Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities
(2004) In Development: For advances in developmental biology and stem cells 131(8). p.1847-1857- Abstract
- Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development, but the relative importance of different cellular sources of PDGFB has not been established. Using Cre-lox techniques, we show here that genetic ablation of Pdgfb in endothelial cells leads to impaired recruitment of pericytes to blood vessels. The endothelium-restricted Pdgfb knockout mutants also developed organ defects including cardiac, placental and renal abnormalities. These defects were similar to those observed in Pdgfb null mice. However, in marked contrast to the embryonic lethality of Pdgfb null mutants, the endothelium-specific mutants survived into adulthood with persistent pathological changes, including brain microhemorrhages, focal... (More)
- Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development, but the relative importance of different cellular sources of PDGFB has not been established. Using Cre-lox techniques, we show here that genetic ablation of Pdgfb in endothelial cells leads to impaired recruitment of pericytes to blood vessels. The endothelium-restricted Pdgfb knockout mutants also developed organ defects including cardiac, placental and renal abnormalities. These defects were similar to those observed in Pdgfb null mice. However, in marked contrast to the embryonic lethality of Pdgfb null mutants, the endothelium-specific mutants survived into adulthood with persistent pathological changes, including brain microhemorrhages, focal astrogliosis, and kidney glomerulus abnormalities. This spectrum of pathological changes is reminiscent of diabetic microangiopathy, suggesting that the endothelium-restricted Pdgfb knockouts may serve as models for some of the pathogenic events of vascular complications to diabetes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/279925
- author
- Bjarnegard, M ; Enge, M ; Norlin, J ; Gustafsdottir, S ; Fredriksson, S ; Abramsson, A ; Takemoto, M ; Gustafsson, Erika LU ; Fassler, R and Betsholtz, C
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- PDGFB, Cre, loxP, microaneurysm, pericytes, endothelium
- in
- Development: For advances in developmental biology and stem cells
- volume
- 131
- issue
- 8
- pages
- 1847 - 1857
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- pmid:15084468
- wos:000221155900016
- scopus:2342431290
- ISSN
- 1477-9129
- DOI
- 10.1242/10.1242/dev.01080
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
- id
- 306946ab-ca7e-4acf-86a1-746cc66f3a86 (old id 279925)
- date added to LUP
- 2016-04-01 12:15:27
- date last changed
- 2022-04-21 04:53:18
@article{306946ab-ca7e-4acf-86a1-746cc66f3a86,
abstract = {{Platelet-derived growth factor-B (PDGFB) is necessary for normal cardiovascular development, but the relative importance of different cellular sources of PDGFB has not been established. Using Cre-lox techniques, we show here that genetic ablation of Pdgfb in endothelial cells leads to impaired recruitment of pericytes to blood vessels. The endothelium-restricted Pdgfb knockout mutants also developed organ defects including cardiac, placental and renal abnormalities. These defects were similar to those observed in Pdgfb null mice. However, in marked contrast to the embryonic lethality of Pdgfb null mutants, the endothelium-specific mutants survived into adulthood with persistent pathological changes, including brain microhemorrhages, focal astrogliosis, and kidney glomerulus abnormalities. This spectrum of pathological changes is reminiscent of diabetic microangiopathy, suggesting that the endothelium-restricted Pdgfb knockouts may serve as models for some of the pathogenic events of vascular complications to diabetes.}},
author = {{Bjarnegard, M and Enge, M and Norlin, J and Gustafsdottir, S and Fredriksson, S and Abramsson, A and Takemoto, M and Gustafsson, Erika and Fassler, R and Betsholtz, C}},
issn = {{1477-9129}},
keywords = {{PDGFB; Cre; loxP; microaneurysm; pericytes; endothelium}},
language = {{eng}},
number = {{8}},
pages = {{1847--1857}},
publisher = {{The Company of Biologists Ltd}},
series = {{Development: For advances in developmental biology and stem cells}},
title = {{Endothelium-specific ablation of PDGFB leads to pericyte loss and glomerular, cardiac and placental abnormalities}},
url = {{http://dx.doi.org/10.1242/10.1242/dev.01080}},
doi = {{10.1242/10.1242/dev.01080}},
volume = {{131}},
year = {{2004}},
}