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Jejunoileal bypass changes the duodenal cholecystokinin and somatostatin cell density

Ockander, L; Hedenbro, Jan LU ; Rehfeld, JF and Sjölund, Kristina LU (2003) In Obesity Surgery 13(4). p.584-590
Abstract
Background: In obese patients, jejunoileal bypass (JIB) has been used to induce weight reduction. Changes in the neuroendocrine system may be affected by the JIB-operation, because the proximal small intestinal mucosa has a rich supply of endocrine cells and peptidergic nerves. Materials and Methods: In 37 obese patients operated with JIB 1-30 years ago, small intestinal biopsies were taken at the duodeno-jejunal flexure, proximal to the anastomosis and from 5 unoperated obese persons and 20 normal weight patients. The tissue specimens were processed for immunocyto-chemical demonstration of cells/nerves containing: gastrin, cholecystokinin (CCK), secretin, gastric inhibitory peptide (GIP), motilin, somatostatin, serotonin, glicentine,... (More)
Background: In obese patients, jejunoileal bypass (JIB) has been used to induce weight reduction. Changes in the neuroendocrine system may be affected by the JIB-operation, because the proximal small intestinal mucosa has a rich supply of endocrine cells and peptidergic nerves. Materials and Methods: In 37 obese patients operated with JIB 1-30 years ago, small intestinal biopsies were taken at the duodeno-jejunal flexure, proximal to the anastomosis and from 5 unoperated obese persons and 20 normal weight patients. The tissue specimens were processed for immunocyto-chemical demonstration of cells/nerves containing: gastrin, cholecystokinin (CCK), secretin, gastric inhibitory peptide (GIP), motilin, somatostatin, serotonin, glicentine, peptide YY (PYY), neurotensin, vasoactive intestinal peptide (VIP), substance P, neuropeptide Y (NPY) and galanin. The number of different endocrine cell-types were counted per unit length of mucosa, and the density of the peptidergic nerves was assessed semiquantitatively according to a schematic scale. Results: JIB-patients had an increased density of CCK and somatostatin cells in the duodenal mucosa. The CCK cells displayed a changed reaction pattern, with a greater cell number reacting with an antiserum directed towards a non-amidated mid-sequence of procholecystokinin compared with the other groups. In obese unoperated patients, the density of PYY and secretin cells was decreased compared with the JIB-patients and the density of the GIP cells compared with both other groups. Conclusion: JIB induces an up-regulation of somatostatin and CCK precursor-containing cells in the duodenal mucosa. The time duration after the JIB did not seem to influence the results. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunocytochemistry, secretin, PYY, GIP, somatostatin, cholecystokinin, endocrine cells, jejunoileal bypass, morbid obesity, bariatric surgery, peptidergic nerves
in
Obesity Surgery
volume
13
issue
4
pages
584 - 590
publisher
Springer
external identifiers
  • wos:000184499700002
  • pmid:12935359
  • scopus:0043128892
ISSN
1708-0428
DOI
10.1381/096089203322190781
language
English
LU publication?
yes
id
9e0e5173-7d2f-4a4f-9598-d53fc459a1e6 (old id 304927)
date added to LUP
2007-09-19 15:06:44
date last changed
2018-10-03 10:48:21
@article{9e0e5173-7d2f-4a4f-9598-d53fc459a1e6,
  abstract     = {Background: In obese patients, jejunoileal bypass (JIB) has been used to induce weight reduction. Changes in the neuroendocrine system may be affected by the JIB-operation, because the proximal small intestinal mucosa has a rich supply of endocrine cells and peptidergic nerves. Materials and Methods: In 37 obese patients operated with JIB 1-30 years ago, small intestinal biopsies were taken at the duodeno-jejunal flexure, proximal to the anastomosis and from 5 unoperated obese persons and 20 normal weight patients. The tissue specimens were processed for immunocyto-chemical demonstration of cells/nerves containing: gastrin, cholecystokinin (CCK), secretin, gastric inhibitory peptide (GIP), motilin, somatostatin, serotonin, glicentine, peptide YY (PYY), neurotensin, vasoactive intestinal peptide (VIP), substance P, neuropeptide Y (NPY) and galanin. The number of different endocrine cell-types were counted per unit length of mucosa, and the density of the peptidergic nerves was assessed semiquantitatively according to a schematic scale. Results: JIB-patients had an increased density of CCK and somatostatin cells in the duodenal mucosa. The CCK cells displayed a changed reaction pattern, with a greater cell number reacting with an antiserum directed towards a non-amidated mid-sequence of procholecystokinin compared with the other groups. In obese unoperated patients, the density of PYY and secretin cells was decreased compared with the JIB-patients and the density of the GIP cells compared with both other groups. Conclusion: JIB induces an up-regulation of somatostatin and CCK precursor-containing cells in the duodenal mucosa. The time duration after the JIB did not seem to influence the results.},
  author       = {Ockander, L and Hedenbro, Jan and Rehfeld, JF and Sjölund, Kristina},
  issn         = {1708-0428},
  keyword      = {immunocytochemistry,secretin,PYY,GIP,somatostatin,cholecystokinin,endocrine cells,jejunoileal bypass,morbid obesity,bariatric surgery,peptidergic nerves},
  language     = {eng},
  number       = {4},
  pages        = {584--590},
  publisher    = {Springer},
  series       = {Obesity Surgery},
  title        = {Jejunoileal bypass changes the duodenal cholecystokinin and somatostatin cell density},
  url          = {http://dx.doi.org/10.1381/096089203322190781},
  volume       = {13},
  year         = {2003},
}