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Hydrodynamic trapping of molecules in lipid bilayers

Joensson, Peter; McColl, James; Clarke, Richard W.; Ostanin, Victor P.; Jönsson, Bengt LU and Klenerman, David (2012) In Proceedings of the National Academy of Sciences 109(26). p.10328-10333
Abstract
In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the... (More)
In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the hydrodynamic forces affects the accumulation of protein molecules in the trap. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
intermolecular forces, membrane proteins, mobility, non-contact, tweezers, ion conductance microscopy
in
Proceedings of the National Academy of Sciences
volume
109
issue
26
pages
10328 - 10333
publisher
National Acad Sciences
external identifiers
  • wos:000306291400052
  • scopus:84862998686
ISSN
1091-6490
DOI
10.1073/pnas.1202858109
language
English
LU publication?
yes
id
a368ebbd-01f9-45be-9068-5afacea52e84 (old id 3001700)
date added to LUP
2012-08-21 10:38:28
date last changed
2017-11-19 03:12:53
@article{a368ebbd-01f9-45be-9068-5afacea52e84,
  abstract     = {In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the hydrodynamic forces affects the accumulation of protein molecules in the trap.},
  author       = {Joensson, Peter and McColl, James and Clarke, Richard W. and Ostanin, Victor P. and Jönsson, Bengt and Klenerman, David},
  issn         = {1091-6490},
  keyword      = {intermolecular forces,membrane proteins,mobility,non-contact,tweezers,ion conductance microscopy},
  language     = {eng},
  number       = {26},
  pages        = {10328--10333},
  publisher    = {National Acad Sciences},
  series       = {Proceedings of the National Academy of Sciences},
  title        = {Hydrodynamic trapping of molecules in lipid bilayers},
  url          = {http://dx.doi.org/10.1073/pnas.1202858109},
  volume       = {109},
  year         = {2012},
}