Direct electrochemical oxidation of DNA on polycrystalline gold electrodes
(2003) In Electroanalysis 15(7). p.629-634- Abstract
- Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance... (More)
- Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/310645
- author
- Ferapontova, Elena LU and Dominguez, E
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- electrochemical oxidation of DNA, electrochemical impedance, gold, guanine
- in
- Electroanalysis
- volume
- 15
- issue
- 7
- pages
- 629 - 634
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000182962500005
- scopus:0038690412
- ISSN
- 1040-0397
- DOI
- 10.1002/elan.200390079
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 0e163984-cfd4-4209-815a-faed0a2cfd7c (old id 310645)
- date added to LUP
- 2016-04-01 16:25:19
- date last changed
- 2022-01-28 19:34:07
@article{0e163984-cfd4-4209-815a-faed0a2cfd7c, abstract = {{Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA.}}, author = {{Ferapontova, Elena and Dominguez, E}}, issn = {{1040-0397}}, keywords = {{electrochemical oxidation of DNA; electrochemical impedance; gold; guanine}}, language = {{eng}}, number = {{7}}, pages = {{629--634}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Electroanalysis}}, title = {{Direct electrochemical oxidation of DNA on polycrystalline gold electrodes}}, url = {{http://dx.doi.org/10.1002/elan.200390079}}, doi = {{10.1002/elan.200390079}}, volume = {{15}}, year = {{2003}}, }