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A solid state transition in the tetragonal lipid bilayer structure at the lung alveolar surface

Larsson, Marcus LU ; Terasaki, O and Larsson, Kåre LU (2003) In Solid State Sciences 5(1). p.109-114
Abstract
According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung... (More)
According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung surfactant extracts, there are cholesterol-rich regions that remain in the liquid-like disordered conformation at cooling through this transition. The bilayer-embedded proteins in the alveolar CLP; structure are proposed to be located in the "corners" of the CLP-structure. This surface-phase structure was also examined by conventional electron microscopy with fixation at room temperature, and it was found to exhibit quite planar bilayer regions. On the other side, the same-sample seen in cryo-transmission electron microscopy vitrified from 40 degreesC was more disordered. These observations are consistent with a partial solid state transition of the bilayer on cooling, with segregation of lipids within the bilayers into cholesterol-rich regions remaining in a liquid-like disordered state and cholesterol-poor regions crystallizing in the range 25-15 degreesC. Physiological consequences-of such a partial solidification are finally discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
solid/liquid transition, lipid bilayer transition, lung surfactant, tubular myelin
in
Solid State Sciences
volume
5
issue
1
pages
109 - 114
publisher
Editions scientifiques Elsevier
external identifiers
  • wos:000182558200010
  • scopus:0037279623
ISSN
1873-3085
DOI
10.1016/S1293-2558(02)00084-5
language
English
LU publication?
yes
id
6b4cc0da-94db-4570-b5a9-c909f5d3b552 (old id 312372)
date added to LUP
2007-08-03 12:48:22
date last changed
2018-05-29 10:21:59
@article{6b4cc0da-94db-4570-b5a9-c909f5d3b552,
  abstract     = {According to our recent results the alveolar surface is formed by a coherent phase, not a monolayer as has been assumed earlier. This surface phase is a tetragonal organization of the lipid bilayer which seems to follow the CLP minimal surface structure. As lipid bilayers at cooling will undergo a transition from the liquid-crystalline type of structure into a solid state structure, we have followed the changes in the X-ray scattering curves versus temperature of a sample of lung lavage from rabbit. There are significant changes in the range 15-25 degreesC indicating a solid/liquid bilayer transition. The size of the X-ray scattering changes indicate that only parts of the bilayers are involved. As indicated by similar studies of lung surfactant extracts, there are cholesterol-rich regions that remain in the liquid-like disordered conformation at cooling through this transition. The bilayer-embedded proteins in the alveolar CLP; structure are proposed to be located in the "corners" of the CLP-structure. This surface-phase structure was also examined by conventional electron microscopy with fixation at room temperature, and it was found to exhibit quite planar bilayer regions. On the other side, the same-sample seen in cryo-transmission electron microscopy vitrified from 40 degreesC was more disordered. These observations are consistent with a partial solid state transition of the bilayer on cooling, with segregation of lipids within the bilayers into cholesterol-rich regions remaining in a liquid-like disordered state and cholesterol-poor regions crystallizing in the range 25-15 degreesC. Physiological consequences-of such a partial solidification are finally discussed.},
  author       = {Larsson, Marcus and Terasaki, O and Larsson, Kåre},
  issn         = {1873-3085},
  keyword      = {solid/liquid transition,lipid bilayer transition,lung surfactant,tubular myelin},
  language     = {eng},
  number       = {1},
  pages        = {109--114},
  publisher    = {Editions scientifiques Elsevier},
  series       = {Solid State Sciences},
  title        = {A solid state transition in the tetragonal lipid bilayer structure at the lung alveolar surface},
  url          = {http://dx.doi.org/10.1016/S1293-2558(02)00084-5},
  volume       = {5},
  year         = {2003},
}