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Vaginal self-sampling without preservative for human papillomavirus testing shows good sensitivity.

Darlin, Lotten LU ; Borgfeldt, Christer LU ; Forslund, Ola LU ; Henic, Emir LU ; Dillner, Joakim LU and Kannisto, Päivi LU (2012) In Journal of Clinical Virology
Abstract
BACKGROUND:

Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits.



OBJECTIVE:

We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing.



STUDY DESIGN:

Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of... (More)
BACKGROUND:

Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits.



OBJECTIVE:

We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing.



STUDY DESIGN:

Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of the stick and put the cap on. Thereafter, the gynaecologist collected a liquid based cytology (LBC) sample. The presence of HPV-types in SS and LBC samples was analysed with PCR and luminex-based typing.



RESULTS:

High-risk-HPV (hr-HPV) DNA was found in 65 of the tested 108 SS (60%; 95% CI 0.50-0.69), whereas LBC found hr-HPV in 64/108 samples (59%; 95% CI 0.49-0.69). The agreement between sampling with SS and LBC was good, kappa value 0.67 (95% CI; 0.53-0.81). The sensitivity for SS with hr-HPV to find HSIL was 81% (95% CI; 67-95%), specificity 49% (95% CI; 37-60%) and the sensitivity for LBC with hr-HPV to find HSIL was 90% (95% CI 80-100%), specificity53% (95% CI; 42-65%).



CONCLUSIONS:

This new vaginal self-sampling method detects hr-HPV-infections with similar sensitivity as a cervical smear taken by a gynaecologist. This self-sampling method is cost-effective and well tolerated, and the kit is suitable for regular mail transport. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Clinical Virology
publisher
Elsevier
external identifiers
  • wos:000312885600011
  • pmid:23017435
  • scopus:84871475409
ISSN
1386-6532
DOI
10.1016/j.jcv.2012.09.002
language
English
LU publication?
yes
id
0e766020-02f2-43a1-b7a7-29ae0ed73db9 (old id 3123577)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23017435?dopt=Abstract
date added to LUP
2012-10-03 20:29:38
date last changed
2017-07-30 04:48:43
@article{0e766020-02f2-43a1-b7a7-29ae0ed73db9,
  abstract     = {BACKGROUND: <br/><br>
Several strategies have been used to reach non-attending women in organized cervical-cancer-screening programs, with varying success. Self-sampling (SS) for HPV is effective for increasing coverage in screening programs, but requires expensive commercial sampling kits. <br/><br>
<br/><br>
OBJECTIVE: <br/><br>
We aimed to evaluate if vaginal SS, without commercial preservatives was adequate for HPV testing. <br/><br>
<br/><br>
STUDY DESIGN: <br/><br>
Women with abnormal cervical smears as determined from the organized screening program were invited to a colposcopy clinic. The 121 women were asked to insert a cotton swab into the vagina and rotate it, put the cotton swab into a sterile cryotube, break the upper part of the stick and put the cap on. Thereafter, the gynaecologist collected a liquid based cytology (LBC) sample. The presence of HPV-types in SS and LBC samples was analysed with PCR and luminex-based typing. <br/><br>
<br/><br>
RESULTS: <br/><br>
High-risk-HPV (hr-HPV) DNA was found in 65 of the tested 108 SS (60%; 95% CI 0.50-0.69), whereas LBC found hr-HPV in 64/108 samples (59%; 95% CI 0.49-0.69). The agreement between sampling with SS and LBC was good, kappa value 0.67 (95% CI; 0.53-0.81). The sensitivity for SS with hr-HPV to find HSIL was 81% (95% CI; 67-95%), specificity 49% (95% CI; 37-60%) and the sensitivity for LBC with hr-HPV to find HSIL was 90% (95% CI 80-100%), specificity53% (95% CI; 42-65%). <br/><br>
<br/><br>
CONCLUSIONS: <br/><br>
This new vaginal self-sampling method detects hr-HPV-infections with similar sensitivity as a cervical smear taken by a gynaecologist. This self-sampling method is cost-effective and well tolerated, and the kit is suitable for regular mail transport.},
  author       = {Darlin, Lotten and Borgfeldt, Christer and Forslund, Ola and Henic, Emir and Dillner, Joakim and Kannisto, Päivi},
  issn         = {1386-6532},
  language     = {eng},
  month        = {09},
  publisher    = {Elsevier},
  series       = {Journal of Clinical Virology},
  title        = {Vaginal self-sampling without preservative for human papillomavirus testing shows good sensitivity.},
  url          = {http://dx.doi.org/10.1016/j.jcv.2012.09.002},
  year         = {2012},
}