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Direct assessment of microbial colonisation in damp houses by chemical marker analysis

Szponar, B; Szponar, A and Larsson, Lennart LU (2003) In Indoor + Built Environment 12(4). p.251-254
Abstract
Ergosterol was used as a marker of fungal biomass and 3-hydroxy fatty acids were used as markers of bacterial endotoxin to determine microbial colonisation of building materials by gas chromatography-mass spectrometry. Pieces (0.3-0.5 g) of materials from two water-damaged buildings were hydrolysed to liberate the markers from larger microbial structures; the preparations were then purified, derivatised, and analysed. The marker patterns obtained revealed that the microbial communities differed between the two buildings both qualitatively and quantitatively. The chemical-analytical method described should be useful for detecting and characterising microbial colonisation on surfaces in, for example, damp buildings.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
fungi, bacteria, ergosterol, 3-hydroxy fatty acids, building materials
in
Indoor + Built Environment
volume
12
issue
4
pages
251 - 254
publisher
SAGE Publications Inc.
external identifiers
  • wos:000184949300012
  • scopus:0041466165
ISSN
1420-326X
DOI
10.1177/1420326X03035164
language
English
LU publication?
yes
id
3bef6abb-5566-4759-880e-30954df28cea (old id 303530)
date added to LUP
2007-09-23 10:00:36
date last changed
2018-01-07 08:55:32
@article{3bef6abb-5566-4759-880e-30954df28cea,
  abstract     = {Ergosterol was used as a marker of fungal biomass and 3-hydroxy fatty acids were used as markers of bacterial endotoxin to determine microbial colonisation of building materials by gas chromatography-mass spectrometry. Pieces (0.3-0.5 g) of materials from two water-damaged buildings were hydrolysed to liberate the markers from larger microbial structures; the preparations were then purified, derivatised, and analysed. The marker patterns obtained revealed that the microbial communities differed between the two buildings both qualitatively and quantitatively. The chemical-analytical method described should be useful for detecting and characterising microbial colonisation on surfaces in, for example, damp buildings.},
  author       = {Szponar, B and Szponar, A and Larsson, Lennart},
  issn         = {1420-326X},
  keyword      = {fungi,bacteria,ergosterol,3-hydroxy fatty acids,building materials},
  language     = {eng},
  number       = {4},
  pages        = {251--254},
  publisher    = {SAGE Publications Inc.},
  series       = {Indoor + Built Environment},
  title        = {Direct assessment of microbial colonisation in damp houses by chemical marker analysis},
  url          = {http://dx.doi.org/10.1177/1420326X03035164},
  volume       = {12},
  year         = {2003},
}