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Gene expression profiling indicates that immunohistochemical expression of CD40 is a marker of an inflammatory reaction in the tumor stroma of diffuse large B-cell lymphoma

Fjordén, Karin LU ; Joost, Patrick; Ehinger, Mats LU ; Edén, Patrik LU ; Jerkeman, Mats LU ; Cavallin-Ståhl, Eva LU and Linderoth, Johan LU (2012) In Leukemia & Lymphoma 53(9). p.1764-1768
Abstract
Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p... (More)
Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p = 0.005), which in turn correlates with the amount of infiltrating macrophages and CD4 and CD8 positive T-cells. We postulate that immunohistochemical expression of CD40 mainly reflects the inflammatory status in tumors. A high intratumoral inflammatory reaction may correlate with an increased autologous tumor response, and thereby a better prognosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Diffuse large B-cell lymphoma, CD40, gene expression profiling, tumor, stroma
in
Leukemia & Lymphoma
volume
53
issue
9
pages
1764 - 1768
publisher
Taylor & Francis
external identifiers
  • wos:000307301500022
  • scopus:84864988511
ISSN
1042-8194
DOI
10.3109/10428194.2012.666541
language
English
LU publication?
yes
id
8b22050a-bd37-46ce-8265-680d3b720805 (old id 3147755)
date added to LUP
2012-10-31 21:30:24
date last changed
2017-02-05 04:07:19
@article{8b22050a-bd37-46ce-8265-680d3b720805,
  abstract     = {Immunohistochemical expression of CD40 is seen in 60-70% of diffuse large B-cell lymphoma (DLBCL) and is associated with a superior prognosis. By using gene expression profiling we aimed to further explore the underlying mechanisms for this effect. Ninety-eight immunohistochemically defined CD40 positive or negative DLBCL tumors, 63 and 35 respectively, were examined using spotted 55K oligonucleotide arrays. CD40 expressing tumors were characterized by up-regulated expression of genes encoding proteins involved in cell-matrix interactions: collagens, integrin a V, proteoglycans and proteolytic enzymes, and antigen presentation. Immunohistochemistry confirmed that CD40 positive tumors co-express the proinflammatory proteoglycan biglycan (p = 0.005), which in turn correlates with the amount of infiltrating macrophages and CD4 and CD8 positive T-cells. We postulate that immunohistochemical expression of CD40 mainly reflects the inflammatory status in tumors. A high intratumoral inflammatory reaction may correlate with an increased autologous tumor response, and thereby a better prognosis.},
  author       = {Fjordén, Karin and Joost, Patrick and Ehinger, Mats and Edén, Patrik and Jerkeman, Mats and Cavallin-Ståhl, Eva and Linderoth, Johan},
  issn         = {1042-8194},
  keyword      = {Diffuse large B-cell lymphoma,CD40,gene expression profiling,tumor,stroma},
  language     = {eng},
  number       = {9},
  pages        = {1764--1768},
  publisher    = {Taylor & Francis},
  series       = {Leukemia & Lymphoma},
  title        = {Gene expression profiling indicates that immunohistochemical expression of CD40 is a marker of an inflammatory reaction in the tumor stroma of diffuse large B-cell lymphoma},
  url          = {http://dx.doi.org/10.3109/10428194.2012.666541},
  volume       = {53},
  year         = {2012},
}