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Mendelian Inheritance Pattern and High Mutation Rates of Microsatellite Alleles in the Diatom Pseudo-nitzschia multistriata.

Tesson, Sylvie LU ; Legrand, Christophe; van Oosterhout, Cock; Montresor, Marina; Kooistra, Wiebe H C F and Procaccini, Gabriele (2012) In Protist
Abstract
The diatom Pseudo-nitzschia multistriata exhibits a diplontic life cycle composed of an extensive phase of vegetative cell division and a brief phase of sexual reproduction. To explore genotypic stability, we genotyped seven polymorphic microsatellite loci in 26 monoclonal strains over 3-16 months in a culture maintenance regime. Moreover, to assess inheritance patterns of the microsatellite alleles, we genotyped 246 F1 strains resulting from four mating experiments between parental strains of know genotype. Results generally conformed expectations according to Mendelian inheritance patterns, but deviations were detected indicating mutations during sexual reproduction. A total of forty-two mutations were detected in the clonal cultures... (More)
The diatom Pseudo-nitzschia multistriata exhibits a diplontic life cycle composed of an extensive phase of vegetative cell division and a brief phase of sexual reproduction. To explore genotypic stability, we genotyped seven polymorphic microsatellite loci in 26 monoclonal strains over 3-16 months in a culture maintenance regime. Moreover, to assess inheritance patterns of the microsatellite alleles, we genotyped 246 F1 strains resulting from four mating experiments between parental strains of know genotype. Results generally conformed expectations according to Mendelian inheritance patterns, but deviations were detected indicating mutations during sexual reproduction. A total of forty-two mutations were detected in the clonal cultures over time. Microsatellites with more core-repeats accumulated mutations faster. The mutation rate varied significantly across loci and strains. A binomial mass function and a computer simulation showed that the mutation rate was significantly higher during the first months of culture (μ≈3×10(-3) per locus per cell division) and decreased to μ≈1×10(-3) in the strains kept for 16 months. Our results suggest that genetic mutations acquired in both the vegetative phase and sexual reproduction add to the allelic diversity of microsatellites, and hence to the genotypic variation present in a natural population. (Less)
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organization
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Contribution to journal
publication status
published
subject
keywords
diatom, Mendelian inheritance, microsatellites, mutation accumulation lines, mutation rate, Asexual and sexual reproduction
in
Protist
publisher
Elsevier
external identifiers
  • wos:000312188500009
  • pmid:22951213
  • scopus:84872274351
ISSN
1434-4610
DOI
10.1016/j.protis.2012.07.001
language
English
LU publication?
yes
id
d86f33a2-a1b4-49fa-a470-6e595536c919 (old id 3124278)
date added to LUP
2012-10-15 12:57:21
date last changed
2017-07-23 03:29:19
@article{d86f33a2-a1b4-49fa-a470-6e595536c919,
  abstract     = {The diatom Pseudo-nitzschia multistriata exhibits a diplontic life cycle composed of an extensive phase of vegetative cell division and a brief phase of sexual reproduction. To explore genotypic stability, we genotyped seven polymorphic microsatellite loci in 26 monoclonal strains over 3-16 months in a culture maintenance regime. Moreover, to assess inheritance patterns of the microsatellite alleles, we genotyped 246 F1 strains resulting from four mating experiments between parental strains of know genotype. Results generally conformed expectations according to Mendelian inheritance patterns, but deviations were detected indicating mutations during sexual reproduction. A total of forty-two mutations were detected in the clonal cultures over time. Microsatellites with more core-repeats accumulated mutations faster. The mutation rate varied significantly across loci and strains. A binomial mass function and a computer simulation showed that the mutation rate was significantly higher during the first months of culture (μ≈3×10(-3) per locus per cell division) and decreased to μ≈1×10(-3) in the strains kept for 16 months. Our results suggest that genetic mutations acquired in both the vegetative phase and sexual reproduction add to the allelic diversity of microsatellites, and hence to the genotypic variation present in a natural population.},
  author       = {Tesson, Sylvie and Legrand, Christophe and van Oosterhout, Cock and Montresor, Marina and Kooistra, Wiebe H C F and Procaccini, Gabriele},
  issn         = {1434-4610},
  keyword      = {diatom,Mendelian inheritance,microsatellites,mutation accumulation lines,mutation rate,Asexual and sexual reproduction},
  language     = {eng},
  month        = {08},
  publisher    = {Elsevier},
  series       = {Protist},
  title        = {Mendelian Inheritance Pattern and High Mutation Rates of Microsatellite Alleles in the Diatom Pseudo-nitzschia multistriata.},
  url          = {http://dx.doi.org/10.1016/j.protis.2012.07.001},
  year         = {2012},
}