Advanced

Capturing ion exchanger-bound infectious pancreatic necrosis virus - design and application for large volume water samples

Eliasson, Linda LU ; Sydoff, Stefan LU and Everitt, Einar LU (2003) In Journal of Virological Methods 110(2). p.173-178
Abstract
A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 1 water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide eel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total... (More)
A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 1 water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide eel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ion exchanger, IPNV, virus concentration, filtration device
in
Journal of Virological Methods
volume
110
issue
2
pages
173 - 178
publisher
Elsevier
external identifiers
  • wos:000183669500008
  • pmid:12798245
  • scopus:0038392715
ISSN
1879-0984
DOI
10.1016/S0166-0934(03)00126-5
language
English
LU publication?
yes
id
b5ce15cc-3be9-44a8-a8e8-43d5547437c4 (old id 308468)
date added to LUP
2007-08-29 15:01:14
date last changed
2018-01-07 06:25:52
@article{b5ce15cc-3be9-44a8-a8e8-43d5547437c4,
  abstract     = {A method was designed for rapid and reliable demonstration of the presence of infectious pancreatic necrosis virus retrieved from 5 1 water samples. Viruses together with an added carrier protein were adsorbed to a resin of an added anion exchanger. Then the resin was collected rapidly and quantitatively through a specific device which we designed. The resin was transferred to a column from which the viruses were eluted, and subsequently further concentrated by acid precipitation, followed by SDS-polyacrylamide eel electrophoresis and Western blot analysis. Serological results were obtained within 24 h after the water sample was introduced into the laboratory. Proteins were recovered at an efficiency between 70 and 80% and the total concentration factor ranged between 150000 and 250000 times, depending on the requirements and the methods of choice.},
  author       = {Eliasson, Linda and Sydoff, Stefan and Everitt, Einar},
  issn         = {1879-0984},
  keyword      = {ion exchanger,IPNV,virus concentration,filtration device},
  language     = {eng},
  number       = {2},
  pages        = {173--178},
  publisher    = {Elsevier},
  series       = {Journal of Virological Methods},
  title        = {Capturing ion exchanger-bound infectious pancreatic necrosis virus - design and application for large volume water samples},
  url          = {http://dx.doi.org/10.1016/S0166-0934(03)00126-5},
  volume       = {110},
  year         = {2003},
}