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Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF

Ask, Magnus; Olofsson, Kim LU ; Di Felice, Tommaso; Ruohonen, Laura; Penttila, Merja; Lidén, Gunnar LU and Olsson, Lisbeth (2012) In Process Biochemistry 47(10). p.1452-1459
Abstract
The perennial herbaceous crop Arundo donax is a potential feedstock for second-generation bioethanol production. In the present work, two different process options were investigated for the conversion of two differently steam-pretreated batches of A. donax. The pretreated raw material was converted to ethanol with a xylose-consuming Saccharomyces cerevisiae strain, VTT C-10880, by applying either separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF). The highest overall ethanol yield and final ethanol concentration were achieved using SHF (0.27 g g(-1) and 20.6 g L-1 compared to 0.24 g g(-1) and 19.0 g L-1 when SSF was used). The performance of both SHF and SSF was improved by complementing the... (More)
The perennial herbaceous crop Arundo donax is a potential feedstock for second-generation bioethanol production. In the present work, two different process options were investigated for the conversion of two differently steam-pretreated batches of A. donax. The pretreated raw material was converted to ethanol with a xylose-consuming Saccharomyces cerevisiae strain, VTT C-10880, by applying either separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF). The highest overall ethanol yield and final ethanol concentration were achieved using SHF (0.27 g g(-1) and 20.6 g L-1 compared to 0.24 g g(-1) and 19.0 g L-1 when SSF was used). The performance of both SHF and SSF was improved by complementing the cellulolytic enzymes with hemicellulases. The higher amount of acetic acid in one of the batches was shown to strongly affect xylose consumption in the fermentation. Only half of the xylose was consumed when batch 1 (high acetic acid) was fermented, compared to that 94% of the xylose was consumed in fermentation of batch 2 (lower acetic acid). Furthermore, the high amount of xylooligomers present in the pretreated materials considerably inhibited the enzymatic hydrolysis. Both the formation of xylooligomers and acetic acid thus need to be considered in the pretreatment process in order to achieve efficient conversion of A. donax to ethanol. (c) 2012 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Arundo donax, Giant reed, SHF, SSF, Acetic acid stress, Xylooligomers
in
Process Biochemistry
volume
47
issue
10
pages
1452 - 1459
publisher
Elsevier
external identifiers
  • wos:000307203100002
  • scopus:84863793778
ISSN
1873-3298
DOI
10.1016/j.procbio.2012.05.016
language
English
LU publication?
yes
id
dfb32857-581f-4523-940c-9e12a3fe79a0 (old id 3069399)
date added to LUP
2012-09-27 13:00:15
date last changed
2017-05-28 03:09:57
@article{dfb32857-581f-4523-940c-9e12a3fe79a0,
  abstract     = {The perennial herbaceous crop Arundo donax is a potential feedstock for second-generation bioethanol production. In the present work, two different process options were investigated for the conversion of two differently steam-pretreated batches of A. donax. The pretreated raw material was converted to ethanol with a xylose-consuming Saccharomyces cerevisiae strain, VTT C-10880, by applying either separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF). The highest overall ethanol yield and final ethanol concentration were achieved using SHF (0.27 g g(-1) and 20.6 g L-1 compared to 0.24 g g(-1) and 19.0 g L-1 when SSF was used). The performance of both SHF and SSF was improved by complementing the cellulolytic enzymes with hemicellulases. The higher amount of acetic acid in one of the batches was shown to strongly affect xylose consumption in the fermentation. Only half of the xylose was consumed when batch 1 (high acetic acid) was fermented, compared to that 94% of the xylose was consumed in fermentation of batch 2 (lower acetic acid). Furthermore, the high amount of xylooligomers present in the pretreated materials considerably inhibited the enzymatic hydrolysis. Both the formation of xylooligomers and acetic acid thus need to be considered in the pretreatment process in order to achieve efficient conversion of A. donax to ethanol. (c) 2012 Elsevier Ltd. All rights reserved.},
  author       = {Ask, Magnus and Olofsson, Kim and Di Felice, Tommaso and Ruohonen, Laura and Penttila, Merja and Lidén, Gunnar and Olsson, Lisbeth},
  issn         = {1873-3298},
  keyword      = {Arundo donax,Giant reed,SHF,SSF,Acetic acid stress,Xylooligomers},
  language     = {eng},
  number       = {10},
  pages        = {1452--1459},
  publisher    = {Elsevier},
  series       = {Process Biochemistry},
  title        = {Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF},
  url          = {http://dx.doi.org/10.1016/j.procbio.2012.05.016},
  volume       = {47},
  year         = {2012},
}