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Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins.

Hajizadeh, Solmaz LU ; Kirsebom, Harald LU ; Leistner, Andre and Mattiasson, Bo LU (2012) In Journal of Separation Science 35(21). p.2978-2985
Abstract
Composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. The composites with immobilized concanavalin A (Con A) were used for capturing glycoproteins. Adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. The monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions at sub-zero temperature. The cryogels were epoxy activated and Con A was immobilized as an affinity ligand. Binding and elution of horseradish peroxidase (HRP) was studied in batch experiment and in a chromatographic setup. Increasing adsorbent... (More)
Composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. The composites with immobilized concanavalin A (Con A) were used for capturing glycoproteins. Adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. The monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions at sub-zero temperature. The cryogels were epoxy activated and Con A was immobilized as an affinity ligand. Binding and elution of horseradish peroxidase (HRP) was studied in batch experiment and in a chromatographic setup. Increasing adsorbent concentration in composite cryogels will increase ligand density, which therefore enhances the amount of bound HRP from 0.98 till 2.9 (milligram enzyme per milliliter of gel) in the chromatographic system. The material was evaluated in 10 cycles for binding and elution of HRP. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Separation Science
volume
35
issue
21
pages
2978 - 2985
publisher
John Wiley & Sons
external identifiers
  • wos:000310250300020
  • pmid:23002026
  • scopus:84867856871
ISSN
1615-9314
DOI
10.1002/jssc.201200433
language
English
LU publication?
yes
id
20a6875b-d464-4056-9ea6-3caf3a11c67a (old id 3123693)
date added to LUP
2012-10-10 12:50:23
date last changed
2017-07-09 03:25:12
@article{20a6875b-d464-4056-9ea6-3caf3a11c67a,
  abstract     = {Composite cryogels containing porous adsorbent particles were prepared under cryogelation conditions. The composites with immobilized concanavalin A (Con A) were used for capturing glycoproteins. Adsorbent particles were introduced into the structure in order to improve the capacity and to facilitate the handling of the particles. The monolithic composite cryogels were produced from suspensions of polyvinyl alcohol particles and porous adsorbent particles and cross-linked under acidic conditions at sub-zero temperature. The cryogels were epoxy activated and Con A was immobilized as an affinity ligand. Binding and elution of horseradish peroxidase (HRP) was studied in batch experiment and in a chromatographic setup. Increasing adsorbent concentration in composite cryogels will increase ligand density, which therefore enhances the amount of bound HRP from 0.98 till 2.9 (milligram enzyme per milliliter of gel) in the chromatographic system. The material was evaluated in 10 cycles for binding and elution of HRP.},
  author       = {Hajizadeh, Solmaz and Kirsebom, Harald and Leistner, Andre and Mattiasson, Bo},
  issn         = {1615-9314},
  language     = {eng},
  number       = {21},
  pages        = {2978--2985},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Separation Science},
  title        = {Composite cryogel with immobilized concanavalin A for affinity chromatography of glycoproteins.},
  url          = {http://dx.doi.org/10.1002/jssc.201200433},
  volume       = {35},
  year         = {2012},
}