Advanced

Similar rates for platination of hairpin loops and single-stranded DNA

Kjellström, Johan LU and Elmroth, Sofi LU (2003) In Dalton Transactions p.2867-2871
Abstract
The presence of ribonucleic acid hairpin structures is important for proper processing of the genetic information in living cells. Recent studies indicate that interactions between platinum based anticancer active metal complexes and hairpin motifs result in a change of their three-dimensional structure combined with a decrease of the melting temperature. We here report a study of the reaction between two platinum complexes, cis-[PtCl(NH3)(2)(OH2)](+) I and, cis-[PtCl(NH3)(c-NH2C6H11)(OH2)](+) II, and a series of short DNA hairpins which all exhibit a documented preference for adduct formation with donor groups located in the loop region; d(CGCGTTXTTCGCG), where X = G-N7, p(S), I-s6 or U-s4. The binding kinetics of adduct formation with... (More)
The presence of ribonucleic acid hairpin structures is important for proper processing of the genetic information in living cells. Recent studies indicate that interactions between platinum based anticancer active metal complexes and hairpin motifs result in a change of their three-dimensional structure combined with a decrease of the melting temperature. We here report a study of the reaction between two platinum complexes, cis-[PtCl(NH3)(2)(OH2)](+) I and, cis-[PtCl(NH3)(c-NH2C6H11)(OH2)](+) II, and a series of short DNA hairpins which all exhibit a documented preference for adduct formation with donor groups located in the loop region; d(CGCGTTXTTCGCG), where X = G-N7, p(S), I-s6 or U-s4. The binding kinetics of adduct formation with the hairpins have been compared with that of binding to similar size single-stranded DNA; d(T6XT6), where X = G, p(S), I-s6 or U-s4. The overall picture reveals rather similar reactivity of the platinum complexes towards these two DNA secondary structures. However, the smaller complex I exhibits a tendency for preferential interaction with the hairpins, whereas the more bulky complex II shows a higher reactivity towards the single-stranded structures. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Dalton Transactions
issue
14
pages
2867 - 2871
publisher
Royal Society of Chemistry
external identifiers
  • wos:000184049700013
  • scopus:1642421141
ISSN
1477-9234
DOI
10.1039/b302477f
language
English
LU publication?
yes
id
05e953a8-89fb-49fe-9726-fb1c7fe227da (old id 306827)
date added to LUP
2007-09-16 09:28:48
date last changed
2018-07-01 03:31:44
@article{05e953a8-89fb-49fe-9726-fb1c7fe227da,
  abstract     = {The presence of ribonucleic acid hairpin structures is important for proper processing of the genetic information in living cells. Recent studies indicate that interactions between platinum based anticancer active metal complexes and hairpin motifs result in a change of their three-dimensional structure combined with a decrease of the melting temperature. We here report a study of the reaction between two platinum complexes, cis-[PtCl(NH3)(2)(OH2)](+) I and, cis-[PtCl(NH3)(c-NH2C6H11)(OH2)](+) II, and a series of short DNA hairpins which all exhibit a documented preference for adduct formation with donor groups located in the loop region; d(CGCGTTXTTCGCG), where X = G-N7, p(S), I-s6 or U-s4. The binding kinetics of adduct formation with the hairpins have been compared with that of binding to similar size single-stranded DNA; d(T6XT6), where X = G, p(S), I-s6 or U-s4. The overall picture reveals rather similar reactivity of the platinum complexes towards these two DNA secondary structures. However, the smaller complex I exhibits a tendency for preferential interaction with the hairpins, whereas the more bulky complex II shows a higher reactivity towards the single-stranded structures.},
  author       = {Kjellström, Johan and Elmroth, Sofi},
  issn         = {1477-9234},
  language     = {eng},
  number       = {14},
  pages        = {2867--2871},
  publisher    = {Royal Society of Chemistry},
  series       = {Dalton Transactions},
  title        = {Similar rates for platination of hairpin loops and single-stranded DNA},
  url          = {http://dx.doi.org/10.1039/b302477f},
  year         = {2003},
}