Unique antitumour effects of L-2,4 diaminobutyric acid on cultured hepatoma cells
(2003) In Anticancer research 23(2B). p.1245-1248- Abstract
- A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric... (More)
- A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric acid and N-methyl a-aminoisobutyric acid in the incubation medium did not influence the viability of hepatoma cells. We conclude that the tumour cell destructive effect of DAB was the result of a huge and unlimited uptake of DAB energized by the Na+-gradient and that this uptake was not subjected to the law of saturation kinetics. This was combined with a tumour cell energy crisis in attempts to restore the Na+-gradient. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/308706
- author
- Blind, Per-Jonas LU ; Waldenström Ellervik, Alexandra LU ; Hafstrom, L ; Berggren, D and Ronquist, G
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- alpha-aminoisobutyric acid, antitumour activity, diaminobutyric acid, hepatoma cells, N-methyl-alpha-aminoisobutyric acid
- in
- Anticancer research
- volume
- 23
- issue
- 2B
- pages
- 1245 - 1248
- publisher
- International Institute of Cancer Research
- external identifiers
-
- wos:000183471600012
- scopus:0038238328
- ISSN
- 1791-7530
- language
- English
- LU publication?
- yes
- id
- f35d4353-a909-4110-bfaf-17ab12b7768c (old id 308706)
- date added to LUP
- 2016-04-01 12:38:30
- date last changed
- 2022-01-27 07:50:30
@article{f35d4353-a909-4110-bfaf-17ab12b7768c,
abstract = {{A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric acid and N-methyl a-aminoisobutyric acid in the incubation medium did not influence the viability of hepatoma cells. We conclude that the tumour cell destructive effect of DAB was the result of a huge and unlimited uptake of DAB energized by the Na+-gradient and that this uptake was not subjected to the law of saturation kinetics. This was combined with a tumour cell energy crisis in attempts to restore the Na+-gradient.}},
author = {{Blind, Per-Jonas and Waldenström Ellervik, Alexandra and Hafstrom, L and Berggren, D and Ronquist, G}},
issn = {{1791-7530}},
keywords = {{alpha-aminoisobutyric acid; antitumour activity; diaminobutyric acid; hepatoma cells; N-methyl-alpha-aminoisobutyric acid}},
language = {{eng}},
number = {{2B}},
pages = {{1245--1248}},
publisher = {{International Institute of Cancer Research}},
series = {{Anticancer research}},
title = {{Unique antitumour effects of L-2,4 diaminobutyric acid on cultured hepatoma cells}},
volume = {{23}},
year = {{2003}},
}