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Calcitonin gene-related peptide and substance P induce pronounced motor effects in the female rat urethra in vivo

Radziszewski, P; Soller, Wolfgang LU and Mattiasson, Anders LU (2003) In Scandinavian Journal of Urology and Nephrology1967-01-01+01:002013-01-01+01:00 37(4). p.275-280
Abstract
Objective: This study was performed to investigate the influence of calcitonin gene-related peptide (CGRP) and substance P (SP) on the intra-urethral pressure in female rats, as both these neuropeptides have been demonstrated to occur in nerve fibres throughout all layers of the intrinsic external urethral sphincter of the rat. Material and Methods: Both CGRP and SP were administered intra-arterially relatively close to the bladder in ketamine-anaesthetized female rats. The maximum urethral pressure (MUP) was recorded continuously using a 4 F microtip single-transducer catheter. Results: Pronounced effects on the intra-urethral pressure were found with both CGRP and SP. CGRP at the maximum dose given (10 mug) induced an immediate,... (More)
Objective: This study was performed to investigate the influence of calcitonin gene-related peptide (CGRP) and substance P (SP) on the intra-urethral pressure in female rats, as both these neuropeptides have been demonstrated to occur in nerve fibres throughout all layers of the intrinsic external urethral sphincter of the rat. Material and Methods: Both CGRP and SP were administered intra-arterially relatively close to the bladder in ketamine-anaesthetized female rats. The maximum urethral pressure (MUP) was recorded continuously using a 4 F microtip single-transducer catheter. Results: Pronounced effects on the intra-urethral pressure were found with both CGRP and SP. CGRP at the maximum dose given (10 mug) induced an immediate, pronounced, long-lasting decrease in pressure from 28 +/- 4 to 10 +/- 2 cmH 2 O, amounting to 65% of the MUP. SP (10 mug) instead induced a forceful, phasic, peak-like contractile response with a 170% increase in urethral pressure from 33 +/- 6 to 87 +/- 6 cmH 2 O of the initial control level of MUP. Antagonists to CGRP and SP did not induce any pressure changes per se. Conclusion: These results indicate that both CGRP and SP are of importance for the peripheral motor regulation of the external urethral sphincter, and hence possibly also of physiological importance for lower urinary tract function during essential parts of the micturition cycle. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
urethral relaxation, rat, calcitonin gene-related peptide, neuropeptides, substance P
in
Scandinavian Journal of Urology and Nephrology1967-01-01+01:002013-01-01+01:00
volume
37
issue
4
pages
275 - 280
publisher
Taylor & Francis
external identifiers
  • pmid:12944183
  • wos:000184982200001
  • scopus:0141728365
ISSN
0036-5599
DOI
10.1080/00365590310004761
language
English
LU publication?
yes
id
d116d397-90c8-4c59-b38c-a6ef14819681 (old id 301808)
date added to LUP
2007-09-20 12:08:17
date last changed
2018-05-29 11:37:38
@article{d116d397-90c8-4c59-b38c-a6ef14819681,
  abstract     = {Objective: This study was performed to investigate the influence of calcitonin gene-related peptide (CGRP) and substance P (SP) on the intra-urethral pressure in female rats, as both these neuropeptides have been demonstrated to occur in nerve fibres throughout all layers of the intrinsic external urethral sphincter of the rat. Material and Methods: Both CGRP and SP were administered intra-arterially relatively close to the bladder in ketamine-anaesthetized female rats. The maximum urethral pressure (MUP) was recorded continuously using a 4 F microtip single-transducer catheter. Results: Pronounced effects on the intra-urethral pressure were found with both CGRP and SP. CGRP at the maximum dose given (10 mug) induced an immediate, pronounced, long-lasting decrease in pressure from 28 +/- 4 to 10 +/- 2 cmH 2 O, amounting to 65% of the MUP. SP (10 mug) instead induced a forceful, phasic, peak-like contractile response with a 170% increase in urethral pressure from 33 +/- 6 to 87 +/- 6 cmH 2 O of the initial control level of MUP. Antagonists to CGRP and SP did not induce any pressure changes per se. Conclusion: These results indicate that both CGRP and SP are of importance for the peripheral motor regulation of the external urethral sphincter, and hence possibly also of physiological importance for lower urinary tract function during essential parts of the micturition cycle.},
  author       = {Radziszewski, P and Soller, Wolfgang and Mattiasson, Anders},
  issn         = {0036-5599},
  keyword      = {urethral relaxation,rat,calcitonin gene-related peptide,neuropeptides,substance P},
  language     = {eng},
  number       = {4},
  pages        = {275--280},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Urology and Nephrology1967-01-01+01:002013-01-01+01:00},
  title        = {Calcitonin gene-related peptide and substance P induce pronounced motor effects in the female rat urethra in vivo},
  url          = {http://dx.doi.org/10.1080/00365590310004761},
  volume       = {37},
  year         = {2003},
}