Advanced

Helicobacter pylori lipopolysaccharide in the IL-2 milieu activates lymphocytes from dyspeptic children

Rudnicka, W; Jarosinska, A; Bak-Romaniszyn, L; Moran, A; Planeta-Malecka, I; Wadström, Torkel LU and Chmiela, M (2003) 5th International Workshop on Pathogenesis and Host Response in Helicobacter Infections In FEMS Immunology and Medical Microbiology (Helicobacter Pathogenesis and Immunology. Selected papers) 36(3). p.141-145
Abstract
In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the... (More)
In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the presence of IL-2, to IL-2 alone and to H. pylori LPS+IL-2. However, there was no difference in PHA-driven proliferation of PBML from the patients of those two groups. A negative correlation between the responsiveness to H. pylori LPS of PBML and occurrence of type B inflammation in gastric mucosa was demonstrated. The results suggest a contribution of H. pylori LPS to an outcome of H. pylori infection. It is speculated that H. pylori LPS by an activation of immunocompetent cells may reduce gastric inflammation, decrease bacterial load and prolong H. pylori infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
LPS, IL-2, Helicobacter pylori, inflammation, cell proliferation
in
FEMS Immunology and Medical Microbiology (Helicobacter Pathogenesis and Immunology. Selected papers)
volume
36
issue
3
pages
141 - 145
publisher
Elsevier
conference name
5th International Workshop on Pathogenesis and Host Response in Helicobacter Infections
external identifiers
  • wos:000182940400005
  • pmid:12738383
  • scopus:0038061766
ISSN
2049-632X
0928-8244
DOI
10.1016/S0928-8244(03)00023-3
language
English
LU publication?
yes
id
7fae56d6-198a-4043-9617-ccc8eb2a2511 (old id 310897)
date added to LUP
2007-09-20 19:03:37
date last changed
2018-05-29 10:05:53
@inproceedings{7fae56d6-198a-4043-9617-ccc8eb2a2511,
  abstract     = {In this study, we assessed the proliferative response of peripheral blood mononuclear leukocytes (PBML) from 33 children/young adolescents with chronic dyspepsia, to H. pylori LPS in the presence and absence of IL-2 as a T cell growth factor. A rapid urease test (RUT) and a presence of Helicobacter-like organisms (HLO) in the biopsy specimens allowed us to distinguish RUT/HLO-positive (17/33) and -negative (16/33) patients. H. pylori LPS alone induced a proliferation of PBML from 4 out of 33 dyspeptic patients. IL-2 increased the prevalence of the response to LPS to 59% and 74% of RUT/HLO-positive and -negative patients, respectively. PBML from RUT/HLO-positive patients responded significantly less intensively to H. pylori LPS in the presence of IL-2, to IL-2 alone and to H. pylori LPS+IL-2. However, there was no difference in PHA-driven proliferation of PBML from the patients of those two groups. A negative correlation between the responsiveness to H. pylori LPS of PBML and occurrence of type B inflammation in gastric mucosa was demonstrated. The results suggest a contribution of H. pylori LPS to an outcome of H. pylori infection. It is speculated that H. pylori LPS by an activation of immunocompetent cells may reduce gastric inflammation, decrease bacterial load and prolong H. pylori infection. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.},
  author       = {Rudnicka, W and Jarosinska, A and Bak-Romaniszyn, L and Moran, A and Planeta-Malecka, I and Wadström, Torkel and Chmiela, M},
  booktitle    = {FEMS Immunology and Medical Microbiology (Helicobacter Pathogenesis and Immunology. Selected papers)},
  issn         = {2049-632X},
  keyword      = {LPS,IL-2,Helicobacter pylori,inflammation,cell proliferation},
  language     = {eng},
  number       = {3},
  pages        = {141--145},
  publisher    = {Elsevier},
  title        = {Helicobacter pylori lipopolysaccharide in the IL-2 milieu activates lymphocytes from dyspeptic children},
  url          = {http://dx.doi.org/10.1016/S0928-8244(03)00023-3},
  volume       = {36},
  year         = {2003},
}