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Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.

Kondepudi, Kanthi Kiran; Ambalam, Padma; Nilsson, Ingrid LU ; Wadström, Torkel LU and Ljungh, Åsa LU (2012) In Anaerobe 18(5). p.489-497
Abstract
Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and... (More)
Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and lactulose as carbon sources and moderate degradation of fructooligosaccharides (FOS). Xylooligosaccharides (XOS) was metabolized to a greater extent by B. lactis 8:8, B. lactis JCM 10602, B. pseudocatenulatum JCM 1200 and B. longum 6:18 (prebiotic score >50%). All strains exhibited extracellular antimicrobial activity (AMA) against four CD strains including the CD NAP1/027. AMA of B. breve 46, B. lactis 8:8 and B. lactis JCM 10602 strains was mainly ascribed to a combined action of organic acids and heat stable, protease sensitive antimicrobial peptides when cells were grown in MRSC broth with glucose and by acids when grown with five different prebiotic-non-digestible oligosaccharides (NDOs). None of C. difficile strains degraded five prebiotic-NDOs. Whole cells of B. breve 46 and B. lactis 8:8 and their supernatants inhibited the growth and toxin production of the CD NAP1/027 strain. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Anaerobe
volume
18
issue
5
pages
489 - 497
publisher
Elsevier
external identifiers
  • wos:000310943200003
  • pmid:22940065
  • scopus:84867329333
ISSN
1095-8274
DOI
10.1016/j.anaerobe.2012.08.005
language
English
LU publication?
yes
id
f7e2fb52-6cd9-4047-b25f-fd9d52e4b9fe (old id 3124479)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22940065?dopt=Abstract
date added to LUP
2012-10-03 11:29:34
date last changed
2017-10-29 04:27:07
@article{f7e2fb52-6cd9-4047-b25f-fd9d52e4b9fe,
  abstract     = {Bifidobacterium breve 46, Bifidobacteriumlactis 8:8 and Bifidobacteriumlongum 6:18 and three reference strains B. breve CCUG 24611, B. lactis JCM 10602, and Bifidobacteriumpseudocatenulatum JCM 1200 were examined for acid and bile tolerance, prebiotic utilization and antimicrobial activity against four Clostridium difficile (CD) strains including the hypervirulent strain, PCR ribotype NAP1/027. B. lactis 8:8 and B. lactis JCM 10602 exhibited a high tolerance in MRSC broth with pH 2.5 for 30 min. B. breve 46 and B. lactis 8:8 remained 100% viable in MRSC broth with 5% porcine bile after 4 h. All six strains showed a high prebiotic degrading ability (prebiotic score) with galactooligosaccharides (GOS), isomaltooligosaccharides (IMOS) and lactulose as carbon sources and moderate degradation of fructooligosaccharides (FOS). Xylooligosaccharides (XOS) was metabolized to a greater extent by B. lactis 8:8, B. lactis JCM 10602, B. pseudocatenulatum JCM 1200 and B. longum 6:18 (prebiotic score >50%). All strains exhibited extracellular antimicrobial activity (AMA) against four CD strains including the CD NAP1/027. AMA of B. breve 46, B. lactis 8:8 and B. lactis JCM 10602 strains was mainly ascribed to a combined action of organic acids and heat stable, protease sensitive antimicrobial peptides when cells were grown in MRSC broth with glucose and by acids when grown with five different prebiotic-non-digestible oligosaccharides (NDOs). None of C. difficile strains degraded five prebiotic-NDOs. Whole cells of B. breve 46 and B. lactis 8:8 and their supernatants inhibited the growth and toxin production of the CD NAP1/027 strain.},
  author       = {Kondepudi, Kanthi Kiran and Ambalam, Padma and Nilsson, Ingrid and Wadström, Torkel and Ljungh, Åsa},
  issn         = {1095-8274},
  language     = {eng},
  number       = {5},
  pages        = {489--497},
  publisher    = {Elsevier},
  series       = {Anaerobe},
  title        = {Prebiotic-non-digestible oligosaccharides preference of probiotic bifidobacteria and antimicrobial activity against Clostridium difficile.},
  url          = {http://dx.doi.org/10.1016/j.anaerobe.2012.08.005},
  volume       = {18},
  year         = {2012},
}