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Direct electrochemical oxidation of DNA on polycrystalline gold electrodes

Ferapontova, Elena LU and Dominguez, E (2003) In Electroanalysis 15(7). p.629-634
Abstract
Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance... (More)
Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
electrochemical oxidation of DNA, electrochemical impedance, gold, guanine
in
Electroanalysis
volume
15
issue
7
pages
629 - 634
publisher
John Wiley & Sons
external identifiers
  • wos:000182962500005
  • scopus:0038690412
ISSN
1040-0397
DOI
language
English
LU publication?
yes
id
0e163984-cfd4-4209-815a-faed0a2cfd7c (old id 310645)
date added to LUP
2007-09-03 07:54:31
date last changed
2018-05-29 10:37:15
@article{0e163984-cfd4-4209-815a-faed0a2cfd7c,
  abstract     = {Electrochemical CV and SWV studies were performed with double stranded DNA from salmon testes (dsDNA) and single stranded DNAs, containing 25 nucleotides (ssDNA) directly adsorbed at polycrystalline An electrodes. A distinct oxidation peak at + 730 mV (SWV, scan rate 0.248 V s(-1)) or at + 730 - + 780 mV (CV, scan rate from 0.3 to 1 V s(-1)) was obtained with DNA-modified Au electrodes after a time-dependent prepolarization step at a positive potential value, i.e., at + 500 mV (vs. Ag AgCl), performed with the DNA-modified Au electrodes dipped in a blank buffer solution. No electrochemical activity was detected when ssDNA, containing no guanines, was used for adsorptive modification of the Au electrodes. Electrochemical impedance measurements registered a possible reorganization of the adsorbed DNA layer in the course of the prepolarization, accompanied by decreasing in-phase impedance. ne results enable us to relate the oxidation process observed at the DNA-modified Au electrodes with the oxidation of guanine residues in DNA.},
  author       = {Ferapontova, Elena and Dominguez, E},
  issn         = {1040-0397},
  keyword      = {electrochemical oxidation of DNA,electrochemical impedance,gold,guanine},
  language     = {eng},
  number       = {7},
  pages        = {629--634},
  publisher    = {John Wiley & Sons},
  series       = {Electroanalysis},
  title        = {Direct electrochemical oxidation of DNA on polycrystalline gold electrodes},
  url          = {http://dx.doi.org/},
  volume       = {15},
  year         = {2003},
}