ACOUSTOFLUIDIC METHOD TO ALIGN POLYSTYRENE BEADS AND CELLS IN HYDROGEL DROPLETS
(2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.887-888- Abstract
We present a microfluidic system to control the position of particles (polystyrene beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves. Droplets comprising hydrogel precursor solution, photoinitiator and (bio-)particles were generated and acoustic forces focused the beads to the droplet center line. Droplets were cross-linked by exposure to UV-light. With the acoustics applied, 89 ± 19% of the particles were positioned in the center of the hydrogel droplet. As proof-of-principle for biological applications, astrocytes were focused in hydrogel droplets. The viability of the cells after 7 days was unaffected by the acoustic focusing (72 ± 22%).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3153c693-729e-4547-8f90-a7a0c1003aa5
- author
- Pohlit, Hannah ; Fornell, Anna LU ; Shi, Qian and Tenje, Maria LU
- organization
- publishing date
- 2021
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Acoustic Focusing, Cell Encapsulation, Hydrogel Droplet, Microfluidics
- host publication
- MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- pages
- 2 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
- conference location
- Palm Springs, Virtual, United States
- conference dates
- 2021-10-10 - 2021-10-14
- external identifiers
-
- scopus:85136984255
- ISBN
- 9781733419031
- language
- English
- LU publication?
- yes
- id
- 3153c693-729e-4547-8f90-a7a0c1003aa5
- date added to LUP
- 2022-11-08 13:11:16
- date last changed
- 2022-11-09 09:36:31
@inproceedings{3153c693-729e-4547-8f90-a7a0c1003aa5, abstract = {{<p>We present a microfluidic system to control the position of particles (polystyrene beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves. Droplets comprising hydrogel precursor solution, photoinitiator and (bio-)particles were generated and acoustic forces focused the beads to the droplet center line. Droplets were cross-linked by exposure to UV-light. With the acoustics applied, 89 ± 19% of the particles were positioned in the center of the hydrogel droplet. As proof-of-principle for biological applications, astrocytes were focused in hydrogel droplets. The viability of the cells after 7 days was unaffected by the acoustic focusing (72 ± 22%).</p>}}, author = {{Pohlit, Hannah and Fornell, Anna and Shi, Qian and Tenje, Maria}}, booktitle = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}}, isbn = {{9781733419031}}, keywords = {{Acoustic Focusing; Cell Encapsulation; Hydrogel Droplet; Microfluidics}}, language = {{eng}}, pages = {{887--888}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{ACOUSTOFLUIDIC METHOD TO ALIGN POLYSTYRENE BEADS AND CELLS IN HYDROGEL DROPLETS}}, year = {{2021}}, }