NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea
(2003) In European Journal of Biochemistry 270(11). p.2505-2512- Abstract
- The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/310636
- author
- Tossavainen, H ; Permi, P ; Annila, A ; Kilpelainen, I and Drakenberg, Torbjörn LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- calcium-binding, calerythrin, NMR, structure
- in
- European Journal of Biochemistry
- volume
- 270
- issue
- 11
- pages
- 2505 - 2512
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000183030700019
- pmid:12755706
- scopus:0038392574
- ISSN
- 0014-2956
- DOI
- 10.1046/j.1432-1033.2003.03623.x
- language
- English
- LU publication?
- yes
- id
- f15b421c-6065-4c4f-841f-2c392904a833 (old id 310636)
- alternative location
- http://www.ejbiochem.org/cgi/content/abstract/270/11/2505
- date added to LUP
- 2016-04-01 12:09:21
- date last changed
- 2022-01-26 23:36:19
@article{f15b421c-6065-4c4f-841f-2c392904a833, abstract = {{The structure of calerythrin, a prokaryotic 20 kDa calcium-binding protein has been determined by solution NMR spectroscopy. Distance, dihedral angle, J coupling, secondary chemical shift, residual dipolar coupling and radius of gyration restraints reveal four EF-hand motifs arranged in a compact globular structure. A tight turn in the middle of the amino acid sequence brings the two halves, each comprising a pair of EF-hands, close together. The structural similarity between calerythrin and the eukaryotic sarcoplasmic calcium-binding proteins is notable.}}, author = {{Tossavainen, H and Permi, P and Annila, A and Kilpelainen, I and Drakenberg, Torbjörn}}, issn = {{0014-2956}}, keywords = {{calcium-binding; calerythrin; NMR; structure}}, language = {{eng}}, number = {{11}}, pages = {{2505--2512}}, publisher = {{Wiley-Blackwell}}, series = {{European Journal of Biochemistry}}, title = {{NMR solution structure of calerythrin, an EF-hand calcium-binding protein from Saccharopolyspora erythraea}}, url = {{http://dx.doi.org/10.1046/j.1432-1033.2003.03623.x}}, doi = {{10.1046/j.1432-1033.2003.03623.x}}, volume = {{270}}, year = {{2003}}, }