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Gene expression profiling of ductal carcinomas in situ and invasive breast tumors

Seth, A; Kitching, R; Landberg, Göran LU ; Xu, J; Zubovits, J and Burger, AM (2003) In Anticancer research 23(3A). p.2043-2051
Abstract
Comparative and functional genomics are powerful tools to advance the understanding of the molecular basis of cancer. It is believed that genes are epigenetically regulated and, thus, each tumor type and stage will be characterized by a gene expression fingerprint. In this study we identified genes that are differentially expressed in ductal carcinoma in situ and invasive ductal carcinoma of the breast. To isolate genes that are associated with progression of breast cancer we performed differential display and subtractive cloning procedures using matched RNA from normal and tumor tissue. cDNA microarray analysis generated gene expression profiles typical of the transition front in situ to invasive breast cancer when we used mRAA extracted... (More)
Comparative and functional genomics are powerful tools to advance the understanding of the molecular basis of cancer. It is believed that genes are epigenetically regulated and, thus, each tumor type and stage will be characterized by a gene expression fingerprint. In this study we identified genes that are differentially expressed in ductal carcinoma in situ and invasive ductal carcinoma of the breast. To isolate genes that are associated with progression of breast cancer we performed differential display and subtractive cloning procedures using matched RNA from normal and tumor tissue. cDNA microarray analysis generated gene expression profiles typical of the transition front in situ to invasive breast cancer when we used mRAA extracted from a case of low-to intermediate-grade DCIS and a case of high-grade DC1S/IDC. cDNAs from these samples were the probes in a cDNA microarray hybridization to 9183 unique cDAAs representing 8507 genes. Signals from both transcriptomes were obtained for 8083 genes, and the balanced differential expression values between pure DCIS and DCIS/invasive tumors revealed 303 distinct cDNAs with a ratio of > 2. Interferon inducible genes were found to be expressed at the highest level in the pure DCIS sample. Genes most abundantly expressed in the invasive tumor were immunoglobulin heavy constant gamma 3 and calgranulin B. Further analysis of RNA and protein expression in breast tumor cell lines and patient tissue samples revealed that: IGFBP-rP1 is down-regulated in invasive tumors whereas cyclin I protein is regulated by ubiquitination and is associated with ER-negative breast cancers. Conclusion: The known and novel genes discussed here represent targets for molecular characterization during breast cancer development as well as,for designing novel strategies for diagnosis and treatment. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
interferon genes, genomics, breast cancer, gene expression profiling
in
Anticancer research
volume
23
issue
3A
pages
2043 - 2051
publisher
International Institute of Cancer Research
external identifiers
  • wos:000184359100008
  • scopus:0041805434
ISSN
1791-7530
language
English
LU publication?
yes
id
3e046f81-564e-44ef-a7ef-f7e326b05d00 (old id 305800)
date added to LUP
2007-09-21 12:35:20
date last changed
2018-10-14 03:37:27
@article{3e046f81-564e-44ef-a7ef-f7e326b05d00,
  abstract     = {Comparative and functional genomics are powerful tools to advance the understanding of the molecular basis of cancer. It is believed that genes are epigenetically regulated and, thus, each tumor type and stage will be characterized by a gene expression fingerprint. In this study we identified genes that are differentially expressed in ductal carcinoma in situ and invasive ductal carcinoma of the breast. To isolate genes that are associated with progression of breast cancer we performed differential display and subtractive cloning procedures using matched RNA from normal and tumor tissue. cDNA microarray analysis generated gene expression profiles typical of the transition front in situ to invasive breast cancer when we used mRAA extracted from a case of low-to intermediate-grade DCIS and a case of high-grade DC1S/IDC. cDNAs from these samples were the probes in a cDNA microarray hybridization to 9183 unique cDAAs representing 8507 genes. Signals from both transcriptomes were obtained for 8083 genes, and the balanced differential expression values between pure DCIS and DCIS/invasive tumors revealed 303 distinct cDNAs with a ratio of > 2. Interferon inducible genes were found to be expressed at the highest level in the pure DCIS sample. Genes most abundantly expressed in the invasive tumor were immunoglobulin heavy constant gamma 3 and calgranulin B. Further analysis of RNA and protein expression in breast tumor cell lines and patient tissue samples revealed that: IGFBP-rP1 is down-regulated in invasive tumors whereas cyclin I protein is regulated by ubiquitination and is associated with ER-negative breast cancers. Conclusion: The known and novel genes discussed here represent targets for molecular characterization during breast cancer development as well as,for designing novel strategies for diagnosis and treatment.},
  author       = {Seth, A and Kitching, R and Landberg, Göran and Xu, J and Zubovits, J and Burger, AM},
  issn         = {1791-7530},
  keyword      = {interferon genes,genomics,breast cancer,gene expression profiling},
  language     = {eng},
  number       = {3A},
  pages        = {2043--2051},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer research},
  title        = {Gene expression profiling of ductal carcinomas in situ and invasive breast tumors},
  volume       = {23},
  year         = {2003},
}