The MAP kinases are differently utilized by CD28 and CD2 adhesion pathways in superantigen-activated Jurkat T cells
(2003) In Biological Research 36(2). p.263-278- Abstract
- To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and... (More)
- To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation resulted in the up-regulation of IL-4 and IFN-gamma promoters, IL-2 promoter activity and production of IL-2 were only seen after B7-1 costimulation. However, contrary to what has been previously proposed, we show that costimulation with either B7-1 or LFA-3 further enhanced the ERK-2 activity and strongly activated the p38 MAPK pathway, but only B7-1 costiniulation induced high levels of JNK-1 activity. These data Suggest that the differential effect of CD28 vs. CD2 can be related to the difference in the ability of the two pathways to induce JNK-1 activity. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/301447
- author
- Visse, Edward LU ; Inostroza, J ; Cabello, G and Parra, E
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- c-Jun N-terminal kinase, signal regulated kinase, extracellular, CD28 response element, staphylococcal enterotoxin A-E, interleukin-2
- in
- Biological Research
- volume
- 36
- issue
- 2
- pages
- 263 - 278
- publisher
- Sociedad de Biología de Chile
- external identifiers
-
- wos:000185322300017
- pmid:14513721
- scopus:0141717716
- ISSN
- 0717-6287
- language
- English
- LU publication?
- yes
- id
- 9fa29c45-523a-475e-a9f1-3c9c0c51c46a (old id 301447)
- alternative location
- http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200016&lng=en&nrm=iso
- date added to LUP
- 2016-04-01 12:19:56
- date last changed
- 2022-01-27 02:07:21
@article{9fa29c45-523a-475e-a9f1-3c9c0c51c46a, abstract = {{To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation resulted in the up-regulation of IL-4 and IFN-gamma promoters, IL-2 promoter activity and production of IL-2 were only seen after B7-1 costimulation. However, contrary to what has been previously proposed, we show that costimulation with either B7-1 or LFA-3 further enhanced the ERK-2 activity and strongly activated the p38 MAPK pathway, but only B7-1 costiniulation induced high levels of JNK-1 activity. These data Suggest that the differential effect of CD28 vs. CD2 can be related to the difference in the ability of the two pathways to induce JNK-1 activity.}}, author = {{Visse, Edward and Inostroza, J and Cabello, G and Parra, E}}, issn = {{0717-6287}}, keywords = {{c-Jun N-terminal kinase; signal regulated kinase; extracellular; CD28 response element; staphylococcal enterotoxin A-E; interleukin-2}}, language = {{eng}}, number = {{2}}, pages = {{263--278}}, publisher = {{Sociedad de Biología de Chile}}, series = {{Biological Research}}, title = {{The MAP kinases are differently utilized by CD28 and CD2 adhesion pathways in superantigen-activated Jurkat T cells}}, url = {{http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200016&lng=en&nrm=iso}}, volume = {{36}}, year = {{2003}}, }