta LU ; Gorwa-Grauslund, Marie-Francoise LU and Passoth, Volkmar (2012) In Yeast 29(7). p.265-274
Abstract
The effect of glucose and oxygen limitation on the growth and fermentation performances of Dekkera bruxellensis was investigated in order to understand which factors favour its propagation in ethanol or wine plants. Although D. bruxellensis has been described as a facultative anaerobe, no growth was observed in mineral medium under complete anaerobiosis while growth was retarded under severe oxygen limitation. In a continuous culture with no gas inflow, glucose was not completely consumed, most probably due to oxygen limitation. When an air/nitrogen mixture (O-2-content ca. 5%) was sparged to the culture, growth became glucose-limited. In co-cultivations with Saccharomyces cerevisiae, ethanol yields/g consumed sugar were not affected by... (More)
The effect of glucose and oxygen limitation on the growth and fermentation performances of Dekkera bruxellensis was investigated in order to understand which factors favour its propagation in ethanol or wine plants. Although D. bruxellensis has been described as a facultative anaerobe, no growth was observed in mineral medium under complete anaerobiosis while growth was retarded under severe oxygen limitation. In a continuous culture with no gas inflow, glucose was not completely consumed, most probably due to oxygen limitation. When an air/nitrogen mixture (O-2-content ca. 5%) was sparged to the culture, growth became glucose-limited. In co-cultivations with Saccharomyces cerevisiae, ethanol yields/g consumed sugar were not affected by the co-cultures as compared to the pure cultures. However, different population responses were observed in both systems. In oxygen-limited cultivation, glucose was depleted within 24 h after challenging with S. cerevisiae and both yeast populations were maintained at a stable level. In contrast, the S. cerevisiae population constantly decreased to about 1% of its initial cell number in the sparged glucose-limited fermentation, whereas the D. bruxellensis population remained constant. To identify the requirements of D. bruxellensis for anaerobic growth, the yeast was cultivated in several nitrogen sources and with the addition of amino acids. Yeast extract and most of the supplied amino acids supported anaerobic growth, which points towards a higher nutrient demand for D. bruxellensis compared to S. cerevisiae in anaerobic conditions. Copyright (c) 2012 John Wiley & Sons, Ltd. (Less)
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author
organization
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Contribution to journal
publication status
published
subject
keywords
Dekkera bruxellensis, Saccharomyces cerevisiae, anaerobic growth, ethanol production, competition, continuous cultivation
in
Yeast
volume
29
issue
7
pages
265 - 274
publisher
John Wiley & Sons
external identifiers
  • wos:000306014900002
  • scopus:84863554583
ISSN
1097-0061
DOI
10.1002/yea.2904
language
English
LU publication?
yes
id
8ee1bb3a-5f69-4256-81d5-0ed513bdd42c (old id 3001533)
date added to LUP
2012-08-21 11:25:47
date last changed
2017-08-27 04:32:01
@article{8ee1bb3a-5f69-4256-81d5-0ed513bdd42c,
  abstract     = {The effect of glucose and oxygen limitation on the growth and fermentation performances of Dekkera bruxellensis was investigated in order to understand which factors favour its propagation in ethanol or wine plants. Although D. bruxellensis has been described as a facultative anaerobe, no growth was observed in mineral medium under complete anaerobiosis while growth was retarded under severe oxygen limitation. In a continuous culture with no gas inflow, glucose was not completely consumed, most probably due to oxygen limitation. When an air/nitrogen mixture (O-2-content ca. 5%) was sparged to the culture, growth became glucose-limited. In co-cultivations with Saccharomyces cerevisiae, ethanol yields/g consumed sugar were not affected by the co-cultures as compared to the pure cultures. However, different population responses were observed in both systems. In oxygen-limited cultivation, glucose was depleted within 24 h after challenging with S. cerevisiae and both yeast populations were maintained at a stable level. In contrast, the S. cerevisiae population constantly decreased to about 1% of its initial cell number in the sparged glucose-limited fermentation, whereas the D. bruxellensis population remained constant. To identify the requirements of D. bruxellensis for anaerobic growth, the yeast was cultivated in several nitrogen sources and with the addition of amino acids. Yeast extract and most of the supplied amino acids supported anaerobic growth, which points towards a higher nutrient demand for D. bruxellensis compared to S. cerevisiae in anaerobic conditions. Copyright (c) 2012 John Wiley & Sons, Ltd.},
  author       = {Blomqvist, Johanna and Sanchez Nogue, Violeta and Gorwa-Grauslund, Marie-Francoise and Passoth, Volkmar},
  issn         = {1097-0061},
  keyword      = {Dekkera bruxellensis,Saccharomyces cerevisiae,anaerobic growth,ethanol production,competition,continuous cultivation},
  language     = {eng},
  number       = {7},
  pages        = {265--274},
  publisher    = {John Wiley & Sons},
  series       = {Yeast},
  title        = {Physiological requirements for growth and competitiveness of Dekkera bruxellensis under oxygen-limited or anaerobic conditions},
  url          = {http://dx.doi.org/10.1002/yea.2904},
  volume       = {29},
  year         = {2012},
}