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Functional association of cytokine-induced SH2 protein and protein kinase C in activated T cells

Chen, S; Anderson, Per LU ; Li, Li LU ; Sjögren, Hans Olov LU ; Wang, P and Li, Su-Ling LU (2003) In International Immunology 15(3). p.403-409
Abstract
TCR signaling is mediated by intracellular signaling molecules and nuclear transcription factors, which are tightly regulated by interaction with regulatory proteins such as Grb2 and SLAP. We reported recently that TCR stimulation induces the expression of cytokine-induced SH2 protein (CIS). The expression of CIS promotes TCR-mediated activation. We have now found specific interactions between CIS and activated protein kinase C (PKC) alpha, beta and theta in TCR-stimulated T cells. CIS was shown by in vitro kinase assay to associate with activated PKC. In CIS-expressing T cells isolated from CIS-transgenic mice, the amount of activated PKC associated with CIS was found to increase following TCR stimulation. By immunohistochemical analysis,... (More)
TCR signaling is mediated by intracellular signaling molecules and nuclear transcription factors, which are tightly regulated by interaction with regulatory proteins such as Grb2 and SLAP. We reported recently that TCR stimulation induces the expression of cytokine-induced SH2 protein (CIS). The expression of CIS promotes TCR-mediated activation. We have now found specific interactions between CIS and activated protein kinase C (PKC) alpha, beta and theta in TCR-stimulated T cells. CIS was shown by in vitro kinase assay to associate with activated PKC. In CIS-expressing T cells isolated from CIS-transgenic mice, the amount of activated PKC associated with CIS was found to increase following TCR stimulation. By immunohistochemical analysis, CIS was also found to co-localize with PKCtheta at the plasma membrane of activated T cells. In addition to the interaction and intracellular co-localization of the CIS and PKC, an increase in the activation of AP-1 and NF-kappaB was noted in CIS-expressing T cells, after stimulation by either anti-CD3/CD28 or phorbol myristate acetate + ionomycin. These results suggest that CIS regulates PKC activation, and that this may be important for the activation of both the AP-1 and NF-kappaB pathways in TCR signaling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
TCR, signal transduction, protein kinase C, NF-kappa B, AP-1, cytokine-induced SH2 protein
in
International Immunology
volume
15
issue
3
pages
403 - 409
publisher
Oxford University Press
external identifiers
  • wos:000181546600010
  • pmid:12618484
  • scopus:0037342547
ISSN
1460-2377
DOI
10.1093/intimm/dxg039
language
English
LU publication?
yes
id
3310f352-c05a-4466-b916-bf94580b887e (old id 315817)
alternative location
http://intimm.oxfordjournals.org/cgi/content/abstract/15/3/403
date added to LUP
2007-08-28 09:05:01
date last changed
2018-07-15 03:31:01
@article{3310f352-c05a-4466-b916-bf94580b887e,
  abstract     = {TCR signaling is mediated by intracellular signaling molecules and nuclear transcription factors, which are tightly regulated by interaction with regulatory proteins such as Grb2 and SLAP. We reported recently that TCR stimulation induces the expression of cytokine-induced SH2 protein (CIS). The expression of CIS promotes TCR-mediated activation. We have now found specific interactions between CIS and activated protein kinase C (PKC) alpha, beta and theta in TCR-stimulated T cells. CIS was shown by in vitro kinase assay to associate with activated PKC. In CIS-expressing T cells isolated from CIS-transgenic mice, the amount of activated PKC associated with CIS was found to increase following TCR stimulation. By immunohistochemical analysis, CIS was also found to co-localize with PKCtheta at the plasma membrane of activated T cells. In addition to the interaction and intracellular co-localization of the CIS and PKC, an increase in the activation of AP-1 and NF-kappaB was noted in CIS-expressing T cells, after stimulation by either anti-CD3/CD28 or phorbol myristate acetate + ionomycin. These results suggest that CIS regulates PKC activation, and that this may be important for the activation of both the AP-1 and NF-kappaB pathways in TCR signaling.},
  author       = {Chen, S and Anderson, Per and Li, Li and Sjögren, Hans Olov and Wang, P and Li, Su-Ling},
  issn         = {1460-2377},
  keyword      = {TCR,signal transduction,protein kinase C,NF-kappa B,AP-1,cytokine-induced SH2 protein},
  language     = {eng},
  number       = {3},
  pages        = {403--409},
  publisher    = {Oxford University Press},
  series       = {International Immunology},
  title        = {Functional association of cytokine-induced SH2 protein and protein kinase C in activated T cells},
  url          = {http://dx.doi.org/10.1093/intimm/dxg039},
  volume       = {15},
  year         = {2003},
}