Advanced

Partial outlet obstruction enhances modular autonomous activity in the isolated rat bladder

Drake, MJ; Hedlund, Petter LU ; Harvey, IJ; Pandita, Raj LU ; Andersson, Karl-Erik LU and Gillespie, JI (2003) In Journal of Urology 170(1). p.276-279
Abstract
Purpose: Autonomous bladder activity can take the form of localized micromotions (MMs), suggesting that the detrusor may be arranged into component modules, of which each is capable of contracting autonomously. We examined MMs in isolated whole rat bladder and the effects of partial bladder outlet obstruction as a model of detrusor overactivity (DO) to ascertain whether altered modular activity could be an etiological factor in DO. Materials and Methods: A total of 12 adult female Sprague-Dawley rats underwent obstruction or sham operation for 1 or 4 weeks. Bladders were microsurgically removed and mounted in whole organ tissue baths. Recordings of intravesical pressure and simultaneous registration of intramural contractions were... (More)
Purpose: Autonomous bladder activity can take the form of localized micromotions (MMs), suggesting that the detrusor may be arranged into component modules, of which each is capable of contracting autonomously. We examined MMs in isolated whole rat bladder and the effects of partial bladder outlet obstruction as a model of detrusor overactivity (DO) to ascertain whether altered modular activity could be an etiological factor in DO. Materials and Methods: A total of 12 adult female Sprague-Dawley rats underwent obstruction or sham operation for 1 or 4 weeks. Bladders were microsurgically removed and mounted in whole organ tissue baths. Recordings of intravesical pressure and simultaneous registration of intramural contractions were performed under standardized conditions. Results: Prior to filling MMs took the form of localized contractions near the vesicoureteral junction in sham operated animals and multifocal microcontractions in obstructed animals. Intravesical volume increases were associated with a change in localized MMs to propagated contraction waves. In sham operated animals stretch resulted in increased MM frequency but decreased amplitude. After obstruction stretch elicited highly coordinated MMs and enhanced intravesical pressure transmission. The time since surgery did not alter observations in the sham or obstructed group. Conclusions: Detrusor muscle in isolated bladders under conditions modeling urine storage may have a functional modular arrangement with the basolateral region most active prior to filling. Peripheral factors determining intravesical pressure include the number of modules active, coordination and intramural tension at other sites. After bladder outlet obstruction more modules are active at baseline and their coordination is enhanced by stretch, leading to increased pressure fluctuations. Such changes may contribute to the development of DO. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
urinary incontinence, bladder, smooth, muscle, rats, Sprague-Dawley, bladder neck obstruction
in
Journal of Urology
volume
170
issue
1
pages
276 - 279
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000183593800079
  • pmid:12796704
  • scopus:0038679276
ISSN
1527-3792
DOI
language
English
LU publication?
yes
id
a879695a-3cbb-41e9-9791-58a2daadfea2 (old id 308475)
date added to LUP
2007-08-29 12:37:58
date last changed
2018-05-29 10:17:45
@article{a879695a-3cbb-41e9-9791-58a2daadfea2,
  abstract     = {Purpose: Autonomous bladder activity can take the form of localized micromotions (MMs), suggesting that the detrusor may be arranged into component modules, of which each is capable of contracting autonomously. We examined MMs in isolated whole rat bladder and the effects of partial bladder outlet obstruction as a model of detrusor overactivity (DO) to ascertain whether altered modular activity could be an etiological factor in DO. Materials and Methods: A total of 12 adult female Sprague-Dawley rats underwent obstruction or sham operation for 1 or 4 weeks. Bladders were microsurgically removed and mounted in whole organ tissue baths. Recordings of intravesical pressure and simultaneous registration of intramural contractions were performed under standardized conditions. Results: Prior to filling MMs took the form of localized contractions near the vesicoureteral junction in sham operated animals and multifocal microcontractions in obstructed animals. Intravesical volume increases were associated with a change in localized MMs to propagated contraction waves. In sham operated animals stretch resulted in increased MM frequency but decreased amplitude. After obstruction stretch elicited highly coordinated MMs and enhanced intravesical pressure transmission. The time since surgery did not alter observations in the sham or obstructed group. Conclusions: Detrusor muscle in isolated bladders under conditions modeling urine storage may have a functional modular arrangement with the basolateral region most active prior to filling. Peripheral factors determining intravesical pressure include the number of modules active, coordination and intramural tension at other sites. After bladder outlet obstruction more modules are active at baseline and their coordination is enhanced by stretch, leading to increased pressure fluctuations. Such changes may contribute to the development of DO.},
  author       = {Drake, MJ and Hedlund, Petter and Harvey, IJ and Pandita, Raj and Andersson, Karl-Erik and Gillespie, JI},
  issn         = {1527-3792},
  keyword      = {urinary incontinence,bladder,smooth,muscle,rats,Sprague-Dawley,bladder neck obstruction},
  language     = {eng},
  number       = {1},
  pages        = {276--279},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Partial outlet obstruction enhances modular autonomous activity in the isolated rat bladder},
  url          = {http://dx.doi.org/},
  volume       = {170},
  year         = {2003},
}