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Lymph node tissue kallikrein-related peptidase 6 mRNA: a progression marker for colorectal cancer

Ohlsson, L.; Lindmark, Gudrun LU ; Israelsson, A.; Palmqvist, R.; Oberg, A.; Hammarstrom, M-L and Hammarstrom, S. (2012) In British Journal of Cancer 107(1). p.150-157
Abstract
BACKGROUND: A most important characteristic feature for poor prognosis in colorectal cancer (CRC) is the presence of lymph node metastasis. Determination of carcinoembryonic antigen (CEA) mRNA levels in lymph nodes has proven powerful for quantification of disseminated tumour cells. Here, we investigate the utility of human tissue kallikrein-related peptidase 6 (KLK6) mRNA as a progression biomarker to complement CEA mRNA, for improved selection of patients in need of adjuvant therapy and intensified follow-up after surgery. METHODS: Lymph nodes of pTNM stage I-IV CRC-(166 patients/503 lymph nodes) and control (23/108) patients were collected at surgery and analysed by quantitative RT-PCR. RESULTS: Lymph node KLK6 positivity was an... (More)
BACKGROUND: A most important characteristic feature for poor prognosis in colorectal cancer (CRC) is the presence of lymph node metastasis. Determination of carcinoembryonic antigen (CEA) mRNA levels in lymph nodes has proven powerful for quantification of disseminated tumour cells. Here, we investigate the utility of human tissue kallikrein-related peptidase 6 (KLK6) mRNA as a progression biomarker to complement CEA mRNA, for improved selection of patients in need of adjuvant therapy and intensified follow-up after surgery. METHODS: Lymph nodes of pTNM stage I-IV CRC-(166 patients/503 lymph nodes) and control (23/108) patients were collected at surgery and analysed by quantitative RT-PCR. RESULTS: Lymph node KLK6 positivity was an indicator of poor outcome (hazard ratio 3.7). Risk of recurrence and cancer death increased with KLK6 lymph node levels. Patients with KLK6 lymph node levels above the 90th percentile had a hazard ratio of 6.5 and 76 months shorter average survival time compared to patients with KLK6 negative nodes. The KLK6 positivity in lymph nodes with few tumour cells, that is, low CEA mRNA levels, also indicated poor prognosis (hazard ratio 2.8). CONCLUSION: In CRC patients, lymph node KLK6 positivity indicated presence of aggressive tumour cells associated with poor prognosis and high risk of tumour recurrence. British Journal of Cancer (2012) 107, 150-157. doi: 10.1038/bjc.2012.220 www.bjcancer.com Published online 14 June 2012 (C) 2012 Cancer Research UK (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
colorectal neoplasms, lymphatic metastasis, neoplasm staging, prognosis, biomarker mRNA, quantitative RT-PCR
in
British Journal of Cancer
volume
107
issue
1
pages
150 - 157
publisher
Nature Publishing Group
external identifiers
  • wos:000305888400022
  • scopus:84863003678
ISSN
1532-1827
DOI
10.1038/bjc.2012.220
language
English
LU publication?
yes
id
bd1a5f27-6e47-4900-a1fd-29108a6867d6 (old id 3001677)
date added to LUP
2012-09-03 07:20:34
date last changed
2017-05-14 03:16:45
@article{bd1a5f27-6e47-4900-a1fd-29108a6867d6,
  abstract     = {BACKGROUND: A most important characteristic feature for poor prognosis in colorectal cancer (CRC) is the presence of lymph node metastasis. Determination of carcinoembryonic antigen (CEA) mRNA levels in lymph nodes has proven powerful for quantification of disseminated tumour cells. Here, we investigate the utility of human tissue kallikrein-related peptidase 6 (KLK6) mRNA as a progression biomarker to complement CEA mRNA, for improved selection of patients in need of adjuvant therapy and intensified follow-up after surgery. METHODS: Lymph nodes of pTNM stage I-IV CRC-(166 patients/503 lymph nodes) and control (23/108) patients were collected at surgery and analysed by quantitative RT-PCR. RESULTS: Lymph node KLK6 positivity was an indicator of poor outcome (hazard ratio 3.7). Risk of recurrence and cancer death increased with KLK6 lymph node levels. Patients with KLK6 lymph node levels above the 90th percentile had a hazard ratio of 6.5 and 76 months shorter average survival time compared to patients with KLK6 negative nodes. The KLK6 positivity in lymph nodes with few tumour cells, that is, low CEA mRNA levels, also indicated poor prognosis (hazard ratio 2.8). CONCLUSION: In CRC patients, lymph node KLK6 positivity indicated presence of aggressive tumour cells associated with poor prognosis and high risk of tumour recurrence. British Journal of Cancer (2012) 107, 150-157. doi: 10.1038/bjc.2012.220 www.bjcancer.com Published online 14 June 2012 (C) 2012 Cancer Research UK},
  author       = {Ohlsson, L. and Lindmark, Gudrun and Israelsson, A. and Palmqvist, R. and Oberg, A. and Hammarstrom, M-L and Hammarstrom, S.},
  issn         = {1532-1827},
  keyword      = {colorectal neoplasms,lymphatic metastasis,neoplasm staging,prognosis,biomarker mRNA,quantitative RT-PCR},
  language     = {eng},
  number       = {1},
  pages        = {150--157},
  publisher    = {Nature Publishing Group},
  series       = {British Journal of Cancer},
  title        = {Lymph node tissue kallikrein-related peptidase 6 mRNA: a progression marker for colorectal cancer},
  url          = {http://dx.doi.org/10.1038/bjc.2012.220},
  volume       = {107},
  year         = {2012},
}