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Involvement of alpha 7 beta 1 integrin in the conditioning-lesion effect on sensory axon regeneration

Ekström, Per LU ; Mayer, U; Panjwani, A; Pountney, D; Pizzey, J and Tonge, DA (2003) In Molecular and Cellular Neuroscience 22(3). p.383-395
Abstract
Conditioning lesions of peripheral nerves improve axonal regeneration after injury and involve changes in expression of proteins required for axonal growth. Integrin alpha7beta1 expression in motor and sensory neurons increases following nerve lesions and motor axon regeneration is impaired in alpha7 integrin KO mice (J. Neurosci. 20, 1822-1830). To investigate the role of alpha7beta1 integrin in sensory axon regeneration, dorsal root ganglia of adult mice were cultured in gels of laminin-rich extracellular matrix (Matrigel) or collagen. Normal dorsal root ganglia in Matrigel or collagen supplemented with laminin showed spontaneous axonal outgrowth, which was greatly increased in conditioned preparations, but only in the presence of... (More)
Conditioning lesions of peripheral nerves improve axonal regeneration after injury and involve changes in expression of proteins required for axonal growth. Integrin alpha7beta1 expression in motor and sensory neurons increases following nerve lesions and motor axon regeneration is impaired in alpha7 integrin KO mice (J. Neurosci. 20, 1822-1830). To investigate the role of alpha7beta1 integrin in sensory axon regeneration, dorsal root ganglia of adult mice were cultured in gels of laminin-rich extracellular matrix (Matrigel) or collagen. Normal dorsal root ganglia in Matrigel or collagen supplemented with laminin showed spontaneous axonal outgrowth, which was greatly increased in conditioned preparations, but only in the presence of laminin. Conditioned dorsal root ganglia from normal mice cultured with a blocking antibody to beta1 integrin and from alpha7 integrin KO mice showed reduced axonal growth in both Matrigel- and laminin-supplemented collagen gels. Enhanced axonal regeneration after conditioning lesions therefore involves increased responsiveness to laminin and integrin alpha7beta1 expression. (C) 2003 Elsevier Science (USA). All rights reserved. (Less)
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Contribution to journal
publication status
published
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in
Molecular and Cellular Neuroscience
volume
22
issue
3
pages
383 - 395
publisher
Elsevier
external identifiers
  • wos:000182313600009
  • scopus:0037353787
ISSN
1044-7431
DOI
language
English
LU publication?
yes
id
aba0acd3-9050-49c4-abef-2f883f04ec54 (old id 312964)
date added to LUP
2007-08-29 14:55:52
date last changed
2018-05-29 10:51:56
@article{aba0acd3-9050-49c4-abef-2f883f04ec54,
  abstract     = {Conditioning lesions of peripheral nerves improve axonal regeneration after injury and involve changes in expression of proteins required for axonal growth. Integrin alpha7beta1 expression in motor and sensory neurons increases following nerve lesions and motor axon regeneration is impaired in alpha7 integrin KO mice (J. Neurosci. 20, 1822-1830). To investigate the role of alpha7beta1 integrin in sensory axon regeneration, dorsal root ganglia of adult mice were cultured in gels of laminin-rich extracellular matrix (Matrigel) or collagen. Normal dorsal root ganglia in Matrigel or collagen supplemented with laminin showed spontaneous axonal outgrowth, which was greatly increased in conditioned preparations, but only in the presence of laminin. Conditioned dorsal root ganglia from normal mice cultured with a blocking antibody to beta1 integrin and from alpha7 integrin KO mice showed reduced axonal growth in both Matrigel- and laminin-supplemented collagen gels. Enhanced axonal regeneration after conditioning lesions therefore involves increased responsiveness to laminin and integrin alpha7beta1 expression. (C) 2003 Elsevier Science (USA). All rights reserved.},
  author       = {Ekström, Per and Mayer, U and Panjwani, A and Pountney, D and Pizzey, J and Tonge, DA},
  issn         = {1044-7431},
  language     = {eng},
  number       = {3},
  pages        = {383--395},
  publisher    = {Elsevier},
  series       = {Molecular and Cellular Neuroscience},
  title        = {Involvement of alpha 7 beta 1 integrin in the conditioning-lesion effect on sensory axon regeneration},
  url          = {http://dx.doi.org/},
  volume       = {22},
  year         = {2003},
}