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Importance of obtaining independent measures of insulin secretion and insulin sensitivity during the same test - Results with the Botnia clamp

Tripathy, Devjit LU ; Wessman, Y; Gullstrom, M; Tuomi, T and Groop, Leif LU (2003) In Diabetes Care 26(5). p.1395-1401
Abstract
OBJECTIVE - To validate and apply a method for independent assessment of insulin secretion and insulin sensitivity (S-l) during file same test that is, an intravenous glucose tolerance test followed by a euglycemic-hyperinsulinemic clamp, also called the Botnia clamp. This test was then applied to nondiabetic subjects with (FH+)and without (FH-) a first-degree family history of diabetes. RESEARCH DESIGN AND METHODS - The Botnia clamp measures the first-phase insulin response (FPIR) to 0.3g/k, glucose i.v. and insulin sensitivity (NI-value) front a 2-h euglycemic clamp begun 60 ruin alter the glucose bolus. The W-value obtained during the Botnia clamp was compared With M-values obtained during a regular euglycemic clamp without prior... (More)
OBJECTIVE - To validate and apply a method for independent assessment of insulin secretion and insulin sensitivity (S-l) during file same test that is, an intravenous glucose tolerance test followed by a euglycemic-hyperinsulinemic clamp, also called the Botnia clamp. This test was then applied to nondiabetic subjects with (FH+)and without (FH-) a first-degree family history of diabetes. RESEARCH DESIGN AND METHODS - The Botnia clamp measures the first-phase insulin response (FPIR) to 0.3g/k, glucose i.v. and insulin sensitivity (NI-value) front a 2-h euglycemic clamp begun 60 ruin alter the glucose bolus. The W-value obtained during the Botnia clamp was compared With M-values obtained during a regular euglycemic clamp without prior glucose bolus. Repeated tests were performed in random order in subjects with normal and abnormal glucose tolerance, Finally, the test was applied to subjects with and without a family history of type 2 diabetes. RESULTS - S-l and insulin secretion from this test showed a high degree of reproducibility, and the M-value obtained with the Botnia clamp correlated strongly,with the M-value from a euglycemic clamp without prior glucose bolus (r - 0.953, P < 0.005). FH+ subjects showed decreased S-l (P = 0.02), hut similar FPIR, compared with FH- subjects, However, insulin Secretion adjusted for file degree of insulin resistance was significantly impaired (P = 0.04). CONCLUSIONS - In conclusion, the Botnia clamp provides reliable and independent measures of S-l and beta-cell function during, the same test. As illustrated above, knowledge of the degree of S-l is mandatory When presenting data on insulin secretion. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diabetes Care
volume
26
issue
5
pages
1395 - 1401
publisher
American Diabetes Association
external identifiers
  • pmid:12716795
  • wos:000185505400012
  • scopus:0042168834
ISSN
1935-5548
language
English
LU publication?
yes
id
b668e29b-7ec2-4b10-b0c1-4418fcea5995 (old id 300587)
alternative location
http://care.diabetesjournals.org/cgi/content/abstract/26/5/1395
date added to LUP
2007-09-23 12:04:20
date last changed
2018-05-29 09:20:07
@article{b668e29b-7ec2-4b10-b0c1-4418fcea5995,
  abstract     = {OBJECTIVE - To validate and apply a method for independent assessment of insulin secretion and insulin sensitivity (S-l) during file same test that is, an intravenous glucose tolerance test followed by a euglycemic-hyperinsulinemic clamp, also called the Botnia clamp. This test was then applied to nondiabetic subjects with (FH+)and without (FH-) a first-degree family history of diabetes. RESEARCH DESIGN AND METHODS - The Botnia clamp measures the first-phase insulin response (FPIR) to 0.3g/k, glucose i.v. and insulin sensitivity (NI-value) front a 2-h euglycemic clamp begun 60 ruin alter the glucose bolus. The W-value obtained during the Botnia clamp was compared With M-values obtained during a regular euglycemic clamp without prior glucose bolus. Repeated tests were performed in random order in subjects with normal and abnormal glucose tolerance, Finally, the test was applied to subjects with and without a family history of type 2 diabetes. RESULTS - S-l and insulin secretion from this test showed a high degree of reproducibility, and the M-value obtained with the Botnia clamp correlated strongly,with the M-value from a euglycemic clamp without prior glucose bolus (r - 0.953, P &lt; 0.005). FH+ subjects showed decreased S-l (P = 0.02), hut similar FPIR, compared with FH- subjects, However, insulin Secretion adjusted for file degree of insulin resistance was significantly impaired (P = 0.04). CONCLUSIONS - In conclusion, the Botnia clamp provides reliable and independent measures of S-l and beta-cell function during, the same test. As illustrated above, knowledge of the degree of S-l is mandatory When presenting data on insulin secretion.},
  author       = {Tripathy, Devjit and Wessman, Y and Gullstrom, M and Tuomi, T and Groop, Leif},
  issn         = {1935-5548},
  language     = {eng},
  number       = {5},
  pages        = {1395--1401},
  publisher    = {American Diabetes Association},
  series       = {Diabetes Care},
  title        = {Importance of obtaining independent measures of insulin secretion and insulin sensitivity during the same test - Results with the Botnia clamp},
  volume       = {26},
  year         = {2003},
}