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Morphology phenotype and ultrastructure of fibroblastic cells from normal and neuropathic human detrusor: Absence of myofibroblast characteristics

Drake, MJ; Hedlund, Petter LU ; Andersson, Karl-Erik LU ; Brading, AF; Hussain, I; Fowler, C and Landon, DN (2003) In Journal of Urology 169(4). p.1573-1576
Abstract
Purpose: Fibroblasts are functionally diverse and fibroblastic cells with smooth muscle-like characteristics (myofibroblasts) regulate smooth muscle activity in certain tissues. The presence of myofibroblasts has been reported in the bladder with important implications for normal function and detrusor overactivity. We assessed fibroblastic cell characteristics to discern features suggesting a myofibroblast phenotype in normal or neuropathic human detrusor. Materials and Methods: A total of 25 control samples were obtained from cadaveric organ donors or patients with a mean age of 42.3 years investigated for hematuria and compared with samples from 18 patients with a mean age of 37.4 years with neurogenic detrusor overactivity. Morphology,... (More)
Purpose: Fibroblasts are functionally diverse and fibroblastic cells with smooth muscle-like characteristics (myofibroblasts) regulate smooth muscle activity in certain tissues. The presence of myofibroblasts has been reported in the bladder with important implications for normal function and detrusor overactivity. We assessed fibroblastic cell characteristics to discern features suggesting a myofibroblast phenotype in normal or neuropathic human detrusor. Materials and Methods: A total of 25 control samples were obtained from cadaveric organ donors or patients with a mean age of 42.3 years investigated for hematuria and compared with samples from 18 patients with a mean age of 37.4 years with neurogenic detrusor overactivity. Morphology, phenotypic expression of various markers and the ultrastructure of each fibroblastic cell visible in multiple sections from each specimen was evaluated by 2 independent assessors. Results: Fibroblastic cells were observed throughout the smooth muscle and connective tissue. They were located peripherally on muscle fascicles and had a polar stellate appearance with processes ramifying in interfascicular planes and muscle. They possessed vimentin-like immunoreactivity and weak c-kit-like immunoreactivity but not desmin or a-smooth muscle actin-like immunoreactivity. Ultrastructurally they showed dilated rough endoplasmic reticulum with a moderately electron dense amorphous content and prominent golgi complexes. Nuclei had clumped peripheral heterochromatin. There were extensive flattened processes that lacked basal laminae. There was no specific contact with nerve fibers or smooth muscle. Neuropathic bladder samples did not differ overtly from those of controls. Conclusions: The detrusor possesses an extensive network of fibroblastic cells and processes. No evidence of myofibroblast differentiation was discerned in normal or neuropathic detrusor, although a minority subpopulation or regional variability in cellular phenotype could not be excluded. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phenotype, neuropathic, bladder, fibroblasts, smooth, muscle
in
Journal of Urology
volume
169
issue
4
pages
1573 - 1576
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000181639700096
  • pmid:12629418
  • scopus:0037375989
ISSN
1527-3792
DOI
10.1097/01.ju.0000054928.34777.37
language
English
LU publication?
yes
id
0ec30046-fd53-43a7-9512-11e14c3d3a0d (old id 315708)
date added to LUP
2007-08-29 12:46:06
date last changed
2018-01-07 08:46:15
@article{0ec30046-fd53-43a7-9512-11e14c3d3a0d,
  abstract     = {Purpose: Fibroblasts are functionally diverse and fibroblastic cells with smooth muscle-like characteristics (myofibroblasts) regulate smooth muscle activity in certain tissues. The presence of myofibroblasts has been reported in the bladder with important implications for normal function and detrusor overactivity. We assessed fibroblastic cell characteristics to discern features suggesting a myofibroblast phenotype in normal or neuropathic human detrusor. Materials and Methods: A total of 25 control samples were obtained from cadaveric organ donors or patients with a mean age of 42.3 years investigated for hematuria and compared with samples from 18 patients with a mean age of 37.4 years with neurogenic detrusor overactivity. Morphology, phenotypic expression of various markers and the ultrastructure of each fibroblastic cell visible in multiple sections from each specimen was evaluated by 2 independent assessors. Results: Fibroblastic cells were observed throughout the smooth muscle and connective tissue. They were located peripherally on muscle fascicles and had a polar stellate appearance with processes ramifying in interfascicular planes and muscle. They possessed vimentin-like immunoreactivity and weak c-kit-like immunoreactivity but not desmin or a-smooth muscle actin-like immunoreactivity. Ultrastructurally they showed dilated rough endoplasmic reticulum with a moderately electron dense amorphous content and prominent golgi complexes. Nuclei had clumped peripheral heterochromatin. There were extensive flattened processes that lacked basal laminae. There was no specific contact with nerve fibers or smooth muscle. Neuropathic bladder samples did not differ overtly from those of controls. Conclusions: The detrusor possesses an extensive network of fibroblastic cells and processes. No evidence of myofibroblast differentiation was discerned in normal or neuropathic detrusor, although a minority subpopulation or regional variability in cellular phenotype could not be excluded.},
  author       = {Drake, MJ and Hedlund, Petter and Andersson, Karl-Erik and Brading, AF and Hussain, I and Fowler, C and Landon, DN},
  issn         = {1527-3792},
  keyword      = {phenotype,neuropathic,bladder,fibroblasts,smooth,muscle},
  language     = {eng},
  number       = {4},
  pages        = {1573--1576},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Journal of Urology},
  title        = {Morphology phenotype and ultrastructure of fibroblastic cells from normal and neuropathic human detrusor: Absence of myofibroblast characteristics},
  url          = {http://dx.doi.org/10.1097/01.ju.0000054928.34777.37},
  volume       = {169},
  year         = {2003},
}