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Rapid and convenient determination of oxalic acid employing a novel oxalate biosensor based on oxalate oxidase and SIRE technology

Hong, Feng LU ; Nilvebrant, N O and Jonsson, L J (2003) In Biosensors & Bioelectronics 18(9). p.1173-1181
Abstract
A new method for rapid determination of oxalic acid was developed using oxalate oxidase and a biosensor based on SIRE (sensors based on injection of the recognition element) technology. The method was selective, simple, fast, and cheap compared with other present detection systems for oxalate. The total analysis time for each assay was 2-9 min. A linear range was observed between 0 and 5 mM when the reaction conditions were 30 degreesC and 60 s. The linear range and upper limit for concentration determination could be increased to 25 mM by shortening the reaction time. The lower limit of detection in standard solutions, 20 muM, could be achieved by means of modification of the reaction conditions, namely increasing the temperature and the... (More)
A new method for rapid determination of oxalic acid was developed using oxalate oxidase and a biosensor based on SIRE (sensors based on injection of the recognition element) technology. The method was selective, simple, fast, and cheap compared with other present detection systems for oxalate. The total analysis time for each assay was 2-9 min. A linear range was observed between 0 and 5 mM when the reaction conditions were 30 degreesC and 60 s. The linear range and upper limit for concentration determination could be increased to 25 mM by shortening the reaction time. The lower limit of detection in standard solutions, 20 muM, could be achieved by means of modification of the reaction conditions, namely increasing the temperature and the reaction time. The biosensor method was compared with a conventional commercially available colorimetric method with respect to the determination of oxalic acid in urine samples. The urine oxalic acid concentrations determined with the biosensor method correlated well (R = 0.952) with the colorimetric method. (C) 2002 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
SIRE technology, biosensor, oxalic acid, oxalate oxidase
in
Biosensors & Bioelectronics
volume
18
issue
9
pages
1173 - 1181
publisher
Elsevier
external identifiers
  • wos:000183942800011
  • pmid:12788560
  • scopus:0038699080
ISSN
1873-4235
DOI
10.1016/S0956-5663(02)00250-6
language
English
LU publication?
yes
id
efded8c8-f0ff-4af1-ac47-69f01fcd2dc6 (old id 307882)
date added to LUP
2007-09-13 11:05:09
date last changed
2018-07-15 04:17:10
@article{efded8c8-f0ff-4af1-ac47-69f01fcd2dc6,
  abstract     = {A new method for rapid determination of oxalic acid was developed using oxalate oxidase and a biosensor based on SIRE (sensors based on injection of the recognition element) technology. The method was selective, simple, fast, and cheap compared with other present detection systems for oxalate. The total analysis time for each assay was 2-9 min. A linear range was observed between 0 and 5 mM when the reaction conditions were 30 degreesC and 60 s. The linear range and upper limit for concentration determination could be increased to 25 mM by shortening the reaction time. The lower limit of detection in standard solutions, 20 muM, could be achieved by means of modification of the reaction conditions, namely increasing the temperature and the reaction time. The biosensor method was compared with a conventional commercially available colorimetric method with respect to the determination of oxalic acid in urine samples. The urine oxalic acid concentrations determined with the biosensor method correlated well (R = 0.952) with the colorimetric method. (C) 2002 Elsevier Science B.V. All rights reserved.},
  author       = {Hong, Feng and Nilvebrant, N O and Jonsson, L J},
  issn         = {1873-4235},
  keyword      = {SIRE technology,biosensor,oxalic acid,oxalate oxidase},
  language     = {eng},
  number       = {9},
  pages        = {1173--1181},
  publisher    = {Elsevier},
  series       = {Biosensors & Bioelectronics},
  title        = {Rapid and convenient determination of oxalic acid employing a novel oxalate biosensor based on oxalate oxidase and SIRE technology},
  url          = {http://dx.doi.org/10.1016/S0956-5663(02)00250-6},
  volume       = {18},
  year         = {2003},
}