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ACOUSTOFLUIDIC METHOD TO ALIGN POLYSTYRENE BEADS AND CELLS IN HYDROGEL DROPLETS

Pohlit, Hannah ; Fornell, Anna LU ; Shi, Qian and Tenje, Maria LU (2021) 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 p.887-888
Abstract

We present a microfluidic system to control the position of particles (polystyrene beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves. Droplets comprising hydrogel precursor solution, photoinitiator and (bio-)particles were generated and acoustic forces focused the beads to the droplet center line. Droplets were cross-linked by exposure to UV-light. With the acoustics applied, 89 ± 19% of the particles were positioned in the center of the hydrogel droplet. As proof-of-principle for biological applications, astrocytes were focused in hydrogel droplets. The viability of the cells after 7 days was unaffected by the acoustic focusing (72 ± 22%).

Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Acoustic Focusing, Cell Encapsulation, Hydrogel Droplet, Microfluidics
host publication
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
pages
2 pages
publisher
Chemical and Biological Microsystems Society
conference name
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
conference location
Palm Springs, Virtual, United States
conference dates
2021-10-10 - 2021-10-14
external identifiers
  • scopus:85136984255
ISBN
9781733419031
language
English
LU publication?
yes
id
3153c693-729e-4547-8f90-a7a0c1003aa5
date added to LUP
2022-11-08 13:11:16
date last changed
2022-11-09 09:36:31
@inproceedings{3153c693-729e-4547-8f90-a7a0c1003aa5,
  abstract     = {{<p>We present a microfluidic system to control the position of particles (polystyrene beads or astrocyte cells) in hydrogel droplets using bulk acoustic standing waves. Droplets comprising hydrogel precursor solution, photoinitiator and (bio-)particles were generated and acoustic forces focused the beads to the droplet center line. Droplets were cross-linked by exposure to UV-light. With the acoustics applied, 89 ± 19% of the particles were positioned in the center of the hydrogel droplet. As proof-of-principle for biological applications, astrocytes were focused in hydrogel droplets. The viability of the cells after 7 days was unaffected by the acoustic focusing (72 ± 22%).</p>}},
  author       = {{Pohlit, Hannah and Fornell, Anna and Shi, Qian and Tenje, Maria}},
  booktitle    = {{MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences}},
  isbn         = {{9781733419031}},
  keywords     = {{Acoustic Focusing; Cell Encapsulation; Hydrogel Droplet; Microfluidics}},
  language     = {{eng}},
  pages        = {{887--888}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  title        = {{ACOUSTOFLUIDIC METHOD TO ALIGN POLYSTYRENE BEADS AND CELLS IN HYDROGEL DROPLETS}},
  year         = {{2021}},
}