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Rat stomach ECL cells: mode of activation of histidine decarboxylase

Zhao, CM; Chen, D; Yamada, H; de la Cour, Charlotta LU ; Lindstrom, E; Persson, L and Håkanson, Rolf LU (2003) In Regulatory Peptides 114(1). p.21-27
Abstract
Histidine decarboxylase (HDC) occurs in ECL cells in the oxyntic mucosa of rat stomach. It is activated by gastrin. Refeeding of fasted rats or treatment with the proton pump inhibitor omeprazole promptly raised the serum gastrin concentration and consequently the HDC activity and the HDC protein content of the oxyntic mucosa. The food- and omeprazole-induced increase in HDC mRNA expression in the oxyntic mucosa was modest by comparison. Blockade of translation (cycloheximide) but not transcription (actinomycin D) prevented the postprandial rise in HDC activity. The half-life of HDC activity (after blockade of translation) was 94 min in omeprazole-treated rats and 55 min in fasted controls. The rate of enzyme synthesis was estimated to be... (More)
Histidine decarboxylase (HDC) occurs in ECL cells in the oxyntic mucosa of rat stomach. It is activated by gastrin. Refeeding of fasted rats or treatment with the proton pump inhibitor omeprazole promptly raised the serum gastrin concentration and consequently the HDC activity and the HDC protein content of the oxyntic mucosa. The food- and omeprazole-induced increase in HDC mRNA expression in the oxyntic mucosa was modest by comparison. Blockade of translation (cycloheximide) but not transcription (actinomycin D) prevented the postprandial rise in HDC activity. The half-life of HDC activity (after blockade of translation) was 94 min in omeprazole-treated rats and 55 min in fasted controls. The rate of enzyme synthesis was estimated to be 15 times higher in omeprazole-treated rats than in fasted controls. Inhibition of histamine uptake into ECL-cell granules by reserpine, a blocker of the vesicular monoamine transporter type-2, lowered the HDC activity and prevented the gastrin-induced HDC activation. We suggest that HDC activation reflects enhanced transcription, translation and/or posttranslational enzyme activation as well as stabilization, and that a high cytosolic histamine concentration suppresses HDC activation. (C) 2003 Elsevier Science B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
translation/posttranslation, transcription, rat stomach, protein, HDC, histidine decarboxylase (HDC) activity, ECL cells, histamine
in
Regulatory Peptides
volume
114
issue
1
pages
21 - 27
publisher
Elsevier
external identifiers
  • wos:000183437400004
  • pmid:12763636
  • scopus:0038065360
ISSN
1873-1686
DOI
10.1016/S0167-0115(03)00063-6
language
English
LU publication?
yes
id
30e6a886-5aea-4107-86b4-cbe6f7a8b188 (old id 308806)
date added to LUP
2007-09-24 15:53:57
date last changed
2018-01-07 05:40:14
@article{30e6a886-5aea-4107-86b4-cbe6f7a8b188,
  abstract     = {Histidine decarboxylase (HDC) occurs in ECL cells in the oxyntic mucosa of rat stomach. It is activated by gastrin. Refeeding of fasted rats or treatment with the proton pump inhibitor omeprazole promptly raised the serum gastrin concentration and consequently the HDC activity and the HDC protein content of the oxyntic mucosa. The food- and omeprazole-induced increase in HDC mRNA expression in the oxyntic mucosa was modest by comparison. Blockade of translation (cycloheximide) but not transcription (actinomycin D) prevented the postprandial rise in HDC activity. The half-life of HDC activity (after blockade of translation) was 94 min in omeprazole-treated rats and 55 min in fasted controls. The rate of enzyme synthesis was estimated to be 15 times higher in omeprazole-treated rats than in fasted controls. Inhibition of histamine uptake into ECL-cell granules by reserpine, a blocker of the vesicular monoamine transporter type-2, lowered the HDC activity and prevented the gastrin-induced HDC activation. We suggest that HDC activation reflects enhanced transcription, translation and/or posttranslational enzyme activation as well as stabilization, and that a high cytosolic histamine concentration suppresses HDC activation. (C) 2003 Elsevier Science B.V. All rights reserved.},
  author       = {Zhao, CM and Chen, D and Yamada, H and de la Cour, Charlotta and Lindstrom, E and Persson, L and Håkanson, Rolf},
  issn         = {1873-1686},
  keyword      = {translation/posttranslation,transcription,rat stomach,protein,HDC,histidine decarboxylase (HDC) activity,ECL cells,histamine},
  language     = {eng},
  number       = {1},
  pages        = {21--27},
  publisher    = {Elsevier},
  series       = {Regulatory Peptides},
  title        = {Rat stomach ECL cells: mode of activation of histidine decarboxylase},
  url          = {http://dx.doi.org/10.1016/S0167-0115(03)00063-6},
  volume       = {114},
  year         = {2003},
}