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Nonrandom chromosomal aberrations and cytogenetic heterogeneity in gallbladder carcinomas

Gorunova, Ludmila LU ; Parada, Luis Antonio; Limon, J.; Jin, Yuesheng LU ; Hallén, Magnus LU ; Hagerstrand, Inga; Iliszko, M.; Wajda, Z. and Johansson, Bertil LU (1999) In Genes, Chromosomes and Cancer 26(4). p.21-312
Abstract
Chromosome banding analysis of 11 short-term cultured gallbladder carcinomas revealed acquired clonal aberrations in seven tumors (five primary and two metastases). Three of these had one clone, whereas the remaining four were cytogenetically heterogeneous, displaying two to seven aberrant clones. Of a total of 21 abnormal clones, 18 had highly complex karyotypes and three exhibited simple numerical deviations. Double minutes and homogeneously staining regions were observed in one and two carcinomas, respectively. To characterize the karyotypic profile of gallbladder cancer more precisely, we have combined the present findings with our three previously reported cases, thereby providing the largest cytogenetic database on this tumor type to... (More)
Chromosome banding analysis of 11 short-term cultured gallbladder carcinomas revealed acquired clonal aberrations in seven tumors (five primary and two metastases). Three of these had one clone, whereas the remaining four were cytogenetically heterogeneous, displaying two to seven aberrant clones. Of a total of 21 abnormal clones, 18 had highly complex karyotypes and three exhibited simple numerical deviations. Double minutes and homogeneously staining regions were observed in one and two carcinomas, respectively. To characterize the karyotypic profile of gallbladder cancer more precisely, we have combined the present findings with our three previously reported cases, thereby providing the largest cytogenetic database on this tumor type to date. A total of 287 chromosomal breakpoints were identified, 251 of which were found in the present study. Chromosome 7 was rearranged most frequently, followed by chromosomes 1, 3, 11, 6, 5, and 8. The bands preferentially involved were 1p32, 1p36, 1q32, 3p21, 6p21, 7p13, 7q11, 7q32, 19p13, 19q13, and 22q13. Nine recurrent abnormalities could, for the first time, be identified in gallbladder carcinoma: del(3)(p13), i(5)(p10), del(6)(q13), del(9)(p13), del(16)(q22), del(17)(p11), i(17)(q10), del(19)(p13), and i(21)(q10). The most common partial or whole-arm gains involved 3q, 5p, 7p, 7q, 8q, 11q, 13q, and 17q, and the most frequent partial or whole-arm losses affected 3p, 4q, 5q, 9p, 10p, 10q, 11p, 14p, 14q, 15p, 17p, 19p, 21p, 21q, and Xp. These chromosomal aberrations and imbalances provide some starting points for molecular analyses of genomic regions that may harbor genes of pathogenetic importance in gallbladder carcinogenesis. Genes Chromosomes Cancer 26:312-321, 1999. (Less)
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Contribution to journal
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published
subject
keywords
Clone Cells, Middle Aged, Chromosome Aberrations, Aged, Carcinoma/ genetics, Humans, Karyotyping, Male, Genetic Heterogeneity, Female, Gallbladder Neoplasms/ genetics
in
Genes, Chromosomes and Cancer
volume
26
issue
4
pages
21 - 312
publisher
John Wiley & Sons
external identifiers
  • scopus:0032746789
ISSN
1045-2257
language
English
LU publication?
yes
id
6402951a-8e7a-49fa-a7b4-36f60f4b03b6 (old id 3052479)
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http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1098-2264(199912)26:4%3C312::AID-GCC5%3E3.0.CO;2-3/pdf
http://www.ncbi.nlm.nih.gov/pubmed/10534766
date added to LUP
2013-05-07 14:13:59
date last changed
2017-04-09 04:31:56
@article{6402951a-8e7a-49fa-a7b4-36f60f4b03b6,
  abstract     = {Chromosome banding analysis of 11 short-term cultured gallbladder carcinomas revealed acquired clonal aberrations in seven tumors (five primary and two metastases). Three of these had one clone, whereas the remaining four were cytogenetically heterogeneous, displaying two to seven aberrant clones. Of a total of 21 abnormal clones, 18 had highly complex karyotypes and three exhibited simple numerical deviations. Double minutes and homogeneously staining regions were observed in one and two carcinomas, respectively. To characterize the karyotypic profile of gallbladder cancer more precisely, we have combined the present findings with our three previously reported cases, thereby providing the largest cytogenetic database on this tumor type to date. A total of 287 chromosomal breakpoints were identified, 251 of which were found in the present study. Chromosome 7 was rearranged most frequently, followed by chromosomes 1, 3, 11, 6, 5, and 8. The bands preferentially involved were 1p32, 1p36, 1q32, 3p21, 6p21, 7p13, 7q11, 7q32, 19p13, 19q13, and 22q13. Nine recurrent abnormalities could, for the first time, be identified in gallbladder carcinoma: del(3)(p13), i(5)(p10), del(6)(q13), del(9)(p13), del(16)(q22), del(17)(p11), i(17)(q10), del(19)(p13), and i(21)(q10). The most common partial or whole-arm gains involved 3q, 5p, 7p, 7q, 8q, 11q, 13q, and 17q, and the most frequent partial or whole-arm losses affected 3p, 4q, 5q, 9p, 10p, 10q, 11p, 14p, 14q, 15p, 17p, 19p, 21p, 21q, and Xp. These chromosomal aberrations and imbalances provide some starting points for molecular analyses of genomic regions that may harbor genes of pathogenetic importance in gallbladder carcinogenesis. Genes Chromosomes Cancer 26:312-321, 1999.},
  author       = {Gorunova, Ludmila and Parada, Luis Antonio and Limon, J. and Jin, Yuesheng and Hallén, Magnus and Hagerstrand, Inga and Iliszko, M. and Wajda, Z. and Johansson, Bertil},
  issn         = {1045-2257},
  keyword      = {Clone Cells,Middle Aged,Chromosome Aberrations,Aged,Carcinoma/ genetics,Humans,Karyotyping,Male,Genetic Heterogeneity,Female,Gallbladder Neoplasms/ genetics},
  language     = {eng},
  number       = {4},
  pages        = {21--312},
  publisher    = {John Wiley & Sons},
  series       = {Genes, Chromosomes and Cancer},
  title        = {Nonrandom chromosomal aberrations and cytogenetic heterogeneity in gallbladder carcinomas},
  volume       = {26},
  year         = {1999},
}