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The MAP kinases are differently utilized by CD28 and CD2 adhesion pathways in superantigen-activated Jurkat T cells

Visse, Edward LU ; Inostroza, J; Cabello, G and Parra, E (2003) In Biological Research 36(2). p.263-278
Abstract
To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and... (More)
To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation resulted in the up-regulation of IL-4 and IFN-gamma promoters, IL-2 promoter activity and production of IL-2 were only seen after B7-1 costimulation. However, contrary to what has been previously proposed, we show that costimulation with either B7-1 or LFA-3 further enhanced the ERK-2 activity and strongly activated the p38 MAPK pathway, but only B7-1 costiniulation induced high levels of JNK-1 activity. These data Suggest that the differential effect of CD28 vs. CD2 can be related to the difference in the ability of the two pathways to induce JNK-1 activity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
c-Jun N-terminal kinase, signal regulated kinase, extracellular, CD28 response element, staphylococcal enterotoxin A-E, interleukin-2
in
Biological Research
volume
36
issue
2
pages
263 - 278
publisher
Sociedad de Biología de Chile
external identifiers
  • wos:000185322300017
  • pmid:14513721
  • scopus:0141717716
ISSN
0717-6287
language
English
LU publication?
yes
id
9fa29c45-523a-475e-a9f1-3c9c0c51c46a (old id 301447)
alternative location
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200016&lng=en&nrm=iso
date added to LUP
2007-09-23 14:00:58
date last changed
2018-01-07 06:04:38
@article{9fa29c45-523a-475e-a9f1-3c9c0c51c46a,
  abstract     = {To mimic the two-signal requirements for T cell activation mediated by ligands, we exposed the superantigens SEA or SEE (signal 1) to T cells incubated with HLA-DR/LFA-3 or HLA-DR/137-1-CHO transfected cells (signal 2). LFA-3 costimulation was able to induce T cell proliferation as well as IFN-gamma and IL-4 production at similar levels as in cells induced by B7-1. Analysis of the CD28RE of the IL-2 promoter showed specific transcription factor recruitment at the CD28RE element upon induction by B7-1/SEE. Further functional Studies with an IL-2 enhancer-promoter carrying either wild type or mutated versions of the CD28RE site revealed that this element is necessary for full activation upon B7-1 costimulation. While both CD28/B7-1 and CD2/LFA-3 costimulation resulted in the up-regulation of IL-4 and IFN-gamma promoters, IL-2 promoter activity and production of IL-2 were only seen after B7-1 costimulation. However, contrary to what has been previously proposed, we show that costimulation with either B7-1 or LFA-3 further enhanced the ERK-2 activity and strongly activated the p38 MAPK pathway, but only B7-1 costiniulation induced high levels of JNK-1 activity. These data Suggest that the differential effect of CD28 vs. CD2 can be related to the difference in the ability of the two pathways to induce JNK-1 activity.},
  author       = {Visse, Edward and Inostroza, J and Cabello, G and Parra, E},
  issn         = {0717-6287},
  keyword      = {c-Jun N-terminal kinase,signal regulated kinase,extracellular,CD28 response element,staphylococcal enterotoxin A-E,interleukin-2},
  language     = {eng},
  number       = {2},
  pages        = {263--278},
  publisher    = {Sociedad de Biología de Chile},
  series       = {Biological Research},
  title        = {The MAP kinases are differently utilized by CD28 and CD2 adhesion pathways in superantigen-activated Jurkat T cells},
  volume       = {36},
  year         = {2003},
}