The plasma concentration of HDL-associated apoM is influenced by LDL receptor-mediated clearance of apoB-containing particles
(2012) In Journal of Lipid Research 53(10). p.2198-2204- Abstract
- ApoM is mainly associated with HDL. Nevertheless, we have consistently observed positive correlations of apoM with plasma LDL cholesterol in humans. Moreover, LDL receptor deficiency is associated with increased plasma apoM in mice. Here, we tested the idea that plasma apoM concentrations are affected by the rate of LDL receptor-mediated clearance of apoB-containing particles. We measured apoM in humans each carrying one of three different LDL receptor mutations (n = 9) or the apoB3500 mutation (n = 12). These carriers had increased plasma apoM (1.34 +/- 0.13 mu M, P = 0.003, and 1.23 +/- 0.10 mu M, P = 0.02, respectively) as compared with noncarriers (0.93 +/- 0.04 mu M). When we injected human apoM-containing HDL into Wt (n = 6) or LDL... (More)
- ApoM is mainly associated with HDL. Nevertheless, we have consistently observed positive correlations of apoM with plasma LDL cholesterol in humans. Moreover, LDL receptor deficiency is associated with increased plasma apoM in mice. Here, we tested the idea that plasma apoM concentrations are affected by the rate of LDL receptor-mediated clearance of apoB-containing particles. We measured apoM in humans each carrying one of three different LDL receptor mutations (n = 9) or the apoB3500 mutation (n = 12). These carriers had increased plasma apoM (1.34 +/- 0.13 mu M, P = 0.003, and 1.23 +/- 0.10 mu M, P = 0.02, respectively) as compared with noncarriers (0.93 +/- 0.04 mu M). When we injected human apoM-containing HDL into Wt (n = 6) or LDL receptor-deficient mice (n = 6), the removal of HDL-associated human apoM was delayed in the LDL receptor-deficient mice. After 2 h, 54 +/- 5% versus 90 +/- 8% (P < 0.005) of the initial amounts of human apoM remained in the plasma of Wt and LDL receptor-deficient mice, respectively. Finally, we compared the turnover of radio-iodinated LDL and plasma apoM concentrations in 45 normocholesterolemic humans. There was a negative correlation between plasma apoM and the fractional catabolic rate of LDL (r = -0.38, P = 0.009). These data suggest that the plasma clearance of apoM, despite apoM primarily being associated with HDL, is influenced by LDL receptor-mediated clearance of apoB-containing particles.-Christoffersen, C., M. Benn, P. M. Christensen, P. L. S. M. Gordts, A. J. M. Roebroek, R. Frikke-Schmidt, A. Tybjaerg-Hansen, B. Dahlback, and L. B. Nielsen. The plasma concentration of HDL-associated apoM is influenced by LDL receptor-mediated clearance of apoB-containing particles. J. Lipid Res. 2012. 53: 2198-2204. (Less)
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https://lup.lub.lu.se/record/3135932
- author
- Christoffersen, Christina ; Benn, Marianne ; Christensen, Pernille M. ; Gordts, Philip L. S. M. ; Roebroek, Anton J. M. ; Frikke-Schmidt, Ruth ; Tybjaerg-Hansen, Anne ; Dahlbäck, Björn LU and Nielsen, Lars B.
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lipoprotein, low-density lipoprotein metabolism, apolipoprotein, familial hypercholesterolemia
- in
- Journal of Lipid Research
- volume
- 53
- issue
- 10
- pages
- 2198 - 2204
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- wos:000308696600019
- scopus:84866178749
- pmid:22826357
- ISSN
- 1539-7262
- DOI
- 10.1194/jlr.P023697
- language
- English
- LU publication?
- yes
- id
- dd36f218-6658-46a5-a349-332cf085954d (old id 3135932)
- date added to LUP
- 2016-04-01 10:47:25
- date last changed
- 2022-01-26 02:32:29
@article{dd36f218-6658-46a5-a349-332cf085954d, abstract = {{ApoM is mainly associated with HDL. Nevertheless, we have consistently observed positive correlations of apoM with plasma LDL cholesterol in humans. Moreover, LDL receptor deficiency is associated with increased plasma apoM in mice. Here, we tested the idea that plasma apoM concentrations are affected by the rate of LDL receptor-mediated clearance of apoB-containing particles. We measured apoM in humans each carrying one of three different LDL receptor mutations (n = 9) or the apoB3500 mutation (n = 12). These carriers had increased plasma apoM (1.34 +/- 0.13 mu M, P = 0.003, and 1.23 +/- 0.10 mu M, P = 0.02, respectively) as compared with noncarriers (0.93 +/- 0.04 mu M). When we injected human apoM-containing HDL into Wt (n = 6) or LDL receptor-deficient mice (n = 6), the removal of HDL-associated human apoM was delayed in the LDL receptor-deficient mice. After 2 h, 54 +/- 5% versus 90 +/- 8% (P < 0.005) of the initial amounts of human apoM remained in the plasma of Wt and LDL receptor-deficient mice, respectively. Finally, we compared the turnover of radio-iodinated LDL and plasma apoM concentrations in 45 normocholesterolemic humans. There was a negative correlation between plasma apoM and the fractional catabolic rate of LDL (r = -0.38, P = 0.009). These data suggest that the plasma clearance of apoM, despite apoM primarily being associated with HDL, is influenced by LDL receptor-mediated clearance of apoB-containing particles.-Christoffersen, C., M. Benn, P. M. Christensen, P. L. S. M. Gordts, A. J. M. Roebroek, R. Frikke-Schmidt, A. Tybjaerg-Hansen, B. Dahlback, and L. B. Nielsen. The plasma concentration of HDL-associated apoM is influenced by LDL receptor-mediated clearance of apoB-containing particles. J. Lipid Res. 2012. 53: 2198-2204.}}, author = {{Christoffersen, Christina and Benn, Marianne and Christensen, Pernille M. and Gordts, Philip L. S. M. and Roebroek, Anton J. M. and Frikke-Schmidt, Ruth and Tybjaerg-Hansen, Anne and Dahlbäck, Björn and Nielsen, Lars B.}}, issn = {{1539-7262}}, keywords = {{lipoprotein; low-density lipoprotein metabolism; apolipoprotein; familial hypercholesterolemia}}, language = {{eng}}, number = {{10}}, pages = {{2198--2204}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Lipid Research}}, title = {{The plasma concentration of HDL-associated apoM is influenced by LDL receptor-mediated clearance of apoB-containing particles}}, url = {{http://dx.doi.org/10.1194/jlr.P023697}}, doi = {{10.1194/jlr.P023697}}, volume = {{53}}, year = {{2012}}, }