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Homotopic glial regulation of striatal projection neuron differentiation

Skogh, C and Campbell, Kenneth LU (2003) In NeuroReport 14(7). p.1037-1040
Abstract
We have studied the differentiation of striatal projection neurons in co-culture with expanded glia from different regions of the embryonic mouse telencephalon. Our results show that when striatal progenitors are cultured on glia derived from the same region as they originate (i.e. the lateral ganglionic eminence), the neurons formed exhibit long processes. This is not the case when the cells are co-cultured with glia derived from the adjacent telencephalic region, the medial ganglionic eminence. Moreover, expression of the striatal projection neuron marker, dopamine- and cAMP-regulated phosphoprotein (DARPP-32) was significantly enhanced in neurons cultured on the homotopic glia. Thus, glial cultures derived from the lateral ganglionic... (More)
We have studied the differentiation of striatal projection neurons in co-culture with expanded glia from different regions of the embryonic mouse telencephalon. Our results show that when striatal progenitors are cultured on glia derived from the same region as they originate (i.e. the lateral ganglionic eminence), the neurons formed exhibit long processes. This is not the case when the cells are co-cultured with glia derived from the adjacent telencephalic region, the medial ganglionic eminence. Moreover, expression of the striatal projection neuron marker, dopamine- and cAMP-regulated phosphoprotein (DARPP-32) was significantly enhanced in neurons cultured on the homotopic glia. Thus, glial cultures derived from the lateral ganglionic eminence positively regulate the differentiation of striatal projection neurons in vitro. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
beta-tubulin III, co-culture, DARPP-32
in
NeuroReport
volume
14
issue
7
pages
1037 - 1040
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000184057700026
  • pmid:12802198
  • scopus:0038574307
ISSN
1473-558X
DOI
10.1097/01.wnr.0000073424.02536.22
language
English
LU publication?
yes
id
84c3baf5-2d7f-46a6-99b0-43debe43cc16 (old id 306744)
date added to LUP
2007-09-21 16:35:34
date last changed
2018-05-29 11:08:04
@article{84c3baf5-2d7f-46a6-99b0-43debe43cc16,
  abstract     = {We have studied the differentiation of striatal projection neurons in co-culture with expanded glia from different regions of the embryonic mouse telencephalon. Our results show that when striatal progenitors are cultured on glia derived from the same region as they originate (i.e. the lateral ganglionic eminence), the neurons formed exhibit long processes. This is not the case when the cells are co-cultured with glia derived from the adjacent telencephalic region, the medial ganglionic eminence. Moreover, expression of the striatal projection neuron marker, dopamine- and cAMP-regulated phosphoprotein (DARPP-32) was significantly enhanced in neurons cultured on the homotopic glia. Thus, glial cultures derived from the lateral ganglionic eminence positively regulate the differentiation of striatal projection neurons in vitro.},
  author       = {Skogh, C and Campbell, Kenneth},
  issn         = {1473-558X},
  keyword      = {beta-tubulin III,co-culture,DARPP-32},
  language     = {eng},
  number       = {7},
  pages        = {1037--1040},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {NeuroReport},
  title        = {Homotopic glial regulation of striatal projection neuron differentiation},
  url          = {http://dx.doi.org/10.1097/01.wnr.0000073424.02536.22},
  volume       = {14},
  year         = {2003},
}