Advanced

Glycerol dehydrogenase based amperometric biosensor for monitoring of glycerol in alcoholic beverages

Niculescu, M; Sigina, S and Csöregi, Elisabeth LU (2003) In Analytical Letters 36(9). p.1721-1737
Abstract
Biosensors for measurement of glycerol in FIA were constructed using NAD(+)-dependent glycerol dehydrogenase (GlDH) either co-immobilized with phenazine methosulphate (PMS) or cross-linked to an Os-complex-modified poly(vinylimidazole) redox polymer (PVI(13)dmeOs) using poly(ethyleneglycole) diglycidilether (PEGDGE). The GlDH/PMS sensor was characterized by a linear range of 0.01-1mM glycerol, a sensitivity of 1.83mA/Mcm(2), a detection limit (calculated as three times the signal-to-noise ratio) of 0.9muM, and with 50% residual activity kept after 15h of continuous operation at a sample throughput of 30 injections/h. The redox hydrogel-based biosensors showed the same dynamic range, but improved biosensors characteristics, i.e., a... (More)
Biosensors for measurement of glycerol in FIA were constructed using NAD(+)-dependent glycerol dehydrogenase (GlDH) either co-immobilized with phenazine methosulphate (PMS) or cross-linked to an Os-complex-modified poly(vinylimidazole) redox polymer (PVI(13)dmeOs) using poly(ethyleneglycole) diglycidilether (PEGDGE). The GlDH/PMS sensor was characterized by a linear range of 0.01-1mM glycerol, a sensitivity of 1.83mA/Mcm(2), a detection limit (calculated as three times the signal-to-noise ratio) of 0.9muM, and with 50% residual activity kept after 15h of continuous operation at a sample throughput of 30 injections/h. The redox hydrogel-based biosensors showed the same dynamic range, but improved biosensors characteristics, i.e., a sensitivity of 4.79 mA/Mcm, a detection limit of 0.1 muM, and a signal loss of only 20% after 15h of operation in the same conditions. The optimized biosensor configurations were further used for analysis of glycerol in wine and the obtained results were compared with the ones obtained by spectrophotometrical experiments. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
glycerol biosensor, flow injection, wine analysis
in
Analytical Letters
volume
36
issue
9
pages
1721 - 1737
publisher
Taylor & Francis
external identifiers
  • wos:000184660600005
  • scopus:0043063957
ISSN
0003-2719
DOI
10.1081/AL-120023611
language
English
LU publication?
yes
id
d0bd3d3e-39d7-4ff8-8414-d6b625dafbb7 (old id 304082)
date added to LUP
2007-09-18 15:35:20
date last changed
2018-01-07 09:28:33
@article{d0bd3d3e-39d7-4ff8-8414-d6b625dafbb7,
  abstract     = {Biosensors for measurement of glycerol in FIA were constructed using NAD(+)-dependent glycerol dehydrogenase (GlDH) either co-immobilized with phenazine methosulphate (PMS) or cross-linked to an Os-complex-modified poly(vinylimidazole) redox polymer (PVI(13)dmeOs) using poly(ethyleneglycole) diglycidilether (PEGDGE). The GlDH/PMS sensor was characterized by a linear range of 0.01-1mM glycerol, a sensitivity of 1.83mA/Mcm(2), a detection limit (calculated as three times the signal-to-noise ratio) of 0.9muM, and with 50% residual activity kept after 15h of continuous operation at a sample throughput of 30 injections/h. The redox hydrogel-based biosensors showed the same dynamic range, but improved biosensors characteristics, i.e., a sensitivity of 4.79 mA/Mcm, a detection limit of 0.1 muM, and a signal loss of only 20% after 15h of operation in the same conditions. The optimized biosensor configurations were further used for analysis of glycerol in wine and the obtained results were compared with the ones obtained by spectrophotometrical experiments.},
  author       = {Niculescu, M and Sigina, S and Csöregi, Elisabeth},
  issn         = {0003-2719},
  keyword      = {glycerol biosensor,flow injection,wine analysis},
  language     = {eng},
  number       = {9},
  pages        = {1721--1737},
  publisher    = {Taylor & Francis},
  series       = {Analytical Letters},
  title        = {Glycerol dehydrogenase based amperometric biosensor for monitoring of glycerol in alcoholic beverages},
  url          = {http://dx.doi.org/10.1081/AL-120023611},
  volume       = {36},
  year         = {2003},
}