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Unique antitumour effects of L-2,4 diaminobutyric acid on cultured hepatoma cells

Blind, Per-Jonas LU ; Waldenström Ellervik, Alexandra LU ; Hafstrom, L; Berggren, D and Ronquist, G (2003) In Anticancer Research 23(2B). p.1245-1248
Abstract
A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric... (More)
A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric acid and N-methyl a-aminoisobutyric acid in the incubation medium did not influence the viability of hepatoma cells. We conclude that the tumour cell destructive effect of DAB was the result of a huge and unlimited uptake of DAB energized by the Na+-gradient and that this uptake was not subjected to the law of saturation kinetics. This was combined with a tumour cell energy crisis in attempts to restore the Na+-gradient. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alpha-aminoisobutyric acid, antitumour activity, diaminobutyric acid, hepatoma cells, N-methyl-alpha-aminoisobutyric acid
in
Anticancer Research
volume
23
issue
2B
pages
1245 - 1248
publisher
International Institute of Cancer Research
external identifiers
  • wos:000183471600012
  • scopus:0038238328
ISSN
1791-7530
language
English
LU publication?
yes
id
f35d4353-a909-4110-bfaf-17ab12b7768c (old id 308706)
date added to LUP
2007-08-24 12:02:08
date last changed
2018-01-07 06:27:21
@article{f35d4353-a909-4110-bfaf-17ab12b7768c,
  abstract     = {A single hepatoma cell line was grown in vitro and incubated with L-2,4 diaminobutyric acid (DAB), a non-metabolizable amino acid, under various conditions. The tumour cells were irreversibly damaged by incubation for 8 hours with 8 mmol/L of DAB. The tumour cell-destroying effect of DAB was dose- and time-dependent with no effect at a DAB concentration of 1.6 mmol/L. The presence of N-methyl a-aminoisobutyric acid (a specific substrate of amino acid transport system A) in the incubation medium abrogated the tumour cell destructive effect of DAB in a dose- dependent fashion. The presence of it on-physiological amino acids in the incubation medium per se was not the cause of tumour cell destruction, since inclusion of a-amino-isobutyric acid and N-methyl a-aminoisobutyric acid in the incubation medium did not influence the viability of hepatoma cells. We conclude that the tumour cell destructive effect of DAB was the result of a huge and unlimited uptake of DAB energized by the Na+-gradient and that this uptake was not subjected to the law of saturation kinetics. This was combined with a tumour cell energy crisis in attempts to restore the Na+-gradient.},
  author       = {Blind, Per-Jonas and Waldenström Ellervik, Alexandra and Hafstrom, L and Berggren, D and Ronquist, G},
  issn         = {1791-7530},
  keyword      = {alpha-aminoisobutyric acid,antitumour activity,diaminobutyric acid,hepatoma cells,N-methyl-alpha-aminoisobutyric acid},
  language     = {eng},
  number       = {2B},
  pages        = {1245--1248},
  publisher    = {International Institute of Cancer Research},
  series       = {Anticancer Research},
  title        = {Unique antitumour effects of L-2,4 diaminobutyric acid on cultured hepatoma cells},
  volume       = {23},
  year         = {2003},
}