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The breast cancer-associated gene Di12 has oncogenic activity

Burger, AM; Grugel, R; Landberg, Göran LU ; Kitching, R and Seth, A (2003) In Anticancer research 23(3A). p.2027-2033
Abstract
The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of... (More)
The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
nude mice, mabDi12, tumor formation, Di12, clonogenic assay, gene, expression
in
Anticancer research
volume
23
issue
3A
pages
2027 - 2033
publisher
International Institute of Cancer Research
external identifiers
  • wos:000184359100006
  • scopus:0042306312
ISSN
1791-7530
language
English
LU publication?
yes
id
08d6cf6c-ac6c-46e6-b8d1-3c2c83b61e0f (old id 305794)
date added to LUP
2007-08-27 12:27:12
date last changed
2018-10-03 10:12:19
@article{08d6cf6c-ac6c-46e6-b8d1-3c2c83b61e0f,
  abstract     = {The breast cancer-associated gene Di12 encodes a novel protein, which was found overexpressed in invasive ductal carcinomas of the breast. In experiments designed to assess the role of the Di12 gene in oncogenesis, the overexpression of 339 N-terminal amino acids of this gene in NIH3T3 cells resulted in cellular transformation and in vivo tumorigenesis. NIH3T3-Di12 tumor cell growth was partly reversible upon Di12 antisense treatment. In addition, transfortnation of the ER + human breast cancer cell line MCF- 7 resulted in hormone independent growth of these tumors in nude mice. Di12 expression in NIH3T3 and MCF-7 tumor cells was confirmed by RT-PCR and mabDi12 immunostaining. Immunohistochemistry using mabDi12 on an arrayed collection of 106 invasive breast tumors further underlined the expression of the gene in over 75% of advanced stage breast cancers. Our data indicate that Di12 expression is oncogenic in in vitro transformation and in vivo tumorigenic assays.},
  author       = {Burger, AM and Grugel, R and Landberg, Göran and Kitching, R and Seth, A},
  issn         = {1791-7530},
  keyword      = {nude mice,mabDi12,tumor formation,Di12,clonogenic assay,gene,expression},
  language     = {eng},
  number       = {3A},
  pages        = {2027--2033},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer research},
  title        = {The breast cancer-associated gene Di12 has oncogenic activity},
  volume       = {23},
  year         = {2003},
}