Immobilisation of DNA to polymerised SU-8 photoresist
(2006) In Biosensors & Bioelectronics 21(7). p.1327-1332- Abstract
- SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA... (More)
- SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3157952
- author
- Marie, Rodolphe ; Schmid, Silvan ; Johansson, Alicia ; Ejsing, Louise ; Nordström, Maria LU ; Häfliger, Daniel ; Christensen, Claus BV ; Boisen, Anja and Dufva, Martin
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biosensors & Bioelectronics
- volume
- 21
- issue
- 7
- pages
- 1327 - 1332
- publisher
- Elsevier
- external identifiers
-
- scopus:29144452883
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2005.03.004
- language
- English
- LU publication?
- no
- id
- f7a21e43-f6e7-4439-8287-245df9389a19 (old id 3157952)
- date added to LUP
- 2016-04-01 16:51:48
- date last changed
- 2022-01-28 22:39:31
@article{f7a21e43-f6e7-4439-8287-245df9389a19, abstract = {{SU-8 is an epoxy-based photosensitive resist, which is currently used for a large variety of MEMS and lab-on-a-chip applications. Here, we demonstrate a one-step process to functionalise SU-8 with DNA probes. The immobilisation procedure relies on direct coupling of DNA to SU-8 and resulted in surfaces with functional capture probe densities of approximately 10 fmol/mm2 as determined by hybridisation assays with fluorescent labelled target molecules. A comparable density of functional capture probes was measured on commercial aldehyde coated glass. DNA probes did not decrease in hybridisation performance after 10 min incubation in water at 98 °C prior to hybridisation, indicating a covalent bond between DNA and SU-8. Finally, DNA microarrays of high quality were obtained on SU-8 by contact printing of probe solution directly on SU-8 demonstrating a simple method for the implementation of microarrays in microsystems.}}, author = {{Marie, Rodolphe and Schmid, Silvan and Johansson, Alicia and Ejsing, Louise and Nordström, Maria and Häfliger, Daniel and Christensen, Claus BV and Boisen, Anja and Dufva, Martin}}, issn = {{1873-4235}}, language = {{eng}}, number = {{7}}, pages = {{1327--1332}}, publisher = {{Elsevier}}, series = {{Biosensors & Bioelectronics}}, title = {{Immobilisation of DNA to polymerised SU-8 photoresist}}, url = {{http://dx.doi.org/10.1016/j.bios.2005.03.004}}, doi = {{10.1016/j.bios.2005.03.004}}, volume = {{21}}, year = {{2006}}, }