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Molecular identification of bloodmeals and species composition in Culicoides biting midges.

Videvall, Elin LU ; Bensch, Staffan LU ; Ander, M; Chirico, J; Sigvald, R and Ignell, R (2012) In Medical and Veterinary Entomology
Abstract
Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between... (More)
Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between mammalophilic and ornithophilic Culicoides species. Host sequences found matches in horse (n = 59), sheep (n = 39), cattle (n = 26), Eurasian elk (n = 1) and 10 different bird species (n = 18). We identified 15 Culicoides species previously recorded in Scandinavia and four additional species haplotypes that were distinctly different from the described species. All ornithophilic individuals (n = 23) were caught exclusively in the suction traps, as were, interestingly, almost all mammalophilic species (n = 41), indicating that many biting midge species may be able to cover long distances after completing a bloodmeal. These results add new information on the composition of Culicoides species and their host preferences and their potential long-distance dispersal while blood-engorged. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Medical and Veterinary Entomology
publisher
The Royal Entomological Society
external identifiers
  • wos:000315033000012
  • pmid:22882691
  • scopus:84873986490
ISSN
0269-283X
DOI
10.1111/j.1365-2915.2012.01038.x
language
English
LU publication?
yes
id
a18aff34-13a9-4129-ae18-8ce185fa7285 (old id 3047607)
date added to LUP
2012-09-10 15:26:49
date last changed
2017-08-27 05:47:45
@article{a18aff34-13a9-4129-ae18-8ce185fa7285,
  abstract     = {Investigations of host preferences in haematophagous insects, including Culicoides biting midges (Diptera: Ceratopogonidae), are critical in order to assess transmission routes of vector-borne diseases. In this study, we collected and morphologically identified 164 blood-engorged Culicoides females caught in both light traps and permanent 12-m high suction traps during 2008-2010 in Sweden. Molecular analysis of the mitochondrial cytochrome c oxidase subunit I (COI) gene in the biting midges was performed to verify species classification, discern phylogenetic relationships and uncover possible cryptic species. Bloodmeal analysis using universal vertebrate cytochrome b primers revealed a clear distinction in host selection between mammalophilic and ornithophilic Culicoides species. Host sequences found matches in horse (n = 59), sheep (n = 39), cattle (n = 26), Eurasian elk (n = 1) and 10 different bird species (n = 18). We identified 15 Culicoides species previously recorded in Scandinavia and four additional species haplotypes that were distinctly different from the described species. All ornithophilic individuals (n = 23) were caught exclusively in the suction traps, as were, interestingly, almost all mammalophilic species (n = 41), indicating that many biting midge species may be able to cover long distances after completing a bloodmeal. These results add new information on the composition of Culicoides species and their host preferences and their potential long-distance dispersal while blood-engorged.},
  author       = {Videvall, Elin and Bensch, Staffan and Ander, M and Chirico, J and Sigvald, R and Ignell, R},
  issn         = {0269-283X},
  language     = {eng},
  publisher    = {The Royal Entomological Society},
  series       = {Medical and Veterinary Entomology},
  title        = {Molecular identification of bloodmeals and species composition in Culicoides biting midges.},
  url          = {http://dx.doi.org/10.1111/j.1365-2915.2012.01038.x},
  year         = {2012},
}