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Insulin granule dynamics in pancreatic beta cells

Rorsman, Patrik LU and Renström, Erik LU (2003) In Diabetologia 46(8). p.1029-1045
Abstract
Glucose-induced insulin secretion in response to a step increase in blood glucose concentrations follows a biphasic time course consisting of a rapid and transient first phase followed by a slowly developing and sustained second phase. Because Type 2 diabetes involves defects of insulin secretion, manifested as a loss of first phase and a reduction of second phase, it is important to understand the cellular mechanisms underlying biphasic insulin secretion. Insulin release involves the packaging of insulin in small (diameter approximate to0.3 mum) secretory granules, the trafficking of these granules to the plasma membrane, the exocytotic fusion of the granules with the plasma membrane and eventually the retrieval of the secreted membranes... (More)
Glucose-induced insulin secretion in response to a step increase in blood glucose concentrations follows a biphasic time course consisting of a rapid and transient first phase followed by a slowly developing and sustained second phase. Because Type 2 diabetes involves defects of insulin secretion, manifested as a loss of first phase and a reduction of second phase, it is important to understand the cellular mechanisms underlying biphasic insulin secretion. Insulin release involves the packaging of insulin in small (diameter approximate to0.3 mum) secretory granules, the trafficking of these granules to the plasma membrane, the exocytotic fusion of the granules with the plasma membrane and eventually the retrieval of the secreted membranes by endocytosis. Until recently, studies on insulin secretion have been confined to the appearance of insulin in the extracellular space and the cellular events preceding exocytosis have been inaccessible to more detailed analysis. Evidence from a variety of secretory tissues, including pancreatic islet cells suggests, however, that the secretory granules can be functionally divided into distinct pools that are distinguished by their release competence and/or proximity to the plasma membrane. The introduction of fluorescent proteins that can be targeted to the secretory granules, in combination with the advent of new techniques that allow real-time imaging of granule trafficking in living cells (granule dynamics), has led to an explosion of our knowledge of the pre-exocytotic and post-exocytotic processes in the beta cell. Here we discuss these observations in relation to previous functional and ultra-structural data as well as the secretory defects of Type 2 diabetes. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
confocal microscopy, diabetes, exocytosis, secretory granules, pancreatic islets, insulin
in
Diabetologia
volume
46
issue
8
pages
1029 - 1045
publisher
Springer Verlag
external identifiers
  • pmid:12879249
  • wos:000184641000001
  • scopus:0042879951
ISSN
1432-0428
DOI
10.1007/s00125-003-1153-1
language
English
LU publication?
yes
id
6e5b314b-de9c-49a1-aa70-96c407e95ed3 (old id 304682)
date added to LUP
2007-09-20 18:19:37
date last changed
2018-10-07 03:41:57
@article{6e5b314b-de9c-49a1-aa70-96c407e95ed3,
  abstract     = {Glucose-induced insulin secretion in response to a step increase in blood glucose concentrations follows a biphasic time course consisting of a rapid and transient first phase followed by a slowly developing and sustained second phase. Because Type 2 diabetes involves defects of insulin secretion, manifested as a loss of first phase and a reduction of second phase, it is important to understand the cellular mechanisms underlying biphasic insulin secretion. Insulin release involves the packaging of insulin in small (diameter approximate to0.3 mum) secretory granules, the trafficking of these granules to the plasma membrane, the exocytotic fusion of the granules with the plasma membrane and eventually the retrieval of the secreted membranes by endocytosis. Until recently, studies on insulin secretion have been confined to the appearance of insulin in the extracellular space and the cellular events preceding exocytosis have been inaccessible to more detailed analysis. Evidence from a variety of secretory tissues, including pancreatic islet cells suggests, however, that the secretory granules can be functionally divided into distinct pools that are distinguished by their release competence and/or proximity to the plasma membrane. The introduction of fluorescent proteins that can be targeted to the secretory granules, in combination with the advent of new techniques that allow real-time imaging of granule trafficking in living cells (granule dynamics), has led to an explosion of our knowledge of the pre-exocytotic and post-exocytotic processes in the beta cell. Here we discuss these observations in relation to previous functional and ultra-structural data as well as the secretory defects of Type 2 diabetes.},
  author       = {Rorsman, Patrik and Renström, Erik},
  issn         = {1432-0428},
  keyword      = {confocal microscopy,diabetes,exocytosis,secretory granules,pancreatic islets,insulin},
  language     = {eng},
  number       = {8},
  pages        = {1029--1045},
  publisher    = {Springer Verlag},
  series       = {Diabetologia},
  title        = {Insulin granule dynamics in pancreatic beta cells},
  url          = {http://dx.doi.org/10.1007/s00125-003-1153-1},
  volume       = {46},
  year         = {2003},
}