Hydrodynamic trapping of molecules in lipid bilayers
(2012) In Proceedings of the National Academy of Sciences 109(26). p.10328-10333- Abstract
- In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the... (More)
- In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the hydrodynamic forces affects the accumulation of protein molecules in the trap. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3001700
- author
- Joensson, Peter LU ; McColl, James ; Clarke, Richard W. ; Ostanin, Victor P. ; Jönsson, Bengt LU and Klenerman, David
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- intermolecular forces, membrane proteins, mobility, non-contact, tweezers, ion conductance microscopy
- in
- Proceedings of the National Academy of Sciences
- volume
- 109
- issue
- 26
- pages
- 10328 - 10333
- publisher
- National Academy of Sciences
- external identifiers
-
- wos:000306291400052
- scopus:84862998686
- pmid:22699491
- ISSN
- 1091-6490
- DOI
- 10.1073/pnas.1202858109
- language
- English
- LU publication?
- yes
- id
- a368ebbd-01f9-45be-9068-5afacea52e84 (old id 3001700)
- date added to LUP
- 2016-04-01 10:33:49
- date last changed
- 2022-03-19 22:01:09
@article{a368ebbd-01f9-45be-9068-5afacea52e84, abstract = {{In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the hydrodynamic forces affects the accumulation of protein molecules in the trap.}}, author = {{Joensson, Peter and McColl, James and Clarke, Richard W. and Ostanin, Victor P. and Jönsson, Bengt and Klenerman, David}}, issn = {{1091-6490}}, keywords = {{intermolecular forces; membrane proteins; mobility; non-contact; tweezers; ion conductance microscopy}}, language = {{eng}}, number = {{26}}, pages = {{10328--10333}}, publisher = {{National Academy of Sciences}}, series = {{Proceedings of the National Academy of Sciences}}, title = {{Hydrodynamic trapping of molecules in lipid bilayers}}, url = {{http://dx.doi.org/10.1073/pnas.1202858109}}, doi = {{10.1073/pnas.1202858109}}, volume = {{109}}, year = {{2012}}, }