Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

SERS mapping in langmuir-blodgett films and single-molecule detection

Aoki, Pedro H.B. ; Carreon, Eduardo G.E. ; Volpati, Diogo LU ; Shimabukuro, Milton H. ; Constantino, Carlos J.L. ; Aroca, Ricardo F. ; Oliveira, Osvaldo N. and Paulovich, Fernando V. (2013) In Applied Spectroscopy 67(5). p.563-569
Abstract

Plasmon-enhanced spectroscopic techniques have expanded single-molecule detection (SMD) and are revolutionizing areas such as bio-imaging and single-cell manipulation. Surface-enhanced (resonance) Raman scattering (SERS or SERRS) combines high sensitivity with molecularfingerprint information at the single-molecule level. Spectra originating from single-molecule SERS experiments are rare events, which occur only if a single molecule is located in a hot-spot zone. In this spot, the molecule is selectively exposed to a significant enhancement associated with a high, local electromagnetic field in the plasmonic substrate. Here, we report an SMD study with an electrostatic approach in which a Langmuir film of a phospholipid with anionic... (More)

Plasmon-enhanced spectroscopic techniques have expanded single-molecule detection (SMD) and are revolutionizing areas such as bio-imaging and single-cell manipulation. Surface-enhanced (resonance) Raman scattering (SERS or SERRS) combines high sensitivity with molecularfingerprint information at the single-molecule level. Spectra originating from single-molecule SERS experiments are rare events, which occur only if a single molecule is located in a hot-spot zone. In this spot, the molecule is selectively exposed to a significant enhancement associated with a high, local electromagnetic field in the plasmonic substrate. Here, we report an SMD study with an electrostatic approach in which a Langmuir film of a phospholipid with anionic polar head groups (PO 4 -) was doped with cationic methylene blue (MB), creating a homogeneous, two-dimensional distribution of dyes in the monolayer. The number of dyes in the probed area of the Langmuir-Blodgett (LB) film coating the Ag nanostructures established a regime in which single-molecule events were observed, with the identification based on direct matching of the observed spectrum at each point of the mapping with a reference spectrum for the MB molecule. In addition, advanced fitting techniques were tested with the data obtained from micro-Raman mapping, thus achieving real-time processing to extract the MB single-molecule spectra.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
SERS, Single-molecule detection (SMD), Visual data exploration
in
Applied Spectroscopy
volume
67
issue
5
pages
7 pages
publisher
Society for Applied Spectroscopy
external identifiers
  • scopus:84880049223
ISSN
0003-7028
DOI
10.1366/12-06909
language
English
LU publication?
no
id
312d2c4b-9b1f-409c-91e1-bb245a445957
date added to LUP
2019-05-17 14:36:05
date last changed
2022-03-18 00:04:44
@article{312d2c4b-9b1f-409c-91e1-bb245a445957,
  abstract     = {{<p>Plasmon-enhanced spectroscopic techniques have expanded single-molecule detection (SMD) and are revolutionizing areas such as bio-imaging and single-cell manipulation. Surface-enhanced (resonance) Raman scattering (SERS or SERRS) combines high sensitivity with molecularfingerprint information at the single-molecule level. Spectra originating from single-molecule SERS experiments are rare events, which occur only if a single molecule is located in a hot-spot zone. In this spot, the molecule is selectively exposed to a significant enhancement associated with a high, local electromagnetic field in the plasmonic substrate. Here, we report an SMD study with an electrostatic approach in which a Langmuir film of a phospholipid with anionic polar head groups (PO <sub>4</sub> <sup>-</sup>) was doped with cationic methylene blue (MB), creating a homogeneous, two-dimensional distribution of dyes in the monolayer. The number of dyes in the probed area of the Langmuir-Blodgett (LB) film coating the Ag nanostructures established a regime in which single-molecule events were observed, with the identification based on direct matching of the observed spectrum at each point of the mapping with a reference spectrum for the MB molecule. In addition, advanced fitting techniques were tested with the data obtained from micro-Raman mapping, thus achieving real-time processing to extract the MB single-molecule spectra.</p>}},
  author       = {{Aoki, Pedro H.B. and Carreon, Eduardo G.E. and Volpati, Diogo and Shimabukuro, Milton H. and Constantino, Carlos J.L. and Aroca, Ricardo F. and Oliveira, Osvaldo N. and Paulovich, Fernando V.}},
  issn         = {{0003-7028}},
  keywords     = {{SERS; Single-molecule detection (SMD); Visual data exploration}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{5}},
  pages        = {{563--569}},
  publisher    = {{Society for Applied Spectroscopy}},
  series       = {{Applied Spectroscopy}},
  title        = {{SERS mapping in langmuir-blodgett films and single-molecule detection}},
  url          = {{http://dx.doi.org/10.1366/12-06909}},
  doi          = {{10.1366/12-06909}},
  volume       = {{67}},
  year         = {{2013}},
}