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CO2 induced acute respiratory acidosis and brain tissue intracellular pH: a P-31 NMR study in swine

Martoft, L; Stodkilde-Jorgensen, H; Forslid, Anders LU ; Pedersen, HD and Jorgensen, PF (2003) In Laboratory Animals 37(3). p.241-248
Abstract
High concentration carbon dioxide (CO2) is used to promote pre-slaughter anaesthesia in swine and poultry, as well as short-lasting surgical anaesthesia and euthanasia in laboratory animals. Questions related to animal welfare have been raised, as CO2 anaesthesia does not set in momentarily. Carbon dioxide promotes anaesthesia by lowering the intracellular pH in the brain cells, but the dynamics of the changes in response to a high concentration of CO2 is not known. Based on P-31 NMR spectroscopy, we describe CO2-induced changes in intracellular pH in the brains of five pigs inhaling 90% CO2 in ambient air for a period of 60 s, and compare the results to changes in arterial blood pH, P-CO2, O-2 saturation and HCO(3)(-)concentration. The... (More)
High concentration carbon dioxide (CO2) is used to promote pre-slaughter anaesthesia in swine and poultry, as well as short-lasting surgical anaesthesia and euthanasia in laboratory animals. Questions related to animal welfare have been raised, as CO2 anaesthesia does not set in momentarily. Carbon dioxide promotes anaesthesia by lowering the intracellular pH in the brain cells, but the dynamics of the changes in response to a high concentration of CO2 is not known. Based on P-31 NMR spectroscopy, we describe CO2-induced changes in intracellular pH in the brains of five pigs inhaling 90% CO2 in ambient air for a period of 60 s, and compare the results to changes in arterial blood pH, P-CO2, O-2 saturation and HCO(3)(-)concentration. The intracellular pH paralleled the arterial pH and P-CO2 during inhalation of CO2; and it is suggested that the acute reaction to CO2 inhalation mainly reflects respiratory acidosis, and not metabolic regulation as for example transmembrane fluxes of H+/HCO3-. The intracellular pH decreased to approximately 6.7 within the 60 s inhalation period, and the situation was metabolically reversible after the end of CO2 inhalation. The fast decrease in intracellular pH supports the conclusion that high concentration CO2 leads to anaesthesia soon after the start of inhalation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
CNS, phosphate buffer, bicarbonate, carbon, blood gases, anaesthesia, dioxide inhalation
in
Laboratory Animals
volume
37
issue
3
pages
241 - 248
publisher
Royal Society of Medicine Press
external identifiers
  • wos:000184272900009
  • pmid:12869287
  • scopus:0037824536
ISSN
0023-6772
DOI
10.1258/002367703766453092
language
English
LU publication?
yes
id
7e412733-a83a-44fa-b530-0f42e6f3614b (old id 305207)
date added to LUP
2007-09-18 07:54:45
date last changed
2018-01-28 04:07:58
@article{7e412733-a83a-44fa-b530-0f42e6f3614b,
  abstract     = {High concentration carbon dioxide (CO2) is used to promote pre-slaughter anaesthesia in swine and poultry, as well as short-lasting surgical anaesthesia and euthanasia in laboratory animals. Questions related to animal welfare have been raised, as CO2 anaesthesia does not set in momentarily. Carbon dioxide promotes anaesthesia by lowering the intracellular pH in the brain cells, but the dynamics of the changes in response to a high concentration of CO2 is not known. Based on P-31 NMR spectroscopy, we describe CO2-induced changes in intracellular pH in the brains of five pigs inhaling 90% CO2 in ambient air for a period of 60 s, and compare the results to changes in arterial blood pH, P-CO2, O-2 saturation and HCO(3)(-)concentration. The intracellular pH paralleled the arterial pH and P-CO2 during inhalation of CO2; and it is suggested that the acute reaction to CO2 inhalation mainly reflects respiratory acidosis, and not metabolic regulation as for example transmembrane fluxes of H+/HCO3-. The intracellular pH decreased to approximately 6.7 within the 60 s inhalation period, and the situation was metabolically reversible after the end of CO2 inhalation. The fast decrease in intracellular pH supports the conclusion that high concentration CO2 leads to anaesthesia soon after the start of inhalation.},
  author       = {Martoft, L and Stodkilde-Jorgensen, H and Forslid, Anders and Pedersen, HD and Jorgensen, PF},
  issn         = {0023-6772},
  keyword      = {CNS,phosphate buffer,bicarbonate,carbon,blood gases,anaesthesia,dioxide inhalation},
  language     = {eng},
  number       = {3},
  pages        = {241--248},
  publisher    = {Royal Society of Medicine Press},
  series       = {Laboratory Animals},
  title        = {CO2 induced acute respiratory acidosis and brain tissue intracellular pH: a P-31 NMR study in swine},
  url          = {http://dx.doi.org/10.1258/002367703766453092},
  volume       = {37},
  year         = {2003},
}