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HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.

Lavant, Ewa H and Carlson, Joyce LU (2013) In Methods in Molecular Biology 919. p.297-307
Abstract
The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Methods in Molecular Biology
volume
919
pages
297 - 307
publisher
Springer
external identifiers
  • pmid:22976110
  • scopus:84872676975
ISSN
1940-6029
DOI
10.1007/978-1-62703-029-8_26
language
English
LU publication?
yes
id
3e0b0aa7-0273-4afc-b081-b5401fe0d963 (old id 3123960)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22976110?dopt=Abstract
date added to LUP
2012-10-03 21:35:20
date last changed
2018-01-07 09:57:33
@article{3e0b0aa7-0273-4afc-b081-b5401fe0d963,
  abstract     = {The risk for celiac disease (CD) is clearly related to specific HLA DQA1 and DQB1 alleles, but HLA -typing is often considered too costly for frequent use.Here we present a method using sequence-specific primed PCR (PCR-SSP) for HLA-DR-DQ genotyping optimized for capillary electrophoresis on Applied Biosystems 3130xl Genetic Analyzer. Requiring a total of three PCR reactions and a single electrophoretic step, this method reduces the reagent expenses and technical time for directed HLA typing to distinguish risk alleles for CD, with a sufficient throughput for large-scale screening projects.},
  author       = {Lavant, Ewa H and Carlson, Joyce},
  issn         = {1940-6029},
  language     = {eng},
  pages        = {297--307},
  publisher    = {Springer},
  series       = {Methods in Molecular Biology},
  title        = {HLA DR-DQ Genotyping by Capillary Electrophoresis for Risk Assessment for Celiac Disease.},
  url          = {http://dx.doi.org/10.1007/978-1-62703-029-8_26},
  volume       = {919},
  year         = {2013},
}